Detailed information NTG0310271

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: ES276_RS09725
• Genome accession: NZ_CP038252
• Coordinates: 1928520..1928981 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250534.1
• Uniprot ID: A0A0H2US22
• Organism: Streptococcus pneumoniae strain TVO_1901936
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1923520..1933981

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ES276_RS09695 (ES276_10310)	-	1923824..1924648 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
ES276_RS09700 (ES276_10315)	rnpA	1924623..1924994 (-)	372	WP_000739253.1	ribonuclease P protein component	-
ES276_RS10870	-	1925011..1925142 (-)	132	WP_000768904.1	hypothetical protein	-
ES276_RS09705 (ES276_10320)	-	1925143..1926333 (-)	1191	WP_000167757.1	acetate kinase	-
ES276_RS09710 (ES276_10325)	comGH	1926384..1927337 (-)	954	WP_000345135.1	class I SAM-dependent methyltransferase	Machinery gene
ES276_RS09715 (ES276_10330)	-	1927398..1927992 (-)	595	Protein_1916	class I SAM-dependent methyltransferase	-
ES276_RS09720 (ES276_10335)	comGG/cglG	1928129..1928542 (-)	414	WP_000265622.1	competence type IV pilus minor pilin ComGG	Machinery gene
ES276_RS09725 (ES276_10340)	comGF/cglF	1928520..1928981 (-)	462	WP_000250534.1	competence type IV pilus minor pilin ComGF	Machinery gene
ES276_RS09730 (ES276_10345)	comGE/cglE	1928944..1929246 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
ES276_RS09735 (ES276_10350)	comGD/cglD	1929209..1929613 (-)	405	WP_000588028.1	competence type IV pilus minor pilin ComGD	Machinery gene
ES276_RS09740 (ES276_10355)	comGC/cglC	1929606..1929932 (-)	327	WP_000738627.1	comG operon protein ComGC	Machinery gene
ES276_RS09745 (ES276_10360)	comGB/cglB	1929934..1930950 (-)	1017	WP_074196785.1	competence type IV pilus assembly protein ComGB	Machinery gene
ES276_RS09750 (ES276_10365)	comGA/cglA/cilD	1930898..1931839 (-)	942	WP_000249564.1	competence type IV pilus ATPase ComGA	Machinery gene
ES276_RS09755 (ES276_10370)	-	1931915..1932280 (-)	366	WP_000286415.1	DUF1033 family protein	-
ES276_RS09760 (ES276_10375)	-	1932431..1933489 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17972.67 Da
• Isoelectric Point: 9.6092

>NTDB_id=310271 ES276_RS09725 WP_000250534.1 1928520..1928981(-) (comGF/cglF) [Streptococcus pneumoniae strain TVO_1901936]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=310271 ES276_RS09725 WP_000250534.1 1928520..1928981(-) (comGF/cglF) [Streptococcus pneumoniae strain TVO_1901936]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGATGATTTCCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGACTCAAATCTGTACGGATTACAGAGGACAATCAACTGGTTCGCTTTCATT
TCCAGTTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (45-141)

3D structure

• AlphaFold DB: A0A0H2US22

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	100	100	1
comGF/cglF	Streptococcus pneumoniae D39	100	100	1
comGF/cglF	Streptococcus pneumoniae R6	100	100	1
comGF/cglF	Streptococcus pneumoniae TIGR4	100	100	1
comGF/cglF	Streptococcus mitis SK321	90.85	100	0.909
comGF/cglF	Streptococcus mitis NCTC 12261	89.542	100	0.895