Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   COO91_RS21355 Genome accession   NZ_CP024785
Coordinates   4607111..4608238 (-) Length   375 a.a.
NCBI ID   WP_100900133.1    Uniprot ID   A0A2K8SUG6
Organism   Nostoc flagelliforme CCNUN1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4602111..4613238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COO91_RS21325 (COO91_04965) - 4602191..4602439 (-) 249 WP_100900127.1 DUF4926 domain-containing protein -
  COO91_RS21330 - 4602459..4602806 (-) 348 WP_100900128.1 DUF6883 domain-containing protein -
  COO91_RS49480 (COO91_04967) - 4603012..4603590 (-) 579 WP_157816566.1 RNA polymerase subunit sigma-24 -
  COO91_RS21340 (COO91_04968) - 4603868..4604890 (+) 1023 WP_100900130.1 saccharopine dehydrogenase-like oxidoreductase -
  COO91_RS21345 (COO91_04969) - 4605015..4605569 (-) 555 WP_404824134.1 hypothetical protein -
  COO91_RS21350 (COO91_04970) - 4605705..4606928 (-) 1224 WP_100900132.1 type II secretion system F family protein -
  COO91_RS21355 (COO91_04972) pilT 4607111..4608238 (-) 1128 WP_100900133.1 type IV pilus twitching motility protein PilT Machinery gene
  COO91_RS21360 (COO91_04973) pilB/pilB1 4608349..4610355 (-) 2007 WP_100900134.1 GspE/PulE family protein Machinery gene
  COO91_RS54880 (COO91_04975) - 4610884..4611018 (-) 135 WP_263982783.1 hypothetical protein -
  COO91_RS21365 (COO91_04976) grpE 4611254..4612003 (+) 750 WP_100900135.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41028.39 Da        Isoelectric Point: 7.4488

>NTDB_id=255420 COO91_RS21355 WP_100900133.1 4607111..4608238(-) (pilT) [Nostoc flagelliforme CCNUN1]
MEMMIEDLMEQLIEMGGSDMHLSAGLPPYFRISGKLTPIGEHVLTADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDIVREMCDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVGVFSQTLVSKKNPKPGEYGRVMAQEILIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADFYKAGTISFEAAMSKTSKPDEIQRLIGTSVPSQAAGAKPGAAAKAH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=255420 COO91_RS21355 WP_100900133.1 4607111..4608238(-) (pilT) [Nostoc flagelliforme CCNUN1]
ATGGAAATGATGATTGAAGACCTAATGGAGCAGTTGATTGAAATGGGTGGCTCCGATATGCATTTATCCGCAGGTTTGCC
TCCCTACTTTCGTATCAGTGGCAAACTCACCCCCATAGGTGAGCATGTATTAACAGCCGATCAATGTCAAAGGCTGATTT
TTAGTATGCTCAACAACACTCAGCGTAAAACCTTAGAGCAGACCTGGGAATTGGATTGTTCTTATGGTGTTAAGGGTTTG
GCTCGCTTCCGGGTGAATGTTTATAAAGAACGTGGTGCTTATGCCGCTTGCTTACGGGCATTAAGTTCTAAAATTCCTAA
CTTTGAAAAATTAGGTCTGCCAGATATAGTCCGGGAAATGTGCGATAAGCCCAGAGGACTTATTCTAGTGACAGGCCCTA
CAGGTTCCGGCAAGACAACTACCCTAGCGGCAATGATTGACTTGATCAACCGCACCAAGGCAGAGCATATTTTAACGGTG
GAAGATCCAATTGAATTTGTCTATGAACCAATTAAAAGTTTGGTTCACCAACGGCAACTAGGAGAAGATACTAAGAGCTT
TGCTAATGCTTTGAAAGCAGCCCTACGGGAAGACCCAGATATTATTCTGGTGGGGGAAATGCGGGATTTGGAAACGATTT
CTTTGGCGATTTCCGCAGCAGAAACAGGACACTTAGTATTTGGTACGCTACACACCAGTTCTGCCGCCCAAACTGTTGAC
CGGATTATCGACGTTTTCCCCCATGAAAGACAAACCCAAGTGCGGGTGCAATTGTCTAACTCGTTAGTGGGGGTATTTAG
CCAAACCTTGGTATCCAAGAAAAACCCTAAACCTGGTGAATATGGTCGGGTGATGGCTCAAGAAATTCTGATTGTCACTC
CTGCTATTTCCAACTTGATTCGAGAAGGCAAAACATCTCAAATTTACTCGGCTATTCAAACTGGCGGCAAATTGGGAATG
CAAACTCTGGAGAAGGTTTTAGCCGATTTTTACAAAGCAGGAACGATTTCCTTTGAAGCGGCGATGTCTAAAACTTCTAA
GCCAGATGAAATCCAACGTCTCATCGGTACTTCTGTACCGTCCCAGGCAGCAGGTGCTAAACCTGGTGCGGCTGCCAAAG
CGCATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K8SUG6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.441

93.067

0.507
  pilT Pseudomonas stutzeri DSM 10701

53.582

93.067

0.499
  pilT Acinetobacter baylyi ADP1

53.026

92.533

0.491
  pilT Legionella pneumophila strain Lp02

52.738

92.533

0.488
  pilT Legionella pneumophila strain ERS1305867

52.738

92.533

0.488
  pilT Acinetobacter baumannii strain A118

52.161

92.533

0.483
  pilT Acinetobacter nosocomialis M2

52.161

92.533

0.483
  pilT Acinetobacter baumannii D1279779

52.161

92.533

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.857

93.333

0.456
  pilT Neisseria gonorrhoeae MS11

50.442

90.4

0.456
  pilT Neisseria meningitidis 8013

50.147

90.4

0.453
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.2

0.451
  pilT Vibrio cholerae strain A1552

49.415

91.2

0.451
  pilU Acinetobacter baylyi ADP1

37.883

95.733

0.363

Multiple sequence alignment