Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FORC73_RS12070 Genome accession   NZ_CP024082
Coordinates   2585600..2586706 (-) Length   368 a.a.
NCBI ID   WP_000422567.1    Uniprot ID   A0A7Z7VNQ4
Organism   Vibrio cholerae strain FORC_073     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2580600..2591706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC73_RS12045 (FORC73_2280) gshB 2581212..2582168 (+) 957 WP_000593311.1 glutathione synthase -
  FORC73_RS12050 (FORC73_2281) - 2582204..2582767 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  FORC73_RS12055 (FORC73_2282) ruvX 2582848..2583270 (+) 423 WP_000091870.1 Holliday junction resolvase RuvX -
  FORC73_RS12060 (FORC73_2283) tyrS 2583295..2584575 (-) 1281 WP_001088167.1 tyrosine--tRNA ligase -
  FORC73_RS12065 (FORC73_2284) - 2584684..2585481 (-) 798 WP_001123227.1 helix-turn-helix transcriptional regulator -
  FORC73_RS12070 (FORC73_2285) pilU 2585600..2586706 (-) 1107 WP_000422567.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FORC73_RS12075 (FORC73_2286) pilT 2586721..2587758 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  FORC73_RS12080 (FORC73_2287) - 2587784..2588494 (+) 711 WP_001256650.1 YggS family pyridoxal phosphate-dependent enzyme -
  FORC73_RS12085 (FORC73_2288) proC 2588547..2589365 (+) 819 WP_000437950.1 pyrroline-5-carboxylate reductase -
  FORC73_RS12090 (FORC73_2289) - 2589419..2589976 (+) 558 WP_001087257.1 YggT family protein -
  FORC73_RS12095 (FORC73_2290) yggU 2589976..2590266 (+) 291 WP_000718489.1 DUF167 family protein YggU -
  FORC73_RS12100 (FORC73_2291) - 2590312..2590743 (+) 432 WP_001233672.1 DUF4426 domain-containing protein -
  FORC73_RS12105 (FORC73_2292) - 2590813..2591415 (+) 603 WP_053034666.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41319.32 Da        Isoelectric Point: 6.5076

>NTDB_id=251745 FORC73_RS12070 WP_000422567.1 2585600..2586706(-) (pilU) [Vibrio cholerae strain FORC_073]
MELNQYLDGMLIHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=251745 FORC73_RS12070 WP_000422567.1 2585600..2586706(-) (pilU) [Vibrio cholerae strain FORC_073]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGATTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAATTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTGAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACGGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCGATT
GAGTTTGTGCATGAACACAAGCGCTGTATTGTGACGCAGCGAGAAGTGGGGCTGGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTACGCCAAGCCCCCGATATGATTTTGATTGGTGAAATCCGCAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACGGGGCATTTGTGTATGGCCACGCTGCACGCCAATAACGCCAACCAAGCGTTAGAGCGCATTTTGCAT
TTAGTGCCGAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GTGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z7VNQ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

99.728

100

0.997
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375

Multiple sequence alignment