Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NPUN_RS00615 Genome accession   NC_010628
Coordinates   164328..165455 (-) Length   375 a.a.
NCBI ID   WP_012406947.1    Uniprot ID   -
Organism   Nostoc punctiforme PCC 73102     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 159328..170455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPUN_RS00585 (Npun_R0113) - 159656..159865 (-) 210 WP_012406943.1 DUF2997 domain-containing protein -
  NPUN_RS00600 (Npun_F0114) bioU 161137..162141 (+) 1005 WP_012406944.1 (S)-8-amino-7-oxononanoate synthase BioU -
  NPUN_RS00605 (Npun_R0115) - 162318..162878 (-) 561 WP_012406945.1 hypothetical protein -
  NPUN_RS00610 (Npun_R0116) - 163000..164223 (-) 1224 WP_012406946.1 type II secretion system F family protein -
  NPUN_RS00615 (Npun_R0117) pilT 164328..165455 (-) 1128 WP_012406947.1 type IV pilus twitching motility protein PilT Machinery gene
  NPUN_RS00620 (Npun_R0118) pilB/pilB1 165566..167572 (-) 2007 WP_012406948.1 GspE/PulE family protein Machinery gene
  NPUN_RS00625 (Npun_F0119) - 167814..168059 (+) 246 WP_012406949.1 hypothetical protein -
  NPUN_RS42890 (Npun_F0120) - 168387..168539 (+) 153 WP_234711023.1 hypothetical protein -
  NPUN_RS00635 (Npun_F0121) grpE 168889..169638 (+) 750 WP_041565100.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41040.30 Da        Isoelectric Point: 7.4775

>NTDB_id=30779 NPUN_RS00615 WP_012406947.1 164328..165455(-) (pilT) [Nostoc punctiforme PCC 73102]
MEMMIEDLMEQLIEMGGSDMHLSAGLPPYFRISGKLTPIGEHVLTADQCQRLIFSMLNNTQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGSYAACLRALSSKIPNFEKLGLPDIVREMTDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVSKKNPKPGEYGRVMAQEILVVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADFYKAGTISFEAAMSKTSKPDEIQRLIGNSTPQAAAGAKPGAAAKAH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=30779 NPUN_RS00615 WP_012406947.1 164328..165455(-) (pilT) [Nostoc punctiforme PCC 73102]
ATGGAAATGATGATTGAAGACTTGATGGAGCAGTTGATTGAAATGGGTGGCTCGGATATGCATTTATCCGCAGGTTTACC
TCCCTACTTCCGTATCAGTGGCAAACTCACTCCCATCGGTGAGCATGTATTGACAGCCGATCAATGTCAAAGGCTGATTT
TTAGTATGCTCAACAACACCCAACGTAAAACCTTAGAGCAGAACTGGGAATTGGATTGTTCTTATGGTGTTAAGGGTTTG
GCACGTTTTCGGGTCAATGTCTATAAAGAACGTGGTTCTTATGCTGCTTGCTTACGGGCATTAAGTTCTAAGATTCCTAA
CTTTGAAAAGTTAGGTCTGCCAGATATTGTACGGGAAATGACAGATAAGCCCAGAGGATTAATTCTGGTGACAGGCCCTA
CAGGTTCCGGTAAGACAACTACCCTAGCGGCAATGATCGACTTGATTAACCGCACTAAGGCAGAGCATATTTTAACGGTG
GAAGACCCAATTGAATTTGTCTACGAACCCATTAAAAGCTTGGTTCACCAACGACAACTGGGTGAAGATACTAAGAGCTT
TGCTAACGCTTTGAAAGCAGCTTTGCGGGAAGATCCAGATATTGTTCTAGTGGGAGAAATGCGCGATTTGGAAACGATTT
CTTTGGCGATTTCCGCAGCAGAAACAGGACACTTAGTATTTGGCACGCTACACACCAGTTCCGCCGCCCAAACAGTTGAC
CGGATTATCGACGTTTTCCCCCATGAAAGACAAACCCAAGTCCGGGTGCAGTTATCTAACTCGTTAGTAGCGGTATTTAG
TCAAACTTTGGTGTCCAAGAAAAACCCTAAACCTGGTGAATATGGTCGGGTGATGGCTCAAGAAATTCTGGTTGTCACTC
CTGCTATTTCCAACTTGATTCGAGAAGGCAAAACATCTCAAATTTACTCAGCTATTCAAACTGGCGGCAAATTGGGGATG
CAAACTCTGGAGAAGGTTTTAGCTGATTTTTACAAAGCAGGAACGATTTCCTTTGAAGCAGCGATGTCTAAGACTTCTAA
GCCTGATGAAATCCAACGTCTGATTGGTAATTCTACACCACAAGCCGCCGCAGGTGCGAAGCCTGGTGCGGCTGCCAAAG
CACATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.179

92.533

0.501
  pilT Pseudomonas stutzeri DSM 10701

53.582

93.067

0.499
  pilT Legionella pneumophila strain ERS1305867

52.738

92.533

0.488
  pilT Legionella pneumophila strain Lp02

52.738

92.533

0.488
  pilT Acinetobacter baylyi ADP1

52.45

92.533

0.485
  pilT Acinetobacter baumannii D1279779

51.585

92.533

0.477
  pilT Acinetobacter baumannii strain A118

51.585

92.533

0.477
  pilT Acinetobacter nosocomialis M2

51.585

92.533

0.477
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.169

94.667

0.456
  pilT Neisseria gonorrhoeae MS11

50.442

90.4

0.456
  pilT Neisseria meningitidis 8013

50.147

90.4

0.453
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.2

0.451
  pilT Vibrio cholerae strain A1552

49.415

91.2

0.451

Multiple sequence alignment