Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   DG474_RS00820 Genome accession   NZ_CP029257
Coordinates   159369..160310 (+) Length   313 a.a.
NCBI ID   WP_255778312.1    Uniprot ID   -
Organism   Streptococcus oralis strain CCUG 53468     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 154369..165310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DG474_RS00800 (DG474_00805) tgt 154402..155544 (+) 1143 WP_001285237.1 tRNA guanosine(34) transglycosylase Tgt -
  DG474_RS00805 (DG474_00810) - 155678..157495 (+) 1818 WP_255778311.1 acyltransferase family protein -
  DG474_RS00810 (DG474_00815) nagA 157636..158787 (+) 1152 WP_001134441.1 N-acetylglucosamine-6-phosphate deacetylase -
  DG474_RS00815 (DG474_00820) - 158941..159306 (+) 366 WP_000286399.1 DUF1033 family protein -
  DG474_RS00820 (DG474_00825) comGA/cglA/cilD 159369..160310 (+) 942 WP_255778312.1 competence type IV pilus ATPase ComGA Machinery gene
  DG474_RS00825 (DG474_00830) comGB/cglB 160258..161274 (+) 1017 WP_255778313.1 competence type IV pilus assembly protein ComGB Machinery gene
  DG474_RS00830 (DG474_00835) comGC/cglC 161276..161599 (+) 324 WP_255778314.1 competence type IV pilus major pilin ComGC Machinery gene
  DG474_RS00835 (DG474_00840) comGD/cglD 161562..161996 (+) 435 WP_255778316.1 competence type IV pilus minor pilin ComGD Machinery gene
  DG474_RS00840 (DG474_00845) comGE/cglE 161959..162261 (+) 303 WP_255778318.1 competence type IV pilus minor pilin ComGE Machinery gene
  DG474_RS00845 (DG474_00850) comGF/cglF 162224..162685 (+) 462 WP_255778319.1 competence type IV pilus minor pilin ComGF Machinery gene
  DG474_RS00850 (DG474_00855) comGG/cglG 162663..163076 (+) 414 WP_139658263.1 competence type IV pilus minor pilin ComGG Machinery gene
  DG474_RS00855 (DG474_00860) - 163109..163699 (+) 591 WP_255778320.1 class I SAM-dependent methyltransferase -
  DG474_RS00860 (DG474_00865) comYH 163758..164711 (+) 954 WP_255778321.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35526.43 Da        Isoelectric Point: 5.4353

>NTDB_id=291387 DG474_RS00820 WP_255778312.1 159369..160310(+) (comGA/cglA/cilD) [Streptococcus oralis strain CCUG 53468]
MVQEIAQKIIATAKEKKAQDIYLIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKKSSLRLSTVGDYRGYESLVIRLLHDEEQDLHFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=291387 DG474_RS00820 WP_255778312.1 159369..160310(+) (comGA/cglA/cilD) [Streptococcus oralis strain CCUG 53468]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGAAAAGAAGGCCCAGGATATCTATCTTATCCCCAAGGA
AAAGTCCTACGAGCTTCACATGCGGGTTGGAGACGAACGGTGTCTAGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAATTTGTGGCGGGTATGAACGTAGGAGAGAAGAGGCGTAGTCAGCTGGGGTCTTGCGACTATCAG
CATGGGGAGAAGAAGTCTTCTCTGCGTTTGTCTACCGTGGGAGATTATCGGGGATATGAGAGTTTGGTCATTCGTTTGTT
GCACGATGAGGAACAGGACCTGCATTTCTGGTTTCAGGATATGAACGAACTGGGTGAGCAGTACAGGCAACGGGGGCTCT
ACCTTTTTGCGGGTCCAGTTGGAAGTGGCAAGACGACTCTGATGCACGAATTAGCCAAGTCTCTGTTTAAAGGACAGCAG
GTTATGTCCATTGAAGATCCTGTCGAGATCAAGCAGGACGACATGCTCCAGTTGCAGTTGAATGAGGCGATTGGATTGAC
CTATGAAAATCTGATCAAACTGTCTCTTCGGCATCGTCCTGACCTCTTGATTATCGGTGAAATTCGTGATAGCGAGACAG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGGGCGACGGTCTTTTCAACCATTCATGCCAAGAGTATCCGAGGTGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAACTAGCAGTTGTCCTGCAAGGAGTCTGCTACCAGAGATTAATTGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAGCAGATTGATCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

89.457

100

0.895
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

88.498

100

0.885
  comGA/cglA/cilD Streptococcus pneumoniae D39

88.498

100

0.885
  comGA/cglA/cilD Streptococcus pneumoniae R6

88.498

100

0.885
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

88.498

100

0.885
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.881

99.361

0.665
  comYA Streptococcus mutans UA140

66.881

99.361

0.665
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.29

99.042

0.607
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

43.346

84.026

0.364

Multiple sequence alignment