Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PNAP_RS00395 Genome accession   NC_008781
Coordinates   80771..81925 (-) Length   384 a.a.
NCBI ID   WP_011799513.1    Uniprot ID   A1VIC5
Organism   Polaromonas naphthalenivorans CJ2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 75771..86925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNAP_RS00380 (Pnap_0074) - 77560..78861 (+) 1302 WP_011799510.1 hypothetical protein -
  PNAP_RS00385 (Pnap_0075) - 78981..79361 (+) 381 WP_198140661.1 hypothetical protein -
  PNAP_RS00390 (Pnap_0076) - 79407..80693 (-) 1287 WP_011799512.1 solute carrier family 23 protein -
  PNAP_RS00395 (Pnap_0077) pilU 80771..81925 (-) 1155 WP_011799513.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PNAP_RS24815 (Pnap_0078) - 82167..82568 (+) 402 WP_049763615.1 helix-turn-helix domain-containing protein -
  PNAP_RS24820 (Pnap_0079) - 82598..83347 (+) 750 WP_232290778.1 transposase -
  PNAP_RS00405 (Pnap_0080) - 83455..84699 (+) 1245 WP_011799516.1 efflux RND transporter periplasmic adaptor subunit -
  PNAP_RS00410 (Pnap_0081) - 84719..85981 (+) 1263 WP_011799517.1 FtsX-like permease family protein -
  PNAP_RS00415 (Pnap_0082) - 86106..86903 (-) 798 WP_011799518.1 hypothetical protein -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42302.77 Da        Isoelectric Point: 6.7255

>NTDB_id=27266 PNAP_RS00395 WP_011799513.1 80771..81925(-) (pilU) [Polaromonas naphthalenivorans CJ2]
MPLPAQAAPSPSPSPSDPDDLGRYLALMVAHGASDLFLSAEVAASLKIQGRIRRLPEPPLTGTHIKRLAYSVMRDSQVRE
FEADLECDLAIAMDALGRFRVNVFRQRGEVAVVVRHINSTIPTLESLKLPPVLKTLAMLKRGLVLVVGAAGSGKSTTLAA
MLDYRNNMASDHILTVEDPMEFEHSHKKCIVDQREVGLDTRSFDEALRRAMREAPDVIMIGEIRDRETMQHAMAYAETGH
LCVSTLHANNANQAIQRVLNFFPETAQRQVLMDLSLNLKGVVGQRLLAGAAGKRVPAVEVMLLSPYVADLIQKGQTDEIK
AVMAKSVELGMATFDQSLYELYVRGDITHEQAIEHADSKTDLSLRIRLASGRRPDTEALQVLRD

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=27266 PNAP_RS00395 WP_011799513.1 80771..81925(-) (pilU) [Polaromonas naphthalenivorans CJ2]
ATGCCACTGCCCGCCCAAGCCGCCCCATCCCCGTCCCCATCCCCGTCCGATCCCGATGACCTCGGGCGCTACCTGGCGCT
GATGGTCGCCCACGGCGCATCCGACCTTTTCCTGTCGGCTGAGGTGGCCGCCTCGCTCAAGATCCAGGGCCGCATCCGCC
GCCTGCCCGAGCCGCCGCTGACGGGAACCCACATCAAGCGCCTGGCGTATTCGGTGATGCGCGACAGCCAGGTTCGCGAG
TTCGAGGCCGATCTGGAGTGCGACCTGGCGATTGCCATGGATGCGCTGGGCCGCTTTCGCGTCAATGTGTTCCGCCAGCG
CGGCGAGGTGGCGGTGGTGGTGCGGCACATCAACAGCACGATCCCGACGCTCGAATCGCTGAAGCTGCCGCCGGTGCTGA
AAACGCTGGCCATGCTCAAGCGCGGCCTGGTGCTGGTGGTCGGCGCGGCCGGCTCGGGCAAATCGACCACGCTGGCGGCC
ATGCTGGACTACCGCAACAACATGGCCAGCGACCACATCCTGACGGTGGAAGACCCGATGGAGTTCGAGCACAGCCATAA
GAAATGCATCGTTGATCAGCGCGAGGTCGGGCTGGACACGCGCTCTTTCGACGAAGCGCTGCGCCGCGCCATGCGCGAAG
CGCCCGACGTGATCATGATCGGTGAAATCCGCGACCGCGAAACCATGCAGCACGCCATGGCCTATGCCGAAACCGGCCAC
CTGTGCGTCTCGACCCTGCATGCCAACAATGCCAACCAGGCCATCCAGCGCGTGCTGAATTTTTTCCCGGAAACCGCGCA
GCGCCAGGTGCTGATGGACCTGTCGCTCAACCTCAAGGGCGTCGTCGGCCAGCGCCTGCTGGCAGGCGCGGCCGGCAAAC
GGGTTCCGGCGGTCGAGGTGATGCTGCTGTCGCCCTATGTCGCCGATTTGATCCAGAAGGGCCAGACCGACGAGATCAAG
GCGGTGATGGCCAAGAGCGTCGAGCTGGGCATGGCCACCTTCGACCAGTCGCTGTACGAGCTGTACGTGCGCGGCGACAT
CACGCACGAGCAGGCCATCGAGCATGCCGACTCGAAAACCGACCTGTCGCTGCGCATCCGGCTGGCGTCCGGGCGCCGCC
CCGACACCGAAGCCCTGCAGGTGCTGCGCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(30-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1VIC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.728

90.885

0.497
  pilU Vibrio cholerae strain A1552

50.685

95.052

0.482
  pilU Acinetobacter baylyi ADP1

48.58

91.667

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.97

88.021

0.396
  pilT Legionella pneumophila strain Lp02

43.402

88.802

0.385
  pilT Legionella pneumophila strain ERS1305867

43.402

88.802

0.385
  pilT Pseudomonas aeruginosa PAK

43.917

87.76

0.385
  pilT Acinetobacter baumannii strain A118

44.444

86.719

0.385
  pilT Acinetobacter baumannii D1279779

44.444

86.719

0.385
  pilT Acinetobacter nosocomialis M2

44.444

86.719

0.385
  pilT Acinetobacter baylyi ADP1

44.681

85.677

0.383
  pilT Vibrio cholerae strain A1552

42.197

90.104

0.38
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.197

90.104

0.38
  pilT Pseudomonas stutzeri DSM 10701

42.73

87.76

0.375

Multiple sequence alignment