Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PPRO_RS02630 Genome accession   NC_008609
Coordinates   574099..575175 (+) Length   358 a.a.
NCBI ID   WP_011734480.1    Uniprot ID   A1ALE7
Organism   Pelobacter propionicus DSM 2379     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 569099..580175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPRO_RS02615 (Ppro_0533) - 569129..570802 (+) 1674 WP_011734477.1 glycosyltransferase family 39 protein -
  PPRO_RS02620 (Ppro_0534) dnaB 570917..572293 (+) 1377 WP_011734478.1 replicative DNA helicase -
  PPRO_RS02625 (Ppro_0535) - 572384..574036 (+) 1653 WP_011734479.1 GspE/PulE family protein -
  PPRO_RS02630 (Ppro_0536) pilT 574099..575175 (+) 1077 WP_011734480.1 type IV pilus twitching motility protein PilT Machinery gene
  PPRO_RS02635 (Ppro_0537) moaC 575211..575693 (+) 483 WP_011734481.1 cyclic pyranopterin monophosphate synthase MoaC -
  PPRO_RS02640 (Ppro_0538) - 575690..576187 (+) 498 WP_011734482.1 molybdenum cofactor biosynthesis protein B -
  PPRO_RS02645 (Ppro_0539) - 576551..577726 (+) 1176 WP_011734483.1 AAA family ATPase -
  PPRO_RS02650 (Ppro_0540) - 577719..578429 (+) 711 WP_011734484.1 hypothetical protein -
  PPRO_RS02655 (Ppro_0541) - 578500..578694 (+) 195 Protein_544 molybdenum cofactor biosynthesis protein B -
  PPRO_RS02660 (Ppro_0542) - 578891..579748 (+) 858 WP_011734486.1 GGDEF domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39867.05 Da        Isoelectric Point: 7.3211

>NTDB_id=27004 PPRO_RS02630 WP_011734480.1 574099..575175(+) (pilT) [Pelobacter propionicus DSM 2379]
MARIDALFRMMKEQGASDLHLSTGNPPIFRLHGEMVRLNFKSLGHDELHSILFEILNAKQKEQFETTKDLDFAYAVPDLA
RFRGNILMQHRGIAAVFRIIPGKVLSADDLNLPDGVRRMTNFKKGLVLVTGPTGSGKSTTLAALIDHINSTRKEHILTLE
DPLEFIHENKQSLLNQRQIGEHTQSFSAALKAALREDPDIILVGEMRDLETIQLAMSAAETGHLVFGTLHTNTAAKTIDR
IIDVFPTEQQQQVRAMLSESLRGVVCQQLLKTAEGKGRVAAFEIMLGTPAIGNLIREAKTFQIPSIIQTAKKDGMQLMDQ
HLLDLLKTKKVNPEEACRCAIDKKQFEQYLPADSAKAD

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=27004 PPRO_RS02630 WP_011734480.1 574099..575175(+) (pilT) [Pelobacter propionicus DSM 2379]
ATGGCACGTATTGACGCACTTTTCAGGATGATGAAAGAGCAGGGGGCATCGGACCTGCACCTCTCCACCGGCAATCCGCC
CATTTTTCGCCTGCATGGCGAGATGGTCCGGCTCAACTTCAAATCCCTGGGGCACGACGAGCTGCACTCGATCCTGTTCG
AGATCTTGAATGCCAAGCAGAAGGAGCAGTTCGAGACGACCAAGGATCTGGACTTCGCCTATGCCGTTCCGGACCTGGCG
CGCTTCCGGGGCAACATCCTCATGCAGCACCGGGGCATCGCCGCGGTGTTCCGCATCATACCGGGCAAGGTCCTCTCCGC
CGACGACCTGAACCTGCCCGACGGGGTGCGGCGCATGACCAATTTCAAGAAGGGGCTGGTGCTGGTCACCGGCCCCACCG
GCTCGGGCAAATCCACCACCCTGGCCGCCCTGATCGACCACATCAACTCCACCCGCAAGGAGCATATCCTCACCCTGGAG
GACCCGCTGGAGTTCATCCACGAGAACAAGCAGTCGCTGCTCAACCAGCGCCAGATCGGCGAGCACACCCAGAGCTTCTC
CGCAGCCCTCAAGGCGGCCCTGCGGGAGGATCCGGACATCATCCTGGTGGGGGAGATGCGCGACCTGGAGACCATTCAGC
TGGCCATGAGCGCCGCCGAGACCGGACACCTGGTGTTCGGCACCCTGCACACCAACACGGCAGCCAAGACCATCGACCGG
ATCATCGACGTCTTTCCCACCGAGCAGCAGCAGCAGGTGCGGGCCATGTTGTCCGAGTCGCTCAGGGGGGTGGTCTGCCA
GCAGCTGCTCAAGACCGCCGAGGGGAAGGGGCGCGTGGCCGCCTTCGAAATCATGCTCGGCACCCCTGCCATCGGCAACC
TGATCCGCGAGGCCAAGACCTTCCAGATCCCCTCCATCATCCAGACCGCCAAGAAGGACGGCATGCAGCTCATGGACCAG
CATCTGCTGGACCTGCTCAAGACCAAGAAGGTCAACCCGGAAGAGGCCTGCCGCTGCGCCATCGACAAGAAGCAGTTCGA
ACAGTACCTGCCGGCTGACAGCGCCAAGGCGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1ALE7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

55.043

96.927

0.534
  pilT Acinetobacter baumannii D1279779

55.043

96.927

0.534
  pilT Acinetobacter baumannii strain A118

55.043

96.927

0.534
  pilT Pseudomonas stutzeri DSM 10701

55.394

95.81

0.531
  pilT Legionella pneumophila strain Lp02

57.1

92.458

0.528
  pilT Acinetobacter baylyi ADP1

57.1

92.458

0.528
  pilT Legionella pneumophila strain ERS1305867

57.1

92.458

0.528
  pilT Pseudomonas aeruginosa PAK

55.856

93.017

0.52
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.326

96.089

0.503
  pilT Vibrio cholerae strain A1552

52.326

96.089

0.503
  pilT Neisseria gonorrhoeae MS11

54.154

90.782

0.492
  pilT Neisseria meningitidis 8013

54.154

90.782

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.729

95.81

0.486
  pilU Vibrio cholerae strain A1552

39.692

90.782

0.36

Multiple sequence alignment