Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CHR26_RS09420 Genome accession   NZ_CP022560
Coordinates   1989956..1990951 (+) Length   331 a.a.
NCBI ID   WP_145913576.1    Uniprot ID   -
Organism   Pseudomonas putida strain B1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1984956..1995951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHR26_RS09390 (CHR26_09320) - 1985433..1985867 (-) 435 WP_016489642.1 DUF4426 domain-containing protein -
  CHR26_RS09395 (CHR26_09325) metW 1985890..1986510 (-) 621 WP_016489641.1 methionine biosynthesis protein MetW -
  CHR26_RS09400 (CHR26_09330) - 1986518..1987657 (-) 1140 WP_016489640.1 homoserine O-acetyltransferase -
  CHR26_RS09405 (CHR26_09335) - 1987746..1988336 (-) 591 WP_016489639.1 YggT family protein -
  CHR26_RS09410 (CHR26_09340) proC 1988347..1989165 (-) 819 WP_016489638.1 pyrroline-5-carboxylate reductase -
  CHR26_RS09415 (CHR26_09345) - 1989204..1989890 (-) 687 WP_145913575.1 YggS family pyridoxal phosphate-dependent enzyme -
  CHR26_RS09420 (CHR26_09350) pilT 1989956..1990951 (+) 996 WP_145913576.1 type IV pilus twitching motility protein PilT Machinery gene
  CHR26_RS09425 (CHR26_09355) - 1991005..1991631 (-) 627 WP_145913577.1 C40 family peptidase -
  CHR26_RS09430 (CHR26_09360) - 1991798..1992214 (+) 417 WP_016489634.1 NINE protein -
  CHR26_RS09435 (CHR26_09365) - 1992281..1992979 (-) 699 WP_016489633.1 SPOR domain-containing protein -
  CHR26_RS09440 (CHR26_09370) argS 1992981..1994717 (-) 1737 WP_145913578.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 35606.10 Da        Isoelectric Point: 6.6143

>NTDB_id=240777 CHR26_RS09420 WP_145913576.1 1989956..1990951(+) (pilT) [Pseudomonas putida strain B1]
MDVTDLLARAVDAGASDLHLAAGQIPMLRLEGDLQRLDMPALVPADLADGMAALLAEHQRRQWAEGDELDLALQLPALGR
FRLNLFRQLNGPAVTFRLIPGRIATVGELDLGDVYQAVAQCTDGLVLVGGPTGSGKSSTLATLLDQLNRDRALHIITLED
PVEVIHSSQRSLINQREIGRHSGGFAQGLRSALRQDPDVIMIGELRDLESVRLALRAAETGHLVLATVHTRSAASSIDRL
VEVFAAEEKPLVRAMLAESLRLVVVQTLVRRVDGGRVAAREVLVATPAVRNLVREGRMAQLCSVMQGGASQGMRTMEGAM
RGLKESGLIRG

Nucleotide


Download         Length: 996 bp        

>NTDB_id=240777 CHR26_RS09420 WP_145913576.1 1989956..1990951(+) (pilT) [Pseudomonas putida strain B1]
ATGGATGTGACCGACCTGTTGGCCCGTGCTGTGGATGCAGGGGCTTCGGACCTGCACCTGGCGGCGGGTCAGATCCCGAT
GCTGCGCCTTGAGGGCGACTTGCAGCGCCTGGACATGCCGGCGTTGGTGCCGGCAGACCTGGCCGACGGCATGGCGGCCT
TGCTGGCTGAACACCAACGGCGGCAATGGGCAGAGGGCGATGAGCTGGACCTGGCGCTGCAGCTGCCCGCCCTGGGCCGT
TTTCGGCTGAACCTGTTTCGCCAGTTGAACGGCCCTGCGGTTACCTTCCGGTTGATCCCGGGGCGGATCGCCACGGTCGG
TGAACTCGACCTAGGGGATGTGTATCAAGCTGTTGCGCAGTGCACTGATGGCCTGGTCCTGGTAGGTGGGCCTACGGGCA
GTGGCAAGTCCAGCACCCTGGCGACCTTGCTCGACCAACTGAACCGCGACCGAGCGCTGCATATCATCACCCTCGAAGAC
CCCGTCGAAGTTATCCACAGCAGCCAGCGCAGCCTGATCAATCAGCGTGAGATTGGCCGCCATAGCGGCGGGTTCGCCCA
GGGGCTGCGCAGTGCGCTGCGCCAGGACCCTGATGTGATCATGATCGGTGAGCTACGGGACCTGGAATCCGTTCGCCTGG
CCTTGCGCGCCGCCGAGACCGGGCACCTGGTGCTGGCGACCGTGCATACGCGCTCGGCGGCCAGCAGCATTGACCGATTG
GTGGAGGTATTTGCTGCCGAGGAAAAACCGTTGGTGCGGGCAATGCTGGCCGAATCGTTACGCCTCGTGGTGGTGCAGAC
GCTGGTCCGGCGTGTGGACGGTGGGCGGGTGGCGGCGCGGGAAGTGCTGGTGGCGACGCCGGCGGTGCGCAACCTGGTTC
GGGAGGGGCGAATGGCGCAGTTATGTTCGGTGATGCAGGGCGGGGCGTCGCAGGGGATGCGGACGATGGAAGGGGCGATG
CGGGGGCTTAAGGAGAGCGGACTGATCAGAGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.76

99.396

0.505
  pilT Acinetobacter baumannii D1279779

50.456

99.396

0.502
  pilT Acinetobacter nosocomialis M2

50.456

99.396

0.502
  pilT Acinetobacter baumannii strain A118

50.456

99.396

0.502
  pilT Pseudomonas stutzeri DSM 10701

50.152

99.396

0.498
  pilT Acinetobacter baylyi ADP1

49.848

99.396

0.495
  pilT Vibrio cholerae strain A1552

48.632

99.396

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.632

99.396

0.483
  pilT Neisseria meningitidis 8013

47.879

99.698

0.477
  pilT Neisseria gonorrhoeae MS11

47.576

99.698

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.97

99.698

0.468
  pilT Legionella pneumophila strain Lp02

46.505

99.396

0.462
  pilT Legionella pneumophila strain ERS1305867

46.505

99.396

0.462
  pilU Pseudomonas stutzeri DSM 10701

36.97

99.698

0.369

Multiple sequence alignment