Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BG910_RS00590 Genome accession   NZ_CP022278
Coordinates   117705..118838 (+) Length   377 a.a.
NCBI ID   WP_089035166.1    Uniprot ID   A0A220RZ54
Organism   Neisseria chenwenguii strain 10023     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 112705..123838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG910_RS00570 (BG910_00570) lepB 113176..114162 (-) 987 WP_089035162.1 signal peptidase I -
  BG910_RS00575 (BG910_00575) - 114244..114855 (-) 612 WP_089035163.1 GTP pyrophosphokinase -
  BG910_RS00580 (BG910_00580) lepA 114914..116707 (-) 1794 WP_089035164.1 translation elongation factor 4 -
  BG910_RS00585 (BG910_00585) - 116872..117573 (-) 702 WP_089035165.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  BG910_RS00590 (BG910_00590) pilU 117705..118838 (+) 1134 WP_089035166.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BG910_RS00595 (BG910_00595) - 118942..119412 (-) 471 WP_089035167.1 universal stress protein -
  BG910_RS00600 (BG910_00600) - 119579..120355 (+) 777 WP_089035168.1 ThiF family adenylyltransferase -
  BG910_RS00605 (BG910_00605) - 120370..121200 (+) 831 WP_089037064.1 ScpA family protein -
  BG910_RS00610 (BG910_00610) - 121454..122545 (+) 1092 WP_089035169.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42368.50 Da        Isoelectric Point: 6.2440

>NTDB_id=237830 BG910_RS00590 WP_089035166.1 117705..118838(+) (pilU) [Neisseria chenwenguii strain 10023]
MYDNTNPPGAKDELYTWLRHMNKYKGSDLFITAKFPPAMKVDGKITPITEEPLTAERCMEIAFSIMSTKQIEEFSTTNEC
NFAISLPDTSRFRVNAMVQRGATAMVFRSITSNIPKFETLNLPPVLKEVAMKKRGLVIFVGGTGSGKSTSLASMIDYRNE
NSNGHIITIEDPIEFVHEHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMATLH
ANSTNQALDRIINFFPEERRTQLLTDLSLNLQAFISQRLIPRDGGKGRVAAVEILLNSPLIAEMIHKGDVHNIKELMKKS
TSMGMQTFDQALYQLFENGDISFQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=237830 BG910_RS00590 WP_089035166.1 117705..118838(+) (pilU) [Neisseria chenwenguii strain 10023]
ATGTACGACAACACCAATCCCCCCGGCGCGAAAGACGAACTCTATACTTGGCTGCGCCACATGAACAAATACAAAGGTTC
GGATCTCTTCATCACCGCCAAATTCCCGCCCGCCATGAAAGTGGACGGCAAAATCACGCCGATTACCGAAGAGCCGCTCA
CCGCCGAGCGCTGCATGGAAATCGCATTTTCCATCATGAGCACCAAGCAGATTGAGGAATTTTCCACCACCAACGAATGC
AATTTCGCCATCAGCCTGCCCGACACCAGCCGCTTCCGCGTCAACGCAATGGTGCAGCGCGGTGCGACCGCTATGGTGTT
CCGCTCGATTACCAGCAACATCCCGAAATTCGAAACCCTCAACCTGCCACCCGTTTTGAAAGAAGTCGCCATGAAAAAAC
GCGGCCTCGTCATTTTCGTCGGCGGCACCGGTTCGGGCAAATCCACCTCGCTGGCCTCGATGATCGACTACCGCAACGAA
AACAGCAACGGCCACATCATCACCATCGAAGACCCGATCGAATTTGTTCACGAACACAAAAACTGCATCATCACCCAGCG
CGAAGTCGGCGTCGATACCGAAAACTGGTTTGCCGCGCTGAAAAACACCCTGCGCCAAGCGCCCGACGTGATTCTCATCG
GAGAAATCCGCGACCGTGAAACCATGGATTACGCGCTGGCGTTTGCCGAAACCGGCCACCTCTGCATGGCCACCCTACAC
GCCAACAGCACCAACCAGGCGCTCGACCGTATCATCAACTTCTTCCCCGAAGAGCGCCGCACCCAACTTCTGACCGACCT
CTCGCTCAACCTTCAGGCCTTCATTTCCCAACGCCTGATCCCGCGCGACGGCGGCAAAGGACGAGTCGCCGCCGTCGAAA
TCCTGCTCAATTCGCCGCTGATTGCCGAAATGATCCATAAAGGCGACGTCCACAACATCAAAGAGCTGATGAAAAAATCC
ACCTCAATGGGTATGCAGACCTTCGACCAGGCGCTGTACCAACTGTTTGAAAACGGCGACATCAGTTTCCAAGACGCCAT
AAAAAACGCCGACTCCGCCCACGACCTGCGCCTCGACATCCAACTGCGCAGCCGCCGCGCCCAAAACGCCGGCCCGGATT
TAGAGCTGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A220RZ54

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.08

93.369

0.57
  pilU Acinetobacter baylyi ADP1

59.184

90.981

0.538
  pilU Vibrio cholerae strain A1552

54.039

95.225

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.544

88.329

0.385
  pilT Pseudomonas aeruginosa PAK

43.598

87.003

0.379
  pilT Legionella pneumophila strain Lp02

43.827

85.942

0.377
  pilT Legionella pneumophila strain ERS1305867

43.827

85.942

0.377
  pilT Acinetobacter baumannii D1279779

43.21

85.942

0.371
  pilT Acinetobacter baumannii strain A118

43.21

85.942

0.371
  pilT Acinetobacter nosocomialis M2

43.21

85.942

0.371
  pilT Acinetobacter baylyi ADP1

42.683

87.003

0.371
  pilT Pseudomonas stutzeri DSM 10701

42.073

87.003

0.366
  pilT Vibrio cholerae strain A1552

42.105

85.676

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.105

85.676

0.361

Multiple sequence alignment