Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CWI23_RS25190 Genome accession   NZ_CP025051
Coordinates   5231519..5231971 (-) Length   150 a.a.
NCBI ID   WP_023518088.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PB353     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5226519..5236971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI23_RS25170 (CWI23_25170) ampD 5227214..5227780 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CWI23_RS25175 (CWI23_25175) - 5227931..5230186 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  CWI23_RS25180 (CWI23_25180) nadC 5230472..5231320 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CWI23_RS25190 (CWI23_25190) pilA 5231519..5231971 (-) 453 WP_023518088.1 pilin Machinery gene
  CWI23_RS25195 (CWI23_25195) pilB 5232203..5233903 (+) 1701 WP_021264132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CWI23_RS25200 (CWI23_25200) pilC 5233907..5235127 (+) 1221 WP_031634696.1 type 4a pilus biogenesis protein PilC Machinery gene
  CWI23_RS25205 (CWI23_25205) pilD 5235131..5236003 (+) 873 WP_003112839.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CWI23_RS25210 (CWI23_25210) coaE 5236000..5236611 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  CWI23_RS25215 (CWI23_25215) yacG 5236608..5236808 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15686.02 Da        Isoelectric Point: 7.7814

>NTDB_id=257778 CWI23_RS25190 WP_023518088.1 5231519..5231971(-) (pilA) [Pseudomonas aeruginosa strain PB353]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVAIKDTGDGTVKFTFATGQSSPKNAGTEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=257778 CWI23_RS25190 WP_023518088.1 5231519..5231971(-) (pilA) [Pseudomonas aeruginosa strain PB353]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGCTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

65.789

100

0.667
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.651

99.333

0.473
  pilA Vibrio cholerae strain A1552

47.651

99.333

0.473
  pilA Vibrio cholerae C6706

47.651

99.333

0.473
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.333

100

0.453
  pilA Acinetobacter baumannii strain A118

44.595

98.667

0.44
  pilA Vibrio parahaemolyticus RIMD 2210633

43.243

98.667

0.427
  comP Acinetobacter baylyi ADP1

42

100

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.958

95.333

0.4
  pilA2 Legionella pneumophila strain ERS1305867

39.597

99.333

0.393
  pilA2 Legionella pneumophila str. Paris

38.926

99.333

0.387
  pilA/pilA1 Eikenella corrodens VA1

38.514

98.667

0.38
  pilA Ralstonia pseudosolanacearum GMI1000

38.889

96

0.373
  pilA Acinetobacter nosocomialis M2

41.353

88.667

0.367

Multiple sequence alignment