Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C1M51_RS08745 Genome accession   NZ_CP029606
Coordinates   1825958..1827094 (+) Length   378 a.a.
NCBI ID   WP_011831335.1    Uniprot ID   W7W3I9
Organism   Methylibium sp. Pch-M     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1820958..1832094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1M51_RS08720 (C1M51_08660) - 1821581..1822468 (+) 888 WP_129449123.1 branched-chain amino acid ABC transporter permease -
  C1M51_RS08725 (C1M51_08665) - 1822465..1823448 (+) 984 WP_129449125.1 branched-chain amino acid ABC transporter permease -
  C1M51_RS08730 (C1M51_08670) - 1823455..1824171 (-) 717 WP_129449127.1 YggS family pyridoxal phosphate-dependent enzyme -
  C1M51_RS08735 (C1M51_08675) pilT 1824224..1825267 (+) 1044 WP_011831333.1 type IV pilus twitching motility protein PilT Machinery gene
  C1M51_RS08740 (C1M51_08680) - 1825305..1825913 (+) 609 WP_129449129.1 cyclic nucleotide-binding domain-containing protein -
  C1M51_RS08745 (C1M51_08685) pilU 1825958..1827094 (+) 1137 WP_011831335.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C1M51_RS08750 (C1M51_08690) - 1827188..1828081 (+) 894 WP_129449131.1 NAD(P)-dependent oxidoreductase -
  C1M51_RS08755 (C1M51_08695) - 1828119..1828802 (-) 684 WP_129449133.1 BON domain-containing protein -
  C1M51_RS08760 (C1M51_08700) - 1828799..1829395 (-) 597 WP_036233929.1 phosphoheptose isomerase -
  C1M51_RS08765 (C1M51_08705) - 1829468..1829836 (-) 369 WP_129449135.1 YraN family protein -
  C1M51_RS08770 (C1M51_08710) rsmI 1829829..1830761 (+) 933 WP_242500008.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  C1M51_RS08775 (C1M51_08715) - 1830770..1831105 (-) 336 WP_129449137.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41929.09 Da        Isoelectric Point: 6.5038

>NTDB_id=294049 C1M51_RS08745 WP_011831335.1 1825958..1827094(+) (pilU) [Methylibium sp. Pch-M]
MERDQASKFVNDLLRLLVARNGSDLFLTADFPPAVKVDGRVTKVSPQPLTPQHTMALARSIMNDKQAAEFERTKECNFAI
APTGIGRFRVNAFVQQGCVGLVLRTIPQTLPTIDSLGLPQVLKDVASTKRGLVIFVGATGSGKSTSLAAMVDYRNENSYG
HIITIEDPVEFVHPHKNCIVTQREVGIDTDDWAPALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKALVSQRLLARQEGRGRVAAIEILLNTPLISDLIFKGEVAEIKEIMKKSRELG
MQTFDQSLFDLYEGQLVTYEDALRNADSVNDLRLQIKLNSNRARNSDLASGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=294049 C1M51_RS08745 WP_011831335.1 1825958..1827094(+) (pilU) [Methylibium sp. Pch-M]
ATGGAACGCGATCAAGCGTCGAAATTCGTCAACGACCTGCTGCGCCTGCTGGTCGCCCGCAACGGTTCGGATCTGTTCCT
GACGGCCGACTTTCCTCCGGCGGTCAAGGTCGACGGCCGCGTCACCAAGGTATCGCCGCAGCCGCTGACGCCGCAACACA
CGATGGCCCTGGCGCGTTCGATCATGAACGACAAGCAGGCCGCCGAGTTCGAACGCACCAAGGAGTGCAATTTCGCGATC
GCGCCGACCGGCATCGGCCGATTCCGGGTCAATGCCTTCGTGCAGCAGGGCTGCGTGGGTCTGGTGCTGCGGACGATCCC
GCAAACGCTGCCGACGATCGACTCACTCGGCCTGCCGCAGGTGCTGAAGGACGTCGCCTCGACCAAGCGCGGGCTGGTGA
TCTTCGTCGGCGCCACCGGCTCGGGCAAGAGCACCTCGTTGGCCGCGATGGTGGACTATCGCAACGAGAACTCCTACGGC
CATATCATCACCATCGAGGACCCGGTGGAGTTCGTGCACCCGCACAAGAACTGCATCGTGACGCAGCGTGAGGTCGGCAT
CGATACCGACGACTGGGCCCCGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAACACGCCGTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGCGCAGTTGCTGATGGACCTGTCGCTGAACCT
CAAGGCGCTGGTGTCGCAGCGCCTGCTGGCGCGCCAGGAAGGCCGCGGCCGCGTCGCGGCGATCGAGATCCTGTTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTGGCCGAGATCAAGGAAATCATGAAGAAGAGCCGAGAGCTCGGC
ATGCAGACCTTCGACCAGAGCCTGTTCGACCTGTACGAGGGCCAGCTAGTCACCTACGAGGATGCCCTGCGCAACGCCGA
TTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCAACCGGGCGCGCAATTCCGACCTGGCCTCGGGCACCGAGC
ACCTGACCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W7W3I9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.388

96.825

0.614
  pilU Acinetobacter baylyi ADP1

57.104

96.825

0.553
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.478

89.153

0.423
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

45.768

84.392

0.386
  pilT Acinetobacter baumannii strain A118

45.768

84.392

0.386
  pilT Legionella pneumophila strain ERS1305867

43.544

88.095

0.384
  pilT Legionella pneumophila strain Lp02

43.544

88.095

0.384
  pilT Acinetobacter baylyi ADP1

45.031

85.185

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.283

84.127

0.381
  pilT Vibrio cholerae strain A1552

45.283

84.127

0.381

Multiple sequence alignment