Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CH556_RS03590 Genome accession   NZ_CP031692
Coordinates   703528..704922 (+) Length   464 a.a.
NCBI ID   WP_116945235.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P595-8370     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 698528..709922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH556_RS03565 (CH556_03565) rsmE 698542..699279 (-) 738 WP_038439236.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CH556_RS03570 (CH556_03570) lnt 699329..700858 (-) 1530 WP_116945309.1 apolipoprotein N-acyltransferase -
  CH556_RS03575 (CH556_03575) corC 700881..701780 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  CH556_RS03580 (CH556_03580) ampD 702414..702968 (-) 555 WP_116945237.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CH556_RS03585 (CH556_03585) pilA 703082..703531 (+) 450 WP_116945236.1 prepilin peptidase-dependent pilin Machinery gene
  CH556_RS03590 (CH556_03590) pilB 703528..704922 (+) 1395 WP_116945235.1 GspE/PulE family protein Machinery gene
  CH556_RS03595 (CH556_03595) pilC 704919..706139 (+) 1221 WP_116945234.1 type II secretion system F family protein Machinery gene
  CH556_RS03600 (CH556_03600) pilD 706136..706828 (+) 693 WP_116945233.1 A24 family peptidase Machinery gene
  CH556_RS03605 (CH556_03605) rho 706882..708144 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  CH556_RS03610 (CH556_03610) metJ 708392..708709 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  CH556_RS03615 (CH556_03615) cueR 708723..709109 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  CH556_RS03620 (CH556_03620) - 709186..709392 (+) 207 WP_005648955.1 heavy-metal-associated domain-containing protein -
  CH556_RS03625 (CH556_03625) - 709478..709684 (+) 207 WP_005686717.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52903.37 Da        Isoelectric Point: 5.6599

>NTDB_id=310275 CH556_RS03590 WP_116945235.1 703528..704922(+) (pilB) [Haemophilus influenzae strain P595-8370]
MTSYALLHTQRVTAKNGEVFTISPDLWERNRQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPIEPIL
LESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLANSCDCHQGYKGRIGVYQFLHWQQNGYQTDFENLRDSGLEKVNQGMTDNKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=310275 CH556_RS03590 WP_116945235.1 703528..704922(+) (pilB) [Haemophilus influenzae strain P595-8370]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCGGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTCTGGC
TAGGGGTTGATTCTCTCTCTAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACGGGAAAACCTATCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGTGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCTTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGTGCGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCCACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATCATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGCCAAGATCCCGACATCATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACCGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCTATATCTCGCTTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAGCAAATTCTTGTGATTGCCATCAAGGTTATAAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAAAATGGCTATCAAACGGATTTTGAGAATTTACGAGATAGCGGTCTAGAAAAAGTTAATCAAGGAATGACAGATAATA
AAGAAATTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-95)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.767

100

0.968
  pilB Haemophilus influenzae Rd KW20

96.544

99.784

0.963
  pilB Glaesserella parasuis strain SC1401

57.456

98.276

0.565
  pilB Vibrio cholerae strain A1552

39.089

100

0.444
  pilB Vibrio campbellii strain DS40M4

40.286

100

0.425
  pilB Acinetobacter baylyi ADP1

38.911

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

39.752

100

0.414
  pilB Legionella pneumophila strain ERS1305867

38.945

100

0.414
  pilB Acinetobacter baumannii D1279779

39.381

100

0.412
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.821

100

0.381
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilF Thermus thermophilus HB27

35.714

100

0.366

Multiple sequence alignment