Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PLU_RS05835 Genome accession   NC_005126
Coordinates   1364634..1365671 (+) Length   345 a.a.
NCBI ID   WP_011145508.1    Uniprot ID   A0A6L9JTN1
Organism   Photorhabdus laumondii subsp. laumondii TTO1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1359634..1370671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PLU_RS05810 (plu1176) hemW 1360760..1361890 (-) 1131 WP_011145503.1 radical SAM family heme chaperone HemW -
  PLU_RS05815 (plu1177) - 1361883..1362476 (-) 594 WP_011145504.1 XTP/dITP diphosphatase -
  PLU_RS05820 (plu1178) - 1362503..1363051 (-) 549 WP_011145505.1 YggT family protein -
  PLU_RS05825 (plu1179) proC 1363070..1363891 (-) 822 WP_011145506.1 pyrroline-5-carboxylate reductase -
  PLU_RS05830 (plu1180) - 1363917..1364612 (-) 696 WP_011145507.1 YggS family pyridoxal phosphate-dependent enzyme -
  PLU_RS05835 (plu1181) pilT 1364634..1365671 (+) 1038 WP_011145508.1 type IV pilus twitching motility protein PilT Machinery gene
  PLU_RS05840 (plu1182) ruvX 1365726..1366145 (-) 420 WP_011145509.1 Holliday junction resolvase RuvX -
  PLU_RS05845 (plu1183) - 1366146..1366709 (-) 564 WP_011145510.1 YqgE/AlgH family protein -
  PLU_RS05850 (plu1184) gshB 1366819..1367769 (-) 951 WP_011145511.1 glutathione synthase -
  PLU_RS05855 (plu1185) rsmE 1367779..1368510 (-) 732 WP_011145512.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PLU_RS05860 (plu1186) - 1368773..1369447 (+) 675 WP_082302879.1 prepilin peptidase -
  PLU_RS05865 (plu1187) - 1369496..1370137 (-) 642 WP_011145514.1 DsbA family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38069.63 Da        Isoelectric Point: 7.3094

>NTDB_id=23109 PLU_RS05835 WP_011145508.1 1364634..1365671(+) (pilT) [Photorhabdus laumondii subsp. laumondii TTO1]
MNMDKLVALSVKHNASDLHLCIGQVPVLRINGVLCPQTQLQSVNNLLLEEWSRQFLSENQRQQLLTSGHVDFALETENGQ
RLRGNIFRQHSGLSAVLRLIHSQCSTLEQLKAPEIIREMALREESGLILVTGATGSGKSTTLTAIVDMLNSHCCQHIITL
EDPVEFIHHSRNCLIQQRQIGLDSPSYQAALKGALRQDPDVLLLGELRDKETIRLALTAAETGHLVLSTLHTRGAVQAID
RLVDIFSAEEKGWVRSQLAGSLKAVIAQQLVSAQGGGRVAIYEVLVVNQAVSHLIREGKNHQITTLMQTGAASGMQTYEQ
GWQQRKLQGLLAEQTERKIKENAIS

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=23109 PLU_RS05835 WP_011145508.1 1364634..1365671(+) (pilT) [Photorhabdus laumondii subsp. laumondii TTO1]
ATGAATATGGATAAGCTAGTGGCCCTTAGTGTAAAGCATAATGCTTCAGATCTGCATCTTTGTATTGGGCAAGTTCCTGT
ATTACGTATAAATGGAGTGCTTTGTCCGCAAACTCAACTGCAATCTGTAAATAATCTATTGCTGGAAGAGTGGAGTAGGC
AGTTTTTATCAGAAAATCAGCGACAACAGCTATTAACTAGTGGACATGTGGATTTTGCGTTGGAAACAGAAAATGGCCAA
CGATTGAGAGGCAACATCTTTCGTCAGCACTCCGGGTTGTCAGCCGTTTTGCGTTTAATTCACTCTCAGTGTTCAACTCT
GGAACAGCTAAAAGCACCTGAAATTATCCGTGAAATGGCTTTGCGGGAAGAGAGTGGGTTGATCTTGGTAACCGGCGCTA
CAGGCAGCGGAAAGTCGACAACGTTAACTGCCATTGTGGATATGCTGAACAGCCACTGTTGTCAGCACATTATTACATTG
GAAGATCCTGTGGAATTTATCCACCATAGTCGCAATTGTTTGATACAACAACGGCAAATTGGGTTGGATTCTCCCAGTTA
TCAGGCTGCGTTAAAAGGGGCTCTCCGGCAAGATCCTGATGTATTGTTGTTGGGTGAGCTGCGGGATAAAGAGACAATAC
GCTTGGCGTTAACCGCGGCAGAGACTGGGCATTTGGTATTGTCGACTTTGCATACCCGAGGTGCTGTACAGGCAATTGAC
CGGCTGGTGGATATTTTTTCCGCTGAAGAAAAAGGGTGGGTACGTAGCCAGCTAGCAGGCAGCCTGAAAGCTGTGATAGC
ACAGCAATTAGTCTCTGCCCAGGGAGGGGGACGAGTTGCTATATATGAAGTATTGGTAGTTAATCAGGCAGTGAGTCATC
TGATACGAGAAGGAAAAAATCATCAAATAACAACGCTTATGCAGACGGGCGCTGCCAGTGGGATGCAAACCTATGAACAA
GGCTGGCAGCAGCGTAAATTGCAGGGATTACTGGCAGAGCAAACGGAAAGGAAGATTAAAGAAAATGCTATCAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6L9JTN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.986

100

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.986

100

0.49
  pilT Neisseria gonorrhoeae MS11

46.286

100

0.47
  pilT Neisseria meningitidis 8013

46.286

100

0.47
  pilT Acinetobacter baylyi ADP1

45.272

100

0.458
  pilT Pseudomonas aeruginosa PAK

44.126

100

0.446
  pilT Pseudomonas stutzeri DSM 10701

45.697

97.681

0.446
  pilT Legionella pneumophila strain ERS1305867

45.133

98.261

0.443
  pilT Legionella pneumophila strain Lp02

45.133

98.261

0.443
  pilT Acinetobacter baumannii D1279779

44.575

98.841

0.441
  pilT Acinetobacter baumannii strain A118

44.575

98.841

0.441
  pilT Acinetobacter nosocomialis M2

44.575

98.841

0.441
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.515

96.812

0.412
  pilU Pseudomonas stutzeri DSM 10701

39.091

95.652

0.374

Multiple sequence alignment