Detailed information    

experimental Experimentally validated

Overview

Name   A4U84_RS02730   Type   Unclear
Locus tag   A4U84_RS02730 Genome accession   NZ_CP015099
Coordinates   611662..612822 (+) Length   386 a.a.
NCBI ID   WP_010786053.1    Uniprot ID   A0A837B0I3
Organism   Glaesserella parasuis strain SC1401     
Function   require for natural transformation   
Unclear

Function

The expression levels of candidate uptake components were significantly lower than in SC1401, respectively. These results revealed that the highly naturally competent strain SC1401 up-regulated most of the candidate natural competence-related regulons/components.


Genomic Context

Location: 606662..617822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4U84_RS02685 (A4U84_02685) - 606930..607845 (+) 916 Protein_540 transposase -
  A4U84_RS10995 - 607806..608066 (+) 261 Protein_541 cation-transporting ATPase PacS -
  A4U84_RS02695 (A4U84_02695) gltX 608533..609972 (-) 1440 WP_062923966.1 glutamate--tRNA ligase -
  A4U84_RS02720 (A4U84_02720) - 610811..611149 (+) 339 WP_062923967.1 toxin -
  A4U84_RS02725 (A4U84_02725) - 611153..611461 (+) 309 WP_021117805.1 helix-turn-helix domain-containing protein -
  A4U84_RS02730 (A4U84_02730) A4U84_RS02730 611662..612822 (+) 1161 WP_010786053.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN Unclear
  A4U84_RS02735 (A4U84_02735) pilF 612922..613503 (+) 582 WP_005710402.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  A4U84_RS02740 (A4U84_02740) - 613564..614499 (+) 936 WP_005710401.1 KpsF/GutQ family sugar-phosphate isomerase -
  A4U84_RS02745 (A4U84_02745) greB 614499..614987 (+) 489 WP_005710400.1 transcription elongation factor GreB -
  A4U84_RS02765 (A4U84_02765) - 615515..616813 (+) 1299 WP_005710399.1 NAD(P)/FAD-dependent oxidoreductase -
  A4U84_RS02770 (A4U84_02770) - 616900..617433 (-) 534 WP_010786060.1 TIGR00645 family protein -

Sequence

Protein


Download         Length: 386 a.a.        Molecular weight: 43492.86 Da        Isoelectric Point: 6.7466

>NTDB_id=1392 A4U84_RS02730 WP_010786053.1 611662..612822(+) (A4U84_RS02730) [Glaesserella parasuis strain SC1401]
MSEQIQATDASCNSTKTEKINLLNLNRQQMRELFAEMGEKPFRADQLMKWIYHFGEDNFDNMTNINKVLREKLKRIAEIK
APEVAVEQRSADGTIKWAMWVGDQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVSEIIGQVWRASKI
IGNFGVTGIRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDKMREQIDVALAISLHAPN
DELRNELIPLNKKYNIKMLIDSVNKYLEVSNANHGKVTIEYVMLSHVNDDVEHAHQLADVLKNTPSKINLIPWNPFPEAP
YAKSSNTRIDRFQKTLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTFEKRKFGEGIAVQNQ

Nucleotide


Download         Length: 1161 bp        

>NTDB_id=1392 A4U84_RS02730 WP_010786053.1 611662..612822(+) (A4U84_RS02730) [Glaesserella parasuis strain SC1401]
ATGTCTGAACAAATTCAAGCAACCGATGCAAGTTGTAATTCAACCAAAACTGAAAAAATTAACTTATTAAACTTAAATCG
TCAGCAGATGCGTGAACTATTTGCGGAAATGGGCGAGAAGCCATTTCGTGCTGATCAGCTGATGAAATGGATTTATCATT
TTGGTGAAGATAATTTTGATAATATGACCAACATCAATAAGGTGTTGCGTGAAAAATTAAAACGGATCGCTGAGATTAAA
GCACCTGAAGTTGCCGTAGAACAACGTTCTGCCGACGGCACAATCAAATGGGCGATGTGGGTTGGCGATCAGCAAATTGA
AACGGTATATATCCCAGAAGATGATCGTGCTACCTTGTGTGTTTCTTCGCAGGTAGGTTGTGCCTTGGCTTGTACTTTCT
GTTCAACGGCACAGCAAGGCTTTAACCGTAATTTAAGCGTATCTGAAATTATCGGGCAGGTGTGGCGTGCATCAAAAATT
ATCGGTAACTTTGGTGTAACGGGCATTCGTCCGATTACCAATGTGGTGATGATGGGAATGGGTGAACCATTGTTGAATAT
GAATAACGTTATTCCAGCAATGGAAATTATGTTAGACGATTTTGCTTATGGTCTATCTAAACGCCGTGTGACTCTTTCAA
CATCAGGGGTTGTACCAGCGTTAGATAAAATGCGTGAGCAGATTGATGTTGCATTAGCGATTTCATTGCACGCACCAAAC
GATGAACTGCGTAATGAATTAATTCCACTGAATAAAAAATACAACATTAAAATGTTAATTGATTCAGTGAATAAATACTT
AGAAGTATCAAACGCTAATCACGGTAAAGTGACGATTGAATATGTGATGCTAAGCCACGTCAATGATGATGTGGAACACG
CTCATCAGTTGGCAGATGTATTGAAAAATACCCCAAGTAAAATCAACTTGATCCCGTGGAACCCGTTCCCAGAAGCACCC
TATGCGAAGAGTTCAAATACGCGTATTGATCGCTTCCAAAAAACCTTGATGGAATATGGCTTTACGGTGACAGTGCGTAA
AACTCGTGGTGATGATATTGATGCTGCCTGTGGGCAACTTGCGGGTGATGTGATTGACCGCACTAAACGCACCTTTGAAA
AACGTAAATTCGGTGAAGGGATTGCGGTTCAAAATCAGTAG

Domains

Predicted by InterproScan.

Radical SAM superfamily (Radical_SAM)

(122-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A837B0I3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Ke Dai et al. (2018) Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401. Frontiers in Cellular And Infection Microbiology 8:32. [PMID: 29473023]