Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SK637_RS01015 Genome accession   NZ_CP028415
Coordinates   208020..209036 (+) Length   338 a.a.
NCBI ID   WP_080710441.1    Uniprot ID   -
Organism   Streptococcus mitis strain SK637     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 203020..214036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK637_RS00995 (SK637_00198) nagA 204172..205323 (+) 1152 WP_033688030.1 N-acetylglucosamine-6-phosphate deacetylase -
  SK637_RS01000 (SK637_00199) - 205486..206544 (+) 1059 WP_033688031.1 zinc-dependent alcohol dehydrogenase family protein -
  SK637_RS01005 (SK637_00200) - 206690..207055 (+) 366 WP_033688032.1 DUF1033 family protein -
  SK637_RS01010 (SK637_00201) comGA/cglA/cilD 207131..208072 (+) 942 WP_033688033.1 competence type IV pilus ATPase ComGA Machinery gene
  SK637_RS01015 (SK637_00202) comGB/cglB 208020..209036 (+) 1017 WP_080710441.1 competence type IV pilus assembly protein ComGB Machinery gene
  SK637_RS01020 (SK637_00203) comGC/cglC 209038..209364 (+) 327 WP_033688035.1 competence type IV pilus major pilin ComGC Machinery gene
  SK637_RS01025 (SK637_00204) comGD/cglD 209357..209761 (+) 405 WP_000588010.1 competence type IV pilus minor pilin ComGD Machinery gene
  SK637_RS01030 (SK637_00205) comGE/cglE 209724..210026 (+) 303 WP_272898261.1 competence type IV pilus minor pilin ComGE Machinery gene
  SK637_RS01035 (SK637_00206) comGF/cglF 209989..210450 (+) 462 WP_033688037.1 competence type IV pilus minor pilin ComGF Machinery gene
  SK637_RS01040 (SK637_00207) comGG/cglG 210428..210838 (+) 411 WP_033688038.1 competence type IV pilus minor pilin ComGG Machinery gene
  SK637_RS01045 (SK637_00208) - 210871..211458 (+) 588 WP_033688039.1 class I SAM-dependent methyltransferase -
  SK637_RS01050 (SK637_00209) comYH 211519..212472 (+) 954 WP_033688040.1 class I SAM-dependent methyltransferase Machinery gene
  SK637_RS01055 (SK637_00210) - 212522..213712 (+) 1191 WP_033688041.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38639.63 Da        Isoelectric Point: 9.5998

>NTDB_id=284674 SK637_RS01015 WP_080710441.1 208020..209036(+) (comGB/cglB) [Streptococcus mitis strain SK637]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGFSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMIVFVSVGVLLALTFYKRSSKMRIFSILARFPFFGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQALQNGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=284674 SK637_RS01015 WP_080710441.1 208020..209036(+) (comGB/cglB) [Streptococcus mitis strain SK637]
ATGGACATATCACAAGTTTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
CAATCTCTTTTCCAGTGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAACAGTGTG
TGACCCAGATGCGCACAGGCTTGTCTCAGGGAAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGTACCATT
GTCACTCAGTTATCCCTAGCGGAAGTCCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
TGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCCTTGATTTTACTGGGATTTCTTCTCTTAATCATGCTGG
GACTACGGAACTACCTACTACCACAACTGGATAGTAGCAATATTGCCACCCAAATCATTGGCAATCTGCCACAAATCTTT
CTAGGCATGATAGTTTTTGTTTCAGTAGGTGTCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCGCATCTT
TTCTATCCTAGCACGCTTTCCCTTTTTTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTACGCGCGTGAATGGGGGA
ATATGATTTCGCAGGGGATGGAACTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATTTGGCGCAAGCCCTACAAAATGGCCGCGAATTTTCTCAAACCATAGCAACTTATCCTTTCTTTAAGAAGGA
GTTGAGTCTCATCATCGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AGGCCTTTTTTACCCGAGTCAATCGCACCATGAATCTGGTGCAGCCACTGGTTTTTATCTTTGTGGCTCTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis NCTC 12261

95.858

100

0.959
  comGB/cglB Streptococcus mitis SK321

95.562

100

0.956
  comGB/cglB Streptococcus pneumoniae D39

94.379

100

0.944
  comGB/cglB Streptococcus pneumoniae R6

94.379

100

0.944
  comGB/cglB Streptococcus pneumoniae TIGR4

94.379

100

0.944
  comGB/cglB Streptococcus pneumoniae Rx1

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

71.429

99.408

0.71
  comYB Streptococcus mutans UA140

58.621

94.379

0.553
  comYB Streptococcus mutans UA159

58.621

94.379

0.553
  comGB Lactococcus lactis subsp. cremoris KW2

50.599

98.817

0.5

Multiple sequence alignment