Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NPUN_RS00620 Genome accession   NC_010628
Coordinates   165566..167572 (-) Length   668 a.a.
NCBI ID   WP_012406948.1    Uniprot ID   B2J3I9
Organism   Nostoc punctiforme PCC 73102     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 160566..172572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPUN_RS00600 (Npun_F0114) bioU 161137..162141 (+) 1005 WP_012406944.1 (S)-8-amino-7-oxononanoate synthase BioU -
  NPUN_RS00605 (Npun_R0115) - 162318..162878 (-) 561 WP_012406945.1 hypothetical protein -
  NPUN_RS00610 (Npun_R0116) - 163000..164223 (-) 1224 WP_012406946.1 type II secretion system F family protein -
  NPUN_RS00615 (Npun_R0117) pilT 164328..165455 (-) 1128 WP_012406947.1 type IV pilus twitching motility protein PilT Machinery gene
  NPUN_RS00620 (Npun_R0118) pilB/pilB1 165566..167572 (-) 2007 WP_012406948.1 GspE/PulE family protein Machinery gene
  NPUN_RS00625 (Npun_F0119) - 167814..168059 (+) 246 WP_012406949.1 hypothetical protein -
  NPUN_RS42890 (Npun_F0120) - 168387..168539 (+) 153 WP_234711023.1 hypothetical protein -
  NPUN_RS00635 (Npun_F0121) grpE 168889..169638 (+) 750 WP_041565100.1 nucleotide exchange factor GrpE -
  NPUN_RS00640 (Npun_F0122) dnaK 169865..171823 (+) 1959 WP_012406951.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 668 a.a.        Molecular weight: 74534.29 Da        Isoelectric Point: 5.1727

>NTDB_id=30780 NPUN_RS00620 WP_012406948.1 165566..167572(-) (pilB/pilB1) [Nostoc punctiforme PCC 73102]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYVNTEQMRQALIESRKSGRPLTEVLESITGQQLSPELLRQYKKQQLFELK
ILYGVEFLDPEVNSIGNTMMGNLIETLIPVDICRRHRLVPLSKHEDQTPPSVLVAMVAPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDEMAVRQKHLEQEKFTDINQDLENLGNLDLADAPEEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEGVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPDTLHIVQDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRVPYTPTTEELGRYGLSASSDVGVTFYKANTLTLDAIAEAKGKNQLCSKCNG
VGYKGRCGVYEVMRVTENLQTLINEDAPTERIKEVAIEEGMKTLLAYSLDLVREGSTTLEEVERVTFTDTGLEAELKAKR
KTGLTCRSCDATLKQEWLDCPYCMTSRF

Nucleotide


Download         Length: 2007 bp        

>NTDB_id=30780 NPUN_RS00620 WP_012406948.1 165566..167572(-) (pilB/pilB1) [Nostoc punctiforme PCC 73102]
ATGACTTACTCGTCACCACAACGGCGCAGTACCGCTCTAACTACCAGAACAGAGTTTTCGCCCTTTGGCAACAAGCTAGT
GCAATCTGGCTATGTCAATACCGAACAGATGAGGCAAGCACTAATTGAAAGCCGCAAATCTGGCCGACCCTTAACGGAAG
TACTAGAGTCAATCACTGGGCAACAACTATCACCTGAGTTGCTCAGGCAATACAAAAAACAGCAGCTATTTGAACTTAAA
ATACTATACGGTGTTGAATTTCTAGATCCGGAAGTCAACTCCATTGGCAACACGATGATGGGGAACCTGATTGAAACCCT
CATCCCAGTGGATATCTGCCGTCGCCATCGTTTAGTACCACTATCGAAACACGAAGACCAAACCCCGCCCTCAGTTTTAG
TGGCGATGGTCGCTCCAGATAATCTAGAAGCTTCCGATGACCTGAACCGCATCTTGCGTCCCCAAGGGCTCGCATTACAG
CGCATGGTGATCACACAGGAAGACTACCAGCAGCTAATCAACCAATATCTGGATGAAATGGCTGTTCGGCAAAAACACCT
GGAACAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAACCTCGGAAATCTCGATCTTGCAGATGCTCCTGAAGAAA
TGGAGGCTGATCTAGGGGCAGCAATGAAGGGTGCAGAGGATGCCCCAGTCATTAACCTAGTCAACAGAATCCTGGCTAAA
GCCTTGCATGAGGGTGTTTCTGACATTCATATCGAACCGCAAGAAGAAAACTTACGCATTCGCTTTCGGAAGGATGGGGT
ACTGCGTGAAGCTTTTGATCCCCTACCGAAAAAAATCATCCCAGCGGTGACAGCCCGATTTAAAATCATCTCCAATCTAG
ACATTGCTGAACGCCGTTTACCCCAAGATGGACGCATCCGTCGGCTGTTTGAAGGACGCAAAGTTGACTTCCGTGTGAAT
ACCTTACCCAGTCGCTACGGGGAAAAGGTGGTGCTGCGGATTTTGGATAACTCCTCCACCCAACTGGGATTAGATAAGTT
AATTACCGATCCAGATACTTTACATATTGTCCAGGATATGGTTAGCCGCCCCTTTGGTCTAATTTTAGTAACTGGGCCAA
CTGGTTCTGGGAAAACAACCTCGCTGTATTCTGCACTATCAGAAAAGAACGATCCCGGAATTAATATCAGTACTGTGGAA
GACCCAATTGAGTACAGTCTTCCAGGGATTACTCAAGTACAGGTGATTCGGGAAAAAGGGCTGGATTTTGCTACCGCTCT
ACGGGCTTTCTTGAGGCAAGATCCAGATGTGCTGCTGGTAGGTGAAACGCGGGATAAGGAAACGGCAAAAACAGCGATTG
AGGCTGCCTTGACCGGCCACTTAGTATTAACTACCTTACATACCAATGATGCCCCAGGAGCGATCGCTCGTTTGGGAGAA
ATGGGGATTGAGCCTTTCATGGTTTCTAGTTCCCTAATTGGCGTTTTAGCTCAACGTTTGGTACGGCGTGTATGTTCTGA
ATGTCGTGTTCCTTACACTCCCACAACCGAGGAACTAGGTCGTTATGGTCTATCAGCTTCCTCAGATGTCGGAGTCACGT
TCTATAAGGCTAACACCTTGACATTAGATGCGATCGCCGAAGCCAAAGGCAAAAATCAGCTTTGCTCAAAATGTAATGGT
GTCGGCTACAAAGGACGTTGTGGCGTTTATGAAGTCATGCGAGTCACCGAAAACCTGCAAACTCTCATCAACGAAGATGC
ACCCACAGAACGCATCAAGGAAGTGGCAATAGAAGAGGGTATGAAAACCTTGCTGGCTTATAGTTTGGACTTAGTGCGTG
AAGGTTCTACCACTTTAGAAGAAGTAGAACGAGTGACGTTTACTGATACTGGTTTGGAAGCCGAGTTAAAAGCCAAACGC
AAGACTGGTCTTACCTGCCGGAGTTGCGATGCCACATTGAAACAAGAATGGCTGGATTGTCCATACTGTATGACATCTCG
GTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2J3I9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.22

100

0.666
  pilF Thermus thermophilus HB27

39.478

91.766

0.362

Multiple sequence alignment