Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   DG474_RS00825 Genome accession   NZ_CP029257
Coordinates   160258..161274 (+) Length   338 a.a.
NCBI ID   WP_255778313.1    Uniprot ID   -
Organism   Streptococcus oralis strain CCUG 53468     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 155258..166274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DG474_RS00805 (DG474_00810) - 155678..157495 (+) 1818 WP_255778311.1 acyltransferase family protein -
  DG474_RS00810 (DG474_00815) nagA 157636..158787 (+) 1152 WP_001134441.1 N-acetylglucosamine-6-phosphate deacetylase -
  DG474_RS00815 (DG474_00820) - 158941..159306 (+) 366 WP_000286399.1 DUF1033 family protein -
  DG474_RS00820 (DG474_00825) comGA/cglA/cilD 159369..160310 (+) 942 WP_255778312.1 competence type IV pilus ATPase ComGA Machinery gene
  DG474_RS00825 (DG474_00830) comGB/cglB 160258..161274 (+) 1017 WP_255778313.1 competence type IV pilus assembly protein ComGB Machinery gene
  DG474_RS00830 (DG474_00835) comGC/cglC 161276..161599 (+) 324 WP_255778314.1 competence type IV pilus major pilin ComGC Machinery gene
  DG474_RS00835 (DG474_00840) comGD/cglD 161562..161996 (+) 435 WP_255778316.1 competence type IV pilus minor pilin ComGD Machinery gene
  DG474_RS00840 (DG474_00845) comGE/cglE 161959..162261 (+) 303 WP_255778318.1 competence type IV pilus minor pilin ComGE Machinery gene
  DG474_RS00845 (DG474_00850) comGF/cglF 162224..162685 (+) 462 WP_255778319.1 competence type IV pilus minor pilin ComGF Machinery gene
  DG474_RS00850 (DG474_00855) comGG/cglG 162663..163076 (+) 414 WP_139658263.1 competence type IV pilus minor pilin ComGG Machinery gene
  DG474_RS00855 (DG474_00860) - 163109..163699 (+) 591 WP_255778320.1 class I SAM-dependent methyltransferase -
  DG474_RS00860 (DG474_00865) comYH 163758..164711 (+) 954 WP_255778321.1 class I SAM-dependent methyltransferase Machinery gene
  DG474_RS00865 (DG474_00870) - 164762..165952 (+) 1191 WP_000167792.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38604.76 Da        Isoelectric Point: 9.5359

>NTDB_id=291388 DG474_RS00825 WP_255778313.1 160258..161274(+) (comGB/cglB) [Streptococcus oralis strain CCUG 53468]
MDISQVFRLKRKKLATAKQKKIITLFNNLFSSGFHLVEIISFLGRSALLEKDYVAQMHQGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLSKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGLVLVCSLSLLLALTFYKRSSKMRVFSMLARIPFLGIFVQTYLTAYYAREWGNMISQGMELMQIFQIMQEQGSQLFKEI
GQDLAQALQNGREFSQTIATYPYFKKELSLIIEYGEVKSKLGSELEIYAEKTWEVFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=291388 DG474_RS00825 WP_255778313.1 160258..161274(+) (comGB/cglB) [Streptococcus oralis strain CCUG 53468]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAGAAGAAAATCATTACCTTGTTTAA
CAATCTCTTCTCCAGTGGCTTTCATTTGGTGGAAATTATTTCTTTCTTGGGCAGAAGTGCTTTGCTGGAAAAGGACTATG
TGGCCCAGATGCACCAAGGCTTGTCTCAGGGGAAATCCTTCTCAGAAATGATGGAAAGTTTGGGCTTTTCAAGTGCTATT
GTGACTCAATTATCTCTAGCTGAAGTTCATGGAAATCTCCATCTGAGTCTGGGGAAGATAGAAGAATATCTGGATAATTT
GTCCAAGGTCAAGAAGAAGTTAATTGAAGTAGCGACTTATCCCCTGATTTTGCTGGGATTTCTCCTGCTAATCATGTTGG
GGCTCAGGAATTATTTGCTCCCCCAACTGGACAGTAGCAATATCGCCACTCAAATCATCGGCAATCTGCCACAAATATTT
CTGGGACTAGTGTTGGTTTGCTCTCTATCTTTACTTTTAGCCCTCACTTTTTACAAAAGAAGTTCCAAGATGCGGGTCTT
CTCGATGTTAGCGCGGATTCCCTTTCTAGGAATCTTTGTTCAGACCTATCTGACGGCCTATTACGCGCGTGAATGGGGCA
ATATGATTTCACAGGGGATGGAGCTGATGCAGATTTTTCAGATCATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATT
GGTCAAGATCTGGCTCAAGCCCTGCAAAATGGTCGGGAATTTTCTCAAACCATAGCAACCTATCCTTACTTTAAAAAGGA
GTTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGGAGCGAGTTGGAAATCTATGCTGAGAAAACTTGGG
AGGTATTTTTTACCCGAGTCAACCGCACTATGAACTTAGTACAGCCACTGGTTTTTATTTTTGTGGCCCTGATTATCGTT
TTACTTTATGCGGCAATGCTTATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

93.195

100

0.932
  comGB/cglB Streptococcus mitis NCTC 12261

92.308

100

0.923
  comGB/cglB Streptococcus pneumoniae Rx1

90.533

100

0.905
  comGB/cglB Streptococcus pneumoniae D39

90.533

100

0.905
  comGB/cglB Streptococcus pneumoniae R6

90.533

100

0.905
  comGB/cglB Streptococcus pneumoniae TIGR4

90.533

100

0.905
  comYB Streptococcus gordonii str. Challis substr. CH1

73.214

99.408

0.728
  comYB Streptococcus mutans UA140

58.934

94.379

0.556
  comYB Streptococcus mutans UA159

58.934

94.379

0.556
  comGB Lactococcus lactis subsp. cremoris KW2

52.367

100

0.524

Multiple sequence alignment