Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CCV52592_RS02465 Genome accession   NC_009715
Coordinates   509673..510794 (+) Length   373 a.a.
NCBI ID   WP_011991975.1    Uniprot ID   A7GX44
Organism   Campylobacter curvus 525.92     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504673..515794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCV52592_RS02430 (CCV52592_0992) - 505502..506137 (+) 636 WP_011991970.1 DUF6803 family protein -
  CCV52592_RS02435 (CCV52592_0991) hisG 506131..506751 (-) 621 WP_011991971.1 ATP phosphoribosyltransferase -
  CCV52592_RS02440 (CCV52592_0993) - 506748..507377 (-) 630 WP_011991972.1 type III pantothenate kinase -
  CCV52592_RS02445 (CCV52592_0994) - 507364..507696 (-) 333 WP_041743215.1 hypothetical protein -
  CCV52592_RS02450 (CCV52592_0995) - 507696..508700 (-) 1005 WP_011991973.1 L-seryl-tRNA selenium transferase -
  CCV52592_RS02455 (CCV52592_0996) - 508700..509263 (-) 564 WP_011991974.1 hypothetical protein -
  CCV52592_RS02460 (CCV52592_0997) gatC 509382..509669 (+) 288 WP_009650004.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CCV52592_RS02465 (CCV52592_0998) pilT 509673..510794 (+) 1122 WP_011991975.1 type IV pilus twitching motility protein PilT Machinery gene
  CCV52592_RS02470 (CCV52592_0999) - 510796..511413 (+) 618 WP_009650144.1 CvpA family protein -
  CCV52592_RS02475 (CCV52592_2147) - 511410..511877 (+) 468 WP_009650275.1 Fur family transcriptional regulator -
  CCV52592_RS02480 (CCV52592_1349) lysS 511882..513390 (+) 1509 WP_231931873.1 lysine--tRNA ligase -
  CCV52592_RS02485 (CCV52592_1350) - 513387..514631 (+) 1245 WP_011991977.1 serine hydroxymethyltransferase -
  CCV52592_RS02490 (CCV52592_1351) - 514631..515173 (+) 543 WP_011991978.1 DUF1882 domain-containing protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41363.45 Da        Isoelectric Point: 7.2082

>NTDB_id=28807 CCV52592_RS02465 WP_011991975.1 509673..510794(+) (pilT) [Campylobacter curvus 525.92]
MDLIQTNNNIVQAPPRPTNTDIQTLLKTVVFNKASDLHLVARSEPQIRIDGTLRPLELGILSGKDIETLCYALITDAQKS
ELENNKELDFAIELPSIGRFRGNYYYTMNGDLAAAFRIIPIEIPSLDELKSPQIFKDIIKREKGLILVTGPTGSGKSTTL
AAMLNEINLNYRKHIITIEDPVEFVHSNKKALFSHRNIGTDTHSYARALKFSLREDPDIILVGEMRDKETISIAITAAET
GHLVFGTLHTNSAIQTINRIVDSFDGSEQLQVRNMLSVSLTAVISQSLLPKTNGGRCAIHEILINNMAVANLIRENKVHQ
IYSQMQLNQQQTGMCTQTQSLIKAIRGGLITKENALRYSTSQQELLNTMGAGA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=28807 CCV52592_RS02465 WP_011991975.1 509673..510794(+) (pilT) [Campylobacter curvus 525.92]
ATGGATCTGATACAAACGAACAATAACATAGTCCAAGCACCTCCAAGACCGACAAATACAGACATACAAACATTACTAAA
AACCGTTGTTTTTAATAAAGCCAGCGACTTGCATCTTGTCGCAAGATCCGAGCCTCAAATCAGGATCGACGGCACATTGC
GTCCTCTCGAGCTTGGAATTTTAAGCGGCAAGGACATAGAGACTTTGTGTTACGCACTGATAACGGATGCTCAAAAAAGC
GAGCTTGAAAATAACAAAGAACTTGACTTTGCGATAGAGCTACCAAGTATCGGACGCTTTCGCGGCAACTACTACTACAC
CATGAACGGCGATCTAGCGGCTGCATTTCGTATCATACCTATCGAAATTCCATCTCTTGATGAGCTGAAATCTCCTCAGA
TTTTTAAAGACATCATAAAGCGCGAAAAGGGGCTTATTTTAGTCACCGGGCCTACTGGTAGCGGTAAATCTACCACCCTT
GCAGCGATGCTAAACGAGATAAATTTAAACTATCGAAAGCATATCATAACGATAGAAGACCCGGTGGAATTCGTCCATAG
CAACAAAAAAGCGCTGTTTTCACACAGAAATATCGGCACTGATACGCACTCATACGCTAGAGCGCTTAAATTTTCACTGC
GCGAAGACCCTGACATCATCCTGGTGGGCGAGATGAGAGATAAAGAGACGATATCTATCGCGATAACAGCGGCGGAGACC
GGACACCTAGTTTTTGGAACTTTGCATACGAATTCCGCTATCCAGACGATAAACAGGATAGTCGACAGCTTTGACGGCAG
CGAGCAGCTTCAGGTGCGAAACATGCTCAGCGTCTCGCTAACGGCGGTCATCTCGCAAAGCCTGCTTCCAAAGACCAACG
GCGGCAGGTGCGCGATACATGAAATTTTGATCAACAACATGGCCGTTGCAAATTTGATCCGTGAAAACAAGGTGCATCAG
ATCTACTCTCAGATGCAGCTAAATCAGCAGCAAACTGGCATGTGCACGCAGACGCAGTCTTTGATAAAGGCCATAAGAGG
CGGCCTTATCACTAAAGAAAATGCATTGCGTTATTCGACCAGTCAGCAAGAGCTTTTAAATACTATGGGTGCAGGTGCTT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7GX44

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

52.754

92.493

0.488
  pilT Neisseria meningitidis 8013

52.464

92.493

0.485
  pilT Acinetobacter nosocomialis M2

52.616

92.225

0.485
  pilT Acinetobacter baumannii D1279779

52.616

92.225

0.485
  pilT Acinetobacter baumannii strain A118

52.616

92.225

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

88.472

0.48
  pilT Vibrio cholerae strain A1552

54.242

88.472

0.48
  pilT Legionella pneumophila strain Lp02

51.94

89.812

0.466
  pilT Legionella pneumophila strain ERS1305867

51.94

89.812

0.466
  pilT Acinetobacter baylyi ADP1

52.424

88.472

0.464
  pilT Pseudomonas aeruginosa PAK

50.746

89.812

0.456
  pilT Pseudomonas stutzeri DSM 10701

49.558

90.885

0.45
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.011

96.247

0.424

Multiple sequence alignment