Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DVJ83_RS01605 Genome accession   NZ_CP031158
Coordinates   300403..301659 (+) Length   418 a.a.
NCBI ID   WP_164993995.1    Uniprot ID   -
Organism   Deinococcus wulumuqiensis strain NEB 479     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 295403..306659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DVJ83_RS01590 (DVJ83_01590) pgeF 296205..296954 (+) 750 WP_114671145.1 peptidoglycan editing factor PgeF -
  DVJ83_RS01595 (DVJ83_01595) - 296951..297460 (+) 510 WP_114671146.1 YqeG family HAD IIIA-type phosphatase -
  DVJ83_RS01600 (DVJ83_01600) pilB 297695..300358 (+) 2664 WP_162865304.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  DVJ83_RS01605 (DVJ83_01605) pilT 300403..301659 (+) 1257 WP_164993995.1 type IV pilus twitching motility protein PilT Machinery gene
  DVJ83_RS01610 (DVJ83_01610) - 301771..302283 (-) 513 WP_114671148.1 hypothetical protein -
  DVJ83_RS01615 (DVJ83_01615) - 302540..305182 (+) 2643 WP_114671149.1 S8 family serine peptidase -
  DVJ83_RS01620 (DVJ83_01620) - 305521..306177 (-) 657 WP_162865305.1 ankyrin repeat domain-containing protein -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 44731.01 Da        Isoelectric Point: 6.7738

>NTDB_id=303800 DVJ83_RS01605 WP_164993995.1 300403..301659(+) (pilT) [Deinococcus wulumuqiensis strain NEB 479]
MTSGIDITDILRVAADQGASDVILAAGLPPQFKLSGTYDSQGFEALSPTDTRKLMYSMMNERQQRTFEEKRELDFSFALG
DKARFRVNTFMQRGNVGGVMRLIPTTVRTIAEMGLPQTVIDIASAPRGLVLVTGPTGSGKSTTLASMIDHINTTKKLHIL
TIEDPIEFMHPNKASIINQREVGADTLSFDDALRAALRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPES
IDRIVDVFPEEQQEQIRVQLANNLVAVMTQQLLPRADGQGRVLAYEILLANPAVRALIREGKTFQITSVMQTGAREGMVT
MDAYLAGLFRKHLITYDKGLERAIDPKEFARLANDPSAGLGGAAMTPAAPPVPAYSSAASSGAGRGGDFGRGAAAPAAPA
TPASGRNDPGRPGGFGRR

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=303800 DVJ83_RS01605 WP_164993995.1 300403..301659(+) (pilT) [Deinococcus wulumuqiensis strain NEB 479]
ATGACTTCCGGTATCGACATCACCGACATCCTGCGCGTCGCCGCCGACCAGGGCGCGTCCGACGTCATTCTCGCCGCCGG
GCTGCCCCCGCAGTTCAAGCTCTCGGGCACCTACGACTCGCAGGGCTTCGAAGCCCTGTCGCCCACCGACACCCGCAAGC
TGATGTACTCGATGATGAACGAGCGCCAGCAGCGCACCTTCGAGGAAAAGCGCGAACTCGACTTTTCCTTCGCGCTGGGC
GACAAGGCCCGTTTCCGCGTGAACACCTTCATGCAGCGCGGCAACGTGGGCGGCGTCATGCGACTGATTCCCACCACCGT
CCGCACCATCGCCGAAATGGGGCTGCCGCAGACCGTCATCGACATCGCGAGCGCCCCGCGTGGGCTGGTGCTGGTGACGG
GGCCGACGGGGTCGGGCAAATCGACCACCCTGGCGTCGATGATCGACCACATCAACACGACCAAGAAACTGCACATCCTG
ACCATCGAAGACCCCATCGAGTTCATGCACCCCAACAAGGCCAGCATCATCAACCAGCGCGAGGTCGGGGCCGACACCCT
CAGTTTCGACGACGCGCTGCGGGCCGCGCTGCGTCAGGCCCCCGACGTGATTCTGGTGGGCGAAATGCGTGACTACGAAA
CCATCAAGGCCGCCGTGACCGCCGCCGAAACCGGGCACCTCGTGATGGGCACGCTGCACACCAACTCGGCGCCCGAATCC
ATCGACCGTATCGTGGACGTGTTCCCCGAAGAGCAGCAGGAACAGATTCGCGTGCAGCTCGCCAACAACCTCGTCGCGGT
GATGACCCAGCAGCTTCTGCCCCGCGCCGACGGTCAGGGGCGCGTGCTGGCCTACGAGATTCTGCTGGCGAACCCGGCAG
TCCGCGCCCTGATTCGTGAGGGCAAGACCTTCCAGATCACCAGCGTGATGCAGACCGGCGCCCGCGAGGGCATGGTCACC
ATGGACGCCTACCTCGCCGGACTGTTCCGCAAGCACCTGATCACCTACGACAAGGGCCTGGAACGTGCCATCGACCCCAA
GGAATTCGCCCGCCTCGCCAACGACCCCTCGGCGGGTCTGGGGGGCGCGGCCATGACGCCCGCCGCGCCGCCGGTTCCGG
CCTACAGCAGCGCGGCCAGCAGCGGTGCCGGGCGCGGCGGCGACTTCGGACGGGGTGCGGCGGCCCCGGCAGCTCCGGCC
ACGCCCGCGTCAGGCCGCAACGACCCCGGACGCCCTGGGGGGTTTGGCCGCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

94.019

100

0.94
  pilT Pseudomonas stutzeri DSM 10701

52.754

82.536

0.435
  pilT Acinetobacter baylyi ADP1

52.174

82.536

0.431
  pilT Pseudomonas aeruginosa PAK

52.174

82.536

0.431
  pilT Neisseria meningitidis 8013

52.478

82.057

0.431
  pilT Acinetobacter nosocomialis M2

51.884

82.536

0.428
  pilT Acinetobacter baumannii strain A118

51.884

82.536

0.428
  pilT Acinetobacter baumannii D1279779

51.884

82.536

0.428
  pilT Neisseria gonorrhoeae MS11

52.212

81.1

0.423
  pilT Legionella pneumophila strain Lp02

48.986

82.536

0.404
  pilT Legionella pneumophila strain ERS1305867

48.986

82.536

0.404
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.201

76.077

0.397
  pilT Vibrio cholerae strain A1552

52.201

76.077

0.397

Multiple sequence alignment