Detailed information NTG0294757

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: CSC33_RS18645
• Genome accession: NZ_CP029660
• Coordinates: 3902334..3902789 (-)
• Length: 151 a.a.
• NCBI ID: WP_033976389.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain AR_0446
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3897334..3907789

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CSC33_RS18605	-	3897778..3898440 (+)	663	WP_023082023.1	DUF3218 family protein	-
CSC33_RS18615 (CSC33_3777)	fliP	3898723..3899475 (+)	753	WP_023082025.1	flagellar type III secretion system pore protein FliP	-
CSC33_RS18620 (CSC33_3778)	fliQ	3899520..3899789 (+)	270	WP_003083053.1	flagellar biosynthesis protein FliQ	-
CSC33_RS18625 (CSC33_3779)	fliR	3899789..3900565 (+)	777	WP_003083054.1	flagellar biosynthetic protein FliR	-
CSC33_RS18630	-	3900568..3900827 (+)	260	Protein_3620	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
CSC33_RS18635	-	3901825..3902067 (+)	243	WP_023082027.1	hypothetical protein	-
CSC33_RS18645 (CSC33_3782)	pilA	3902334..3902789 (-)	456	WP_033976389.1	pilin	Machinery gene
CSC33_RS18650 (CSC33_3783)	pilB	3903021..3904720 (+)	1700	Protein_3623	type IV-A pilus assembly ATPase PilB	-
CSC33_RS18655 (CSC33_3785)	pilC	3904724..3905944 (+)	1221	WP_110194327.1	type 4a pilus biogenesis protein PilC	Machinery gene
CSC33_RS18660 (CSC33_3786)	pilD	3905948..3906820 (+)	873	WP_033976386.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
CSC33_RS18665 (CSC33_3787)	coaE	3906817..3907428 (+)	612	WP_033970460.1	dephospho-CoA kinase	-
CSC33_RS18670 (CSC33_3788)	yacG	3907425..3907625 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 151 a.a.
• Molecular weight: 15862.20 Da
• Isoelectric Point: 7.7840

>NTDB_id=294757 CSC33_RS18645 WP_033976389.1 3902334..3902789(-) (pilA) [Pseudomonas aeruginosa strain AR_0446]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASINPLKTTVEEALSRGWSVRSGTGAEDAAKKEVPLG
VDGKANNLGTIALNPDPANGAADITLTFTMDAAGPKNKDKVITLTRTATDGLWKCTSDQEKQFIPKGCSDK

Nucleotide

• Length: 456 bp

>NTDB_id=294757 CSC33_RS18645 WP_033976389.1 3902334..3902789(-) (pilA) [Pseudomonas aeruginosa strain AR_0446]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAATACCAGAACTATGTTGCGCGCTCGGAAGGCGCTTCGGCGCTGGCGTCCATCAACCCGTTGAAGACAACTGTAG
AAGAGGCTCTTTCTCGTGGTTGGAGCGTTAGGAGTGGTACTGGTGCAGAAGACGCTGCTAAAAAAGAAGTTCCGCTGGGT
GTAGATGGAAAGGCCAATAATCTCGGCACCATTGCTCTGAACCCTGATCCTGCAAATGGTGCTGCTGATATTACTCTGAC
CTTCACCATGGATGCTGCTGGTCCGAAGAACAAAGACAAAGTCATTACTCTGACTCGTACTGCTACTGATGGCCTTTGGA
AGTGCACCAGTGATCAGGAAAAGCAGTTCATTCCGAAAGGCTGCTCTGACAAGTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-148)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	87.919	98.675	0.868
pilA	Acinetobacter baumannii strain A118	44.371	100	0.444
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	44	99.338	0.437
pilA	Vibrio cholerae strain A1552	44	99.338	0.437
pilA	Vibrio cholerae C6706	44	99.338	0.437
pilA/pilAI	Pseudomonas stutzeri DSM 10701	43.421	100	0.437
pilA/pilAII	Pseudomonas stutzeri DSM 10701	45.205	96.689	0.437
pilA	Vibrio parahaemolyticus RIMD 2210633	47.761	88.742	0.424
comP	Acinetobacter baylyi ADP1	41.333	99.338	0.411
pilA2	Legionella pneumophila str. Paris	40.136	97.351	0.391
pilA2	Legionella pneumophila strain ERS1305867	39.456	97.351	0.384
pilA/pilA1	Eikenella corrodens VA1	36.25	100	0.384
pilA	Acinetobacter nosocomialis M2	46.61	78.146	0.364