Detailed information    

experimental Experimentally validated

Overview

Name   pilF   Type   Machinery gene
Locus tag   A4U84_RS02735 Genome accession   NZ_CP015099
Coordinates   612922..613503 (+) Length   193 a.a.
NCBI ID   WP_005710402.1    Uniprot ID   A0A143CGR5
Organism   Glaesserella parasuis strain SC1401     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.


Genomic Context

Location: 607922..618503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4U84_RS02695 (A4U84_02695) gltX 608533..609972 (-) 1440 WP_062923966.1 glutamate--tRNA ligase -
  A4U84_RS02720 (A4U84_02720) - 610811..611149 (+) 339 WP_062923967.1 toxin -
  A4U84_RS02725 (A4U84_02725) - 611153..611461 (+) 309 WP_021117805.1 helix-turn-helix domain-containing protein -
  A4U84_RS02730 (A4U84_02730) A4U84_RS02730 611662..612822 (+) 1161 WP_010786053.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN Unclear
  A4U84_RS02735 (A4U84_02735) pilF 612922..613503 (+) 582 WP_005710402.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  A4U84_RS02740 (A4U84_02740) - 613564..614499 (+) 936 WP_005710401.1 KpsF/GutQ family sugar-phosphate isomerase -
  A4U84_RS02745 (A4U84_02745) greB 614499..614987 (+) 489 WP_005710400.1 transcription elongation factor GreB -
  A4U84_RS02765 (A4U84_02765) - 615515..616813 (+) 1299 WP_005710399.1 NAD(P)/FAD-dependent oxidoreductase -
  A4U84_RS02770 (A4U84_02770) - 616900..617433 (-) 534 WP_010786060.1 TIGR00645 family protein -
  A4U84_RS02775 (A4U84_02775) - 617522..618160 (-) 639 WP_021112014.1 nitroreductase family protein -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22324.27 Da        Isoelectric Point: 8.0872

>NTDB_id=1386 A4U84_RS02735 WP_005710402.1 612922..613503(+) (pilF) [Glaesserella parasuis strain SC1401]
MTFAKFFRNLTACSVALWLVACANQTTQVDFNRSEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKLFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=1386 A4U84_RS02735 WP_005710402.1 612922..613503(+) (pilF) [Glaesserella parasuis strain SC1401]
ATGACCTTTGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCATTATGGCTTGTTGCTTGTGCGAACCAAACTAC
TCAGGTGGATTTTAACCGCTCGGAAGCCGTCAAAGCTCGCATTAATTTGGCCCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACACGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTAAATAACTACGGTACCTTTCTGTGCAAACAAAAGCTGTTCGACAAAGCTTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTACAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A143CGR5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

44.693

100

0.447

Multiple sequence alignment    



References

[1] Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]