Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DFI_RS02905 Genome accession   NZ_CP021081
Coordinates   580033..581307 (+) Length   424 a.a.
NCBI ID   WP_022800180.1    Uniprot ID   A0A221STY4
Organism   Deinococcus ficus strain CC-FR2-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 575033..586307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DFI_RS02890 (DFI_02890) pgeF 575860..576621 (+) 762 WP_027463381.1 peptidoglycan editing factor PgeF -
  DFI_RS02895 (DFI_02895) - 576618..577130 (+) 513 WP_027463382.1 YqeG family HAD IIIA-type phosphatase -
  DFI_RS02900 (DFI_02900) pilB 577239..579905 (+) 2667 WP_022800181.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  DFI_RS02905 (DFI_02905) pilT 580033..581307 (+) 1275 WP_022800180.1 type IV pilus twitching motility protein PilT Machinery gene
  DFI_RS02910 (DFI_02910) lon 581371..583806 (-) 2436 WP_022800179.1 endopeptidase La -
  DFI_RS02915 (DFI_02915) clpX 583957..585162 (-) 1206 WP_027463383.1 ATP-dependent Clp protease ATP-binding subunit ClpX -
  DFI_RS02920 (DFI_02920) clpP 585159..585770 (-) 612 WP_108912914.1 ATP-dependent Clp protease proteolytic subunit -
  DFI_RS02925 (DFI_02925) - 585900..586082 (-) 183 WP_027463384.1 hypothetical protein -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 45804.33 Da        Isoelectric Point: 7.5532

>NTDB_id=228158 DFI_RS02905 WP_022800180.1 580033..581307(+) (pilT) [Deinococcus ficus strain CC-FR2-10]
MTQPTADITDILKFAAEKGASDVILTAGLAPQFKLHGTYDSQGFPEIGPTDTRKLMYSMMNEKQQRTFEEKRELDFSFAL
GERARFRVNAFMQRGHVGGVMRLIPTQVKTVGEMGLPSQVVDLANAPRGLVLVTGPTGSGKSTTLAAMIDHINITKKLHI
VTIEDPIEFMHQHKGCIVNQREIGSDTLGFNEALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPE
SIDRIVDVFPEEQQAQIRVQLANNLVGIMTQQLLPRLDGAGRILAYELLIANPAVRALIREGKTYQITSVMQTGAREGMI
TMDAYLANLYRRRLISFDIGADRAVDAKEFARLANDPNAPMPTLGSASNAAQAGLSVNTPSGARGSTPASAYATDPGLTV
RPVNETGRNSATPAPAGGNPFGRR

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=228158 DFI_RS02905 WP_022800180.1 580033..581307(+) (pilT) [Deinococcus ficus strain CC-FR2-10]
ATGACCCAGCCCACCGCCGACATCACCGACATCCTGAAGTTCGCCGCCGAGAAGGGCGCCTCCGACGTCATCCTGACCGC
CGGTCTGGCCCCGCAGTTCAAACTGCACGGCACCTACGACTCGCAGGGCTTCCCGGAAATCGGCCCGACCGACACCCGGA
AACTCATGTACAGCATGATGAACGAAAAGCAGCAGCGCACCTTCGAGGAAAAACGCGAACTGGACTTCAGTTTCGCCCTC
GGGGAACGCGCCCGCTTCCGCGTGAACGCCTTCATGCAGCGCGGCCACGTGGGCGGCGTGATGCGCCTGATTCCCACCCA
GGTCAAGACGGTGGGGGAGATGGGCCTGCCCAGCCAGGTGGTGGACCTCGCGAACGCCCCGCGCGGGCTGGTGCTCGTGA
CCGGGCCCACCGGGTCGGGGAAAAGCACCACGCTGGCCGCGATGATCGACCACATCAACATCACGAAGAAGCTGCACATC
GTGACCATCGAGGACCCGATCGAGTTCATGCACCAGCACAAGGGCTGCATCGTGAACCAGCGCGAGATCGGCTCCGACAC
CCTGGGCTTCAACGAGGCGCTGCGCGCCGTGCTGCGTCAGGCGCCGGACGTGATCCTGGTGGGCGAAATGCGTGACTACG
AGACCATCAAGGCCGCCGTGACCGCCGCCGAAACCGGGCACCTGGTGATGGGCACCCTGCACACCAACAGCGCGCCCGAA
TCCATCGACCGTATCGTGGACGTCTTCCCGGAAGAGCAGCAGGCGCAGATTCGCGTGCAGCTCGCGAACAACCTGGTGGG
CATCATGACCCAGCAGCTGCTGCCCCGCCTGGACGGCGCCGGACGCATCCTGGCGTACGAGCTGCTGATCGCCAACCCGG
CCGTGCGCGCCCTGATCCGCGAGGGCAAGACGTACCAGATCACCAGCGTCATGCAGACCGGCGCGCGCGAGGGCATGATC
ACCATGGACGCGTACCTCGCGAACCTGTACCGCCGCCGCCTGATCAGCTTCGACATCGGCGCGGACCGCGCCGTGGACGC
CAAGGAGTTCGCGCGCCTCGCGAACGACCCGAACGCGCCCATGCCCACCCTGGGCAGCGCCTCGAACGCCGCGCAGGCGG
GCCTGAGCGTGAACACCCCCAGCGGAGCGCGCGGGTCCACCCCCGCCAGCGCCTACGCCACCGACCCGGGCCTGACCGTG
CGGCCGGTGAACGAGACGGGCCGCAACTCGGCCACGCCCGCGCCCGCCGGCGGGAATCCCTTCGGCCGCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A221STY4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

78.249

88.915

0.696
  pilT Pseudomonas aeruginosa PAK

52.663

79.717

0.42
  pilT Pseudomonas stutzeri DSM 10701

51.744

81.132

0.42
  pilT Neisseria meningitidis 8013

51.014

81.368

0.415
  pilT Acinetobacter baylyi ADP1

52.87

78.066

0.413
  pilT Acinetobacter baumannii D1279779

53.03

77.83

0.413
  pilT Acinetobacter baumannii strain A118

53.03

77.83

0.413
  pilT Acinetobacter nosocomialis M2

53.03

77.83

0.413
  pilT Neisseria gonorrhoeae MS11

50.291

81.132

0.408
  pilT Legionella pneumophila strain Lp02

47.674

81.132

0.387
  pilT Legionella pneumophila strain ERS1305867

47.674

81.132

0.387
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.246

81.368

0.384
  pilT Vibrio cholerae strain A1552

47.246

81.368

0.384

Multiple sequence alignment