Detailed information NTG0303799

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DVJ83_RS01600
• Genome accession: NZ_CP031158
• Coordinates: 297695..300358 (+)
• Length: 887 a.a.
• NCBI ID: WP_162865304.1
• Uniprot ID: -
• Organism: Deinococcus wulumuqiensis strain NEB 479
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 292695..305358

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DVJ83_RS01565 (DVJ83_01565)	-	292757..293275 (+)	519	WP_114671141.1	winged helix-turn-helix domain-containing protein	-
DVJ83_RS01570 (DVJ83_01570)	-	293449..293958 (+)	510	WP_114671142.1	IS630 family transposase	-
DVJ83_RS01575 (DVJ83_01575)	-	294196..294546 (-)	351	WP_017870157.1	winged helix-turn-helix transcriptional regulator	-
DVJ83_RS01580 (DVJ83_01580)	-	294635..295255 (+)	621	WP_114671143.1	nitroreductase family protein	-
DVJ83_RS01585 (DVJ83_01585)	-	295330..296124 (-)	795	WP_114671144.1	enoyl-ACP reductase FabI	-
DVJ83_RS01590 (DVJ83_01590)	pgeF	296205..296954 (+)	750	WP_114671145.1	peptidoglycan editing factor PgeF	-
DVJ83_RS01595 (DVJ83_01595)	-	296951..297460 (+)	510	WP_114671146.1	YqeG family HAD IIIA-type phosphatase	-
DVJ83_RS01600 (DVJ83_01600)	pilB	297695..300358 (+)	2664	WP_162865304.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
DVJ83_RS01605 (DVJ83_01605)	pilT	300403..301659 (+)	1257	WP_164993995.1	type IV pilus twitching motility protein PilT	Machinery gene
DVJ83_RS01610 (DVJ83_01610)	-	301771..302283 (-)	513	WP_114671148.1	hypothetical protein	-
DVJ83_RS01615 (DVJ83_01615)	-	302540..305182 (+)	2643	WP_114671149.1	S8 family serine peptidase	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 97069.97 Da
• Isoelectric Point: 4.8612

>NTDB_id=303799 DVJ83_RS01600 WP_162865304.1 297695..300358(+) (pilB) [Deinococcus wulumuqiensis strain NEB 479]
MALSIGDRRLGAILLDQGYLGDNDLQRALERHAEVGGRLADVLIDSGMVGEKRIARAIEEALGIPLVNLLAVQPDPAALR
SIRPQTALNLQAFPFALEGDRLRVALVDPLSSFSIETLEDDSSLDIEPYQALREEVLWALATHYPELGLDIVVPSGVSET
GRAGGKLGDRLIMHGYITDAQLQVALDAQQQTGEALGTTLITQRAITEDQLYEVLAEQEGTTFLPNPSGFHPGEEVLGSM
LRADALRLLAVPVDETEQGVTVLTSDPRKRPDIGALIGRPVQLMLTRPRDIERLIEQFYPQRGRLGEQLVQEGALSRDQL
REALQVQAREGKVKPLGEIITELGFASPDEVDSALQKQNVGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPIQNPP
DPKVALMIPESTARRYVVVPVRLQGNALVVAMKDPRNVFALDDLKLITGKDILPAVMAEKDIIRLIERYFGEKGFEKLNK
ELAERNKTQQSQEVDLAVADESAIVQVVDSIIREAALQDASDIHIETTDDGVKVRYRIDGALRDQNSFPKGAAQQIMARL
KIMGHLDIAERRIPQDGRIRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPELEQLGFSEYNYARYTEIIERPNGI
FLVTGPTGSGKSFTCFSTLKRIAKPEKNTTTIEDPIEYEVPGIVQSQVNNTTGMTFARALRAFLRQDPDIIFVGEIRDTE
TAKIAVEAALTGHMVLATLHTNDAPGAVTRLEEMGVENFNISASVMGVLAQRLVRRVCSECKQPTNADPEVLRRLGIGER
EIRGANLVRGAGCPRCGGTGYKGRMGIHELMVIDDGLRRAIGAGRPAAEIRDVALSESGLRSLRQDGIEKALQGLTTLEE
VLAATAN

Nucleotide

• Length: 2664 bp

>NTDB_id=303799 DVJ83_RS01600 WP_162865304.1 297695..300358(+) (pilB) [Deinococcus wulumuqiensis strain NEB 479]
TTGGCTCTTTCGATCGGTGACCGCCGTCTGGGCGCGATTTTGCTGGACCAGGGGTATCTGGGCGACAACGACTTGCAGCG
GGCGCTGGAGCGGCACGCCGAGGTGGGGGGGCGGCTGGCCGACGTCCTTATCGACTCGGGCATGGTGGGCGAAAAACGCA
TCGCCCGCGCCATCGAGGAAGCGCTGGGCATCCCGCTGGTCAACCTGCTGGCGGTGCAGCCCGACCCGGCGGCGCTGCGC
TCGATTCGGCCCCAGACGGCGCTCAACCTGCAGGCGTTTCCCTTCGCACTGGAGGGCGACCGGCTGCGGGTGGCGCTGGT
GGACCCGCTGTCGAGCTTTTCTATCGAGACCCTGGAGGACGACAGCAGCCTCGATATCGAACCGTATCAGGCGCTGCGCG
AGGAAGTGCTGTGGGCGCTCGCGACGCACTATCCCGAACTGGGCCTCGACATCGTGGTGCCCAGCGGCGTGTCGGAAACG
GGGCGCGCCGGGGGCAAGCTCGGTGACCGGCTGATCATGCACGGCTACATCACCGACGCGCAGCTTCAGGTGGCGCTCGA
CGCGCAGCAGCAGACCGGCGAGGCGCTCGGAACCACCCTGATCACGCAGCGGGCGATTACCGAAGACCAGCTCTACGAAG
TGCTGGCCGAGCAGGAAGGCACCACCTTCCTTCCCAACCCCAGCGGCTTTCACCCCGGCGAGGAAGTGCTCGGCAGCATG
CTCCGTGCCGACGCCCTGCGCCTGCTGGCCGTGCCGGTGGACGAAACCGAGCAGGGCGTGACGGTGCTCACCAGCGACCC
GCGCAAGCGCCCCGACATCGGCGCGCTGATCGGGCGCCCGGTGCAGCTGATGCTCACGCGCCCGCGCGACATCGAGCGCC
TGATCGAGCAGTTCTACCCCCAGCGCGGGCGCCTGGGCGAGCAGCTCGTGCAGGAAGGGGCGCTGTCACGCGATCAACTT
CGTGAGGCGCTTCAGGTCCAGGCCCGCGAGGGCAAGGTCAAGCCGCTGGGCGAGATCATCACCGAACTGGGCTTTGCCAG
CCCCGACGAGGTGGATTCGGCGCTGCAAAAGCAGAACGTGGGCGGGGGCCGCCTCGAAGACACCCTGGTGCAGTCGGGCA
AGCTCAGCCCCGAAATGCTCGCCCGCTCGCTGGCCGCGCAGCTCGGCTACGAGTTTCTCGACCCCATCCAGAACCCGCCG
GACCCCAAGGTCGCGCTGATGATTCCCGAGTCCACCGCCCGGCGGTACGTGGTGGTTCCGGTGCGTCTCCAGGGCAACGC
GCTGGTCGTCGCCATGAAGGACCCGCGCAACGTCTTCGCGCTCGACGACCTCAAGCTGATCACCGGCAAGGACATCCTGC
CCGCCGTGATGGCGGAAAAGGACATCATCCGCCTGATCGAGCGCTACTTCGGGGAAAAGGGCTTCGAGAAGCTCAACAAG
GAACTCGCCGAGCGCAACAAGACGCAGCAGTCGCAGGAAGTCGACCTCGCGGTGGCCGACGAGAGCGCCATCGTGCAGGT
CGTAGACTCGATCATCCGCGAGGCCGCGCTGCAGGACGCGTCGGACATCCACATCGAAACCACCGACGACGGCGTCAAGG
TGCGCTACCGCATCGACGGAGCGCTGCGCGACCAGAACTCCTTTCCCAAGGGCGCGGCGCAGCAGATTATGGCCCGCCTC
AAAATCATGGGGCACCTCGACATCGCCGAGCGGCGCATTCCCCAGGACGGGCGCATCCGCTTCAAAAAGGGCAGCATCGA
CCTCGACCTGCGTCTCTCGACCCTGCCCACCGTGTACGGCGAAAAGGCCGTGATGCGTCTGCTGCAAAAGGCGAGCAACA
TCCCCGAACTCGAGCAGCTCGGCTTTTCCGAGTACAACTACGCCCGCTACACCGAAATTATCGAGCGGCCCAACGGGATT
TTTCTGGTCACCGGGCCGACGGGGTCGGGCAAGTCGTTCACCTGCTTTTCGACCCTCAAGCGCATCGCCAAGCCCGAGAA
AAACACCACGACCATCGAGGACCCCATCGAGTACGAGGTGCCGGGCATCGTGCAGTCGCAGGTGAACAACACCACCGGCA
TGACGTTCGCCCGCGCGCTGCGCGCCTTTCTCCGTCAGGACCCCGACATCATCTTCGTGGGCGAGATCCGGGATACTGAA
ACCGCCAAGATTGCCGTGGAAGCCGCGCTCACCGGCCACATGGTGCTGGCGACGCTGCACACCAACGACGCGCCGGGCGC
CGTGACCCGACTGGAAGAAATGGGCGTCGAGAACTTCAACATCTCGGCGTCGGTCATGGGCGTGCTCGCGCAGCGGCTGG
TGCGCCGGGTGTGCAGCGAGTGCAAGCAGCCCACCAACGCCGACCCCGAAGTGCTGCGGCGCCTGGGCATCGGCGAGCGC
GAGATTCGCGGCGCCAACCTGGTGCGCGGGGCCGGGTGTCCGCGTTGCGGCGGCACCGGCTACAAGGGCCGCATGGGGAT
TCACGAGCTGATGGTGATTGACGACGGGCTGCGCCGCGCCATCGGGGCCGGGCGCCCCGCCGCCGAGATTCGTGACGTGG
CGCTGAGTGAAAGCGGCCTGCGCAGCCTGCGTCAGGACGGCATCGAGAAAGCCTTGCAGGGCCTGACCACCCTCGAAGAA
GTGCTGGCCGCCACCGCCAACTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-472)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (200-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

• Type II/IV secretion system protein (T2SSE) (511-776)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	95.378	100	0.954
pilF	Thermus thermophilus HB27	56.453	100	0.567