Detailed information    

experimental Experimentally validated

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   HI_0366 Genome accession   L42023
Coordinates   390026..390565 (+) Length   179 a.a.
NCBI ID   AAC22024.1    Uniprot ID   -
Organism   Haemophilus influenzae Rd KW20     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

The pilF2 mutant significantly decreased transformation rate.


Genomic Context

Location: 385026..395565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HI_0361 - 385482..386402 (-) 921 AAC22020.1 iron (chelated) transporter, ATP-binding protein (yfeB) -
  HI_0362 - 386402..387283 (-) 882 AAC22021.1 iron (chelated) ABC transporter, periplasmic-binding protein (yfeA) -
  HI_0364 - 387648..388526 (-) 879 AAC22022.1 penicillin-binding protein 7, putative -
  HI_0365 - 388752..389924 (+) 1173 AAC22023.1 conserved hypothetical protein -
  HI_0366 pilF/pilF2 390026..390565 (+) 540 AAC22024.1 fimbrial biogenesis and twitching motility protein, putative Machinery gene
  HI_0367 - 390637..391548 (+) 912 AAC22025.1 conserved hypothetical protein -
  HI_0368 - 391558..392664 (+) 1107 AAC22026.1 gcpE protein (gcpE) -
  HI_0369 - 392674..393945 (+) 1272 AAC22027.1 histidyl-tRNA synthetase (hisS) -
  HI_0370 - 393962..394576 (+) 615 AAC22028.1 conserved hypothetical protein -
  HI_0371 - 394627..394821 (-) 195 AAC22029.1 conserved hypothetical protein -
  HI_0372 - 394821..395162 (-) 342 AAC22030.1 ferredoxin (fdx-1) -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20598.25 Da        Isoelectric Point: 8.6216

>NTDB_id=1358 HI_0366 AAC22024.1 390026..390565(+) (pilF/pilF2) [Haemophilus influenzae Rd KW20]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQTAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1358 HI_0366 AAC22024.1 390026..390565(+) (pilF/pilF2) [Haemophilus influenzae Rd KW20]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAACTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment    



References

[1] Sunita Sinha et al. (2012) Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for natural transformation in Haemophilus influenzae. Journal of Bacteriology 194(19):5245-54. [PMID: 22821979]
[2] Timothy M VanWagoner et al. (2004) Characterization of three new competence-regulated operons in Haemophilus influenzae. Journal of Bacteriology 186(19):6409-21. [PMID: 15375121]