Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   C7S18_RS18970 Genome accession   NZ_CP027860
Coordinates   4919997..4920428 (-) Length   143 a.a.
NCBI ID   WP_106893040.1    Uniprot ID   A0A2P1PW86
Organism   Ahniella affigens strain D13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4914997..4925428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7S18_RS18950 (C7S18_18940) - 4916251..4917513 (-) 1263 WP_106893037.1 DUF6596 domain-containing protein -
  C7S18_RS18955 (C7S18_18945) - 4917570..4917932 (-) 363 WP_106894107.1 YciI family protein -
  C7S18_RS18960 (C7S18_18950) - 4918150..4918722 (+) 573 WP_106893038.1 Pycsar system effector family protein -
  C7S18_RS18965 (C7S18_18955) - 4919145..4919588 (-) 444 WP_106893039.1 pilin -
  C7S18_RS18970 (C7S18_18960) pilE 4919997..4920428 (-) 432 WP_106893040.1 pilin Machinery gene
  C7S18_RS18975 (C7S18_18965) - 4920499..4921218 (-) 720 WP_106893041.1 hypothetical protein -
  C7S18_RS18980 (C7S18_18970) pilR 4921221..4922618 (-) 1398 WP_106893042.1 sigma-54 dependent transcriptional regulator Regulator
  C7S18_RS18985 (C7S18_18975) - 4922735..4924360 (-) 1626 WP_170113359.1 ATP-binding protein -
  C7S18_RS18990 (C7S18_18980) - 4924537..4925316 (-) 780 WP_106893044.1 AMP nucleosidase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14813.87 Da        Isoelectric Point: 4.4200

>NTDB_id=281053 C7S18_RS18970 WP_106893040.1 4919997..4920428(-) (pilE) [Ahniella affigens strain D13]
MKKTQGFTLIELMIVVAIIAILAAIALPAYQDYTIRAQVSECPTLAAGAKTAVAETFQNTGTFPTSNTEAGMAAATEIVG
NYVTQVEVSGAGVITCTFGNDANTLIAGQNMTLTPDGTTNVGSITWQCGGSIDAKYRPSSCRP

Nucleotide


Download         Length: 432 bp        

>NTDB_id=281053 C7S18_RS18970 WP_106893040.1 4919997..4920428(-) (pilE) [Ahniella affigens strain D13]
ATGAAGAAGACCCAAGGTTTTACCCTGATCGAGCTGATGATCGTTGTCGCCATTATCGCCATTCTGGCCGCGATTGCTCT
GCCGGCTTATCAGGACTACACGATCCGCGCTCAAGTTTCTGAATGCCCGACGCTGGCTGCTGGCGCCAAGACCGCCGTTG
CCGAAACCTTCCAGAACACCGGTACGTTCCCGACCTCGAACACGGAAGCCGGCATGGCCGCTGCGACGGAAATCGTGGGT
AACTATGTGACGCAAGTCGAAGTTAGCGGCGCAGGCGTCATCACCTGCACGTTCGGTAACGACGCGAACACGCTGATCGC
TGGCCAGAACATGACGCTGACCCCGGACGGCACCACGAACGTCGGCAGCATCACGTGGCAATGCGGTGGCTCGATCGATG
CCAAGTACCGCCCAAGCAGCTGCCGTCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2P1PW86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

47.771

100

0.524
  pilE Neisseria gonorrhoeae strain FA1090

46.154

100

0.503
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.368

100

0.496
  pilA2 Legionella pneumophila str. Paris

49.296

99.301

0.49
  pilA2 Legionella pneumophila strain ERS1305867

49.296

99.301

0.49
  comP Acinetobacter baylyi ADP1

44.371

100

0.469
  pilA/pilA1 Eikenella corrodens VA1

42.675

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

38.554

100

0.448
  pilA Acinetobacter baumannii strain A118

37.838

100

0.392
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.889

100

0.392
  pilA Pseudomonas aeruginosa PAK

35.526

100

0.378
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.41

97.203

0.364
  pilA Haemophilus influenzae Rd KW20

37.41

97.203

0.364

Multiple sequence alignment