Detailed information NTG0023488

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: DP_RS07385
• Genome accession: NC_006138
• Coordinates: 1624720..1625148 (-)
• Length: 142 a.a.
• NCBI ID: WP_011188693.1
• Uniprot ID: Q6AN93
• Organism: Desulfotalea psychrophila LSv54
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1619720..1630148

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DP_RS07370 (DP1447)	-	1620495..1621799 (+)	1305	WP_041277754.1	pyridoxal phosphate-dependent aminotransferase	-
DP_RS07375	-	1622442..1622663 (-)	222	WP_041277755.1	hypothetical protein	-
DP_RS07380 (DP1451)	-	1622774..1624477 (-)	1704	WP_162096633.1	PglL family O-oligosaccharyltransferase	-
DP_RS07385 (DP1452)	pilA2	1624720..1625148 (-)	429	WP_011188693.1	pilin	Machinery gene
DP_RS17905 (DP1453)	-	1625298..1625507 (-)	210	WP_011188694.1	hypothetical protein	-
DP_RS07390 (DP1455)	-	1625947..1626402 (-)	456	WP_011188696.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
DP_RS18955 (DP1456)	-	1627055..1627441 (+)	387	WP_041277757.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
DP_RS16725 (DP1457)	-	1627445..1628005 (+)	561	WP_011188698.1	GspH/FimT family pseudopilin	-
DP_RS07405 (DP1458)	-	1628009..1628698 (+)	690	WP_011188699.1	PilW family protein	-
DP_RS16730 (DP1459)	-	1628707..1629174 (+)	468	WP_011188700.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
DP_RS07415 (DP1460)	-	1629171..1629662 (+)	492	WP_011188701.1	PilX N-terminal domain-containing pilus assembly protein	-

Sequence

Protein

• Length: 142 a.a.
• Molecular weight: 14885.25 Da
• Isoelectric Point: 7.7751

>NTDB_id=23488 DP_RS07385 WP_011188693.1 1624720..1625148(-) (pilA2) [Desulfotalea psychrophila LSv54]
MKKALKNQQGFTLIELMIVVAIIGILAAVAIPQYQNYTKRAKVTEGLNMAGAAKTAVSEYYQSQNVLPTSLAEAGISTVD
TDIVKSLGIGASGVITVTFKAPVLLDATIVLTPSLSTTGAGLTWTCTNTGTDMDNYVPANCR

Nucleotide

• Length: 429 bp

>NTDB_id=23488 DP_RS07385 WP_011188693.1 1624720..1625148(-) (pilA2) [Desulfotalea psychrophila LSv54]
ATGAAAAAGGCACTCAAGAACCAACAGGGCTTTACACTTATCGAGCTGATGATCGTTGTGGCAATCATCGGAATTTTGGC
AGCTGTAGCTATTCCTCAGTATCAGAACTACACTAAACGGGCCAAGGTAACAGAGGGCCTCAATATGGCCGGTGCTGCCA
AGACAGCGGTTAGCGAGTACTATCAATCTCAAAATGTTTTGCCAACAAGTCTGGCTGAGGCAGGTATCTCTACTGTCGAT
ACAGACATCGTTAAATCACTCGGAATAGGAGCCAGTGGAGTTATTACTGTTACCTTTAAGGCTCCAGTTCTACTTGATGC
CACTATTGTTCTCACTCCATCATTATCAACAACAGGAGCTGGCTTAACTTGGACATGTACAAACACAGGCACCGACATGG
ATAATTATGTCCCAGCAAACTGTCGTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (6-30)

• Pilin (bacterial filament) (Pilin) (40-142)

3D structure

• AlphaFold DB: Q6AN93

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	56.934	96.479	0.549
pilA2	Legionella pneumophila strain ERS1305867	56.204	96.479	0.542
pilA	Ralstonia pseudosolanacearum GMI1000	39.13	100	0.444
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	40.26	100	0.437
pilA	Vibrio cholerae strain A1552	40.26	100	0.437
pilA	Vibrio cholerae C6706	40.26	100	0.437
comP	Acinetobacter baylyi ADP1	41.333	100	0.437
pilA	Pseudomonas aeruginosa PAK	38.217	100	0.423
pilA	Haemophilus influenzae Rd KW20	42.336	96.479	0.408
pilE	Neisseria gonorrhoeae strain FA1090	36.306	100	0.401
pilA	Acinetobacter baumannii strain A118	38.889	100	0.394
pilA	Haemophilus influenzae 86-028NP	39.855	97.183	0.387
pilA	Vibrio parahaemolyticus RIMD 2210633	43.307	89.437	0.387
pilA/pilAII	Pseudomonas stutzeri DSM 10701	33.758	100	0.373