Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   SK637_RS01010 Genome accession   NZ_CP028415
Coordinates   207131..208072 (+) Length   313 a.a.
NCBI ID   WP_033688033.1    Uniprot ID   -
Organism   Streptococcus mitis strain SK637     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 202131..213072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK637_RS00990 (SK637_00197) - 202203..204020 (+) 1818 WP_033688029.1 acyltransferase family protein -
  SK637_RS00995 (SK637_00198) nagA 204172..205323 (+) 1152 WP_033688030.1 N-acetylglucosamine-6-phosphate deacetylase -
  SK637_RS01000 (SK637_00199) - 205486..206544 (+) 1059 WP_033688031.1 zinc-dependent alcohol dehydrogenase family protein -
  SK637_RS01005 (SK637_00200) - 206690..207055 (+) 366 WP_033688032.1 DUF1033 family protein -
  SK637_RS01010 (SK637_00201) comGA/cglA/cilD 207131..208072 (+) 942 WP_033688033.1 competence type IV pilus ATPase ComGA Machinery gene
  SK637_RS01015 (SK637_00202) comGB/cglB 208020..209036 (+) 1017 WP_080710441.1 competence type IV pilus assembly protein ComGB Machinery gene
  SK637_RS01020 (SK637_00203) comGC/cglC 209038..209364 (+) 327 WP_033688035.1 competence type IV pilus major pilin ComGC Machinery gene
  SK637_RS01025 (SK637_00204) comGD/cglD 209357..209761 (+) 405 WP_000588010.1 competence type IV pilus minor pilin ComGD Machinery gene
  SK637_RS01030 (SK637_00205) comGE/cglE 209724..210026 (+) 303 WP_272898261.1 competence type IV pilus minor pilin ComGE Machinery gene
  SK637_RS01035 (SK637_00206) comGF/cglF 209989..210450 (+) 462 WP_033688037.1 competence type IV pilus minor pilin ComGF Machinery gene
  SK637_RS01040 (SK637_00207) comGG/cglG 210428..210838 (+) 411 WP_033688038.1 competence type IV pilus minor pilin ComGG Machinery gene
  SK637_RS01045 (SK637_00208) - 210871..211458 (+) 588 WP_033688039.1 class I SAM-dependent methyltransferase -
  SK637_RS01050 (SK637_00209) comYH 211519..212472 (+) 954 WP_033688040.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35602.64 Da        Isoelectric Point: 6.2413

>NTDB_id=284673 SK637_RS01010 WP_033688033.1 207131..208072(+) (comGA/cglA/cilD) [Streptococcus mitis strain SK637]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDKRCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGQESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFTNKDYQEHKPTSWNEQIDQLLKDGHITSFQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=284673 SK637_RS01010 WP_033688033.1 207131..208072(+) (comGA/cglA/cilD) [Streptococcus mitis strain SK637]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCCCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGACGCCTATGAACTTCATATGAGGGTAGGAGACAAGCGCTGTAAAATCGGTTGTTATGATTTCGAAAAATTTGCGGCCG
TCATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTTGGAGAAAAGCGACGTAGTCAACTTGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCCCTACGTCTATCTACTGTAGGCGATTATCGAGGGCAGGAGAGTTTGGTGATTCGCTTGTT
GCATGATGAGGAGCAGGATTTGCATTTTTGGTTTCAGGATATTGAAGAACTTGGCAAGCAGTATAGGCAACGTGGTCTCT
ATCTCTTTGCTGGTCCAGTCGGAAGTGGCAAGACAACCCTGATGCATGAATTGGCTAAATCTCTCTTTAAAGGACAGCAA
GTCATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGATGACATGCTCCAGTTGCAGTTGAATGAGGCAATCGGACTAAC
CTATGAAAATCTGATTAAGCTTTCCCTACGGCATCGTCCTGATCTCTTGATTATCGGAGAAATTCGGGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATCCGAGGTGTTTAT
GAGCGCCTGCTGGAGTTGGGTGTGAGTGAGGAGGAATTAGCAGTTGTTCTGCAAGGAGTTTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTACAAACAAAGACTATCAAGAACACAAGCCAACTAGCTGGAATGAACAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTTTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.125

100

0.971
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.208

100

0.952
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.208

100

0.952
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.208

100

0.952
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.208

100

0.952
  comYA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comYA Streptococcus mutans UA159

64.952

99.361

0.645
  comYA Streptococcus mutans UA140

64.952

99.361

0.645
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.258

99.042

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.993

89.776

0.377

Multiple sequence alignment