Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ES276_RS09750 Genome accession   NZ_CP038252
Coordinates   1930898..1931839 (-) Length   313 a.a.
NCBI ID   WP_000249564.1    Uniprot ID   A0A0H2US26
Organism   Streptococcus pneumoniae strain TVO_1901936     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1925898..1936839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES276_RS09710 (ES276_10325) comGH 1926384..1927337 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  ES276_RS09715 (ES276_10330) - 1927398..1927992 (-) 595 Protein_1916 class I SAM-dependent methyltransferase -
  ES276_RS09720 (ES276_10335) comGG/cglG 1928129..1928542 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  ES276_RS09725 (ES276_10340) comGF/cglF 1928520..1928981 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  ES276_RS09730 (ES276_10345) comGE/cglE 1928944..1929246 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  ES276_RS09735 (ES276_10350) comGD/cglD 1929209..1929613 (-) 405 WP_000588028.1 competence type IV pilus minor pilin ComGD Machinery gene
  ES276_RS09740 (ES276_10355) comGC/cglC 1929606..1929932 (-) 327 WP_000738627.1 comG operon protein ComGC Machinery gene
  ES276_RS09745 (ES276_10360) comGB/cglB 1929934..1930950 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  ES276_RS09750 (ES276_10365) comGA/cglA/cilD 1930898..1931839 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  ES276_RS09755 (ES276_10370) - 1931915..1932280 (-) 366 WP_000286415.1 DUF1033 family protein -
  ES276_RS09760 (ES276_10375) - 1932431..1933489 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  ES276_RS09765 (ES276_10380) nagA 1933652..1934803 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  ES276_RS09770 (ES276_10385) - 1934956..1936773 (-) 1818 WP_001220855.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35555.47 Da        Isoelectric Point: 6.1330

>NTDB_id=310276 ES276_RS09750 WP_000249564.1 1930898..1931839(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain TVO_1901936]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDHKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=310276 ES276_RS09750 WP_000249564.1 1930898..1931839(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain TVO_1901936]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCATAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2US26

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae D39

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae R6

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

100

100

1
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

95.847

100

0.958
  comGA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comGA Streptococcus mutans UA159

65.916

99.361

0.655
  comGA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment