Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FORC71_RS13470 Genome accession   NZ_CP023485
Coordinates   2683099..2683548 (+) Length   149 a.a.
NCBI ID   WP_025557611.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2678099..2688548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC71_RS13455 (FORC71_2430) pdhR 2680132..2680899 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FORC71_RS13460 (FORC71_2431) ampD 2681305..2681856 (-) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC71_RS13465 (FORC71_2432) nadC 2681949..2682836 (+) 888 WP_005484834.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC71_RS13470 (FORC71_2433) pilA 2683099..2683548 (+) 450 WP_025557611.1 pilin Machinery gene
  FORC71_RS13475 (FORC71_2434) pilB 2683552..2685237 (+) 1686 WP_120416796.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC71_RS13480 (FORC71_2435) pilC 2685261..2686484 (+) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  FORC71_RS13485 (FORC71_2436) pilD 2686549..2687418 (+) 870 WP_025533026.1 A24 family peptidase Machinery gene
  FORC71_RS13490 (FORC71_2437) coaE 2687419..2688033 (+) 615 WP_065779040.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15582.76 Da        Isoelectric Point: 7.8194

>NTDB_id=247621 FORC71_RS13470 WP_025557611.1 2683099..2683548(+) (pilA) [Vibrio parahaemolyticus strain FORC_071]
MKHSKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVAKSELGAGLATITSVRTNVEDYIVTNGSFPDGTTAGQLTTD
LGVIQPNNGAITFDDANSNILLTFAGSGNSPDVNNAKIALNRAAGGTWTCKATLDAKLLPKSCSKDANL

Nucleotide


Download         Length: 450 bp        

>NTDB_id=247621 FORC71_RS13470 WP_025557611.1 2683099..2683548(+) (pilA) [Vibrio parahaemolyticus strain FORC_071]
ATGAAACACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTAGTAGCGGTAATTGGTGTTTT
GGCGGCGATTGCTATCCCTCAATACCAAAACTATGTGGCCAAGTCAGAGTTGGGGGCTGGACTAGCAACAATAACCTCAG
TTCGAACTAATGTTGAAGATTACATTGTTACAAATGGTAGTTTTCCTGATGGCACAACTGCTGGCCAGCTAACAACAGAC
TTAGGAGTGATTCAGCCAAACAATGGTGCAATAACTTTTGATGACGCCAATAGCAACATCCTGTTAACTTTTGCTGGCTC
TGGAAATAGCCCTGACGTAAATAATGCAAAAATAGCACTAAACAGAGCGGCTGGTGGTACATGGACATGTAAAGCCACCC
TAGACGCTAAACTACTTCCAAAATCATGTTCTAAGGATGCTAATTTGTAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

52.288

100

0.537
  pilA Vibrio cholerae strain A1552

52.288

100

0.537
  pilA Vibrio cholerae C6706

52.288

100

0.537
  pilA Pseudomonas aeruginosa PAK

45.638

100

0.456
  pilA Vibrio parahaemolyticus RIMD 2210633

47.794

91.275

0.436
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.667

96.644

0.403
  pilA Acinetobacter baumannii strain A118

40.58

92.617

0.376
  pilA2 Legionella pneumophila str. Paris

39.855

92.617

0.369
  pilA Haemophilus influenzae 86-028NP

36.242

100

0.362
  pilA Haemophilus influenzae Rd KW20

35.294

100

0.362
  pilA Acinetobacter nosocomialis M2

37.762

95.973

0.362
  pilA/pilA1 Eikenella corrodens VA1

33.333

100

0.362
  pilA2 Legionella pneumophila strain ERS1305867

39.13

92.617

0.362

Multiple sequence alignment