Detailed information NTG0228157

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DFI_RS02900
• Genome accession: NZ_CP021081
• Coordinates: 577239..579905 (+)
• Length: 888 a.a.
• NCBI ID: WP_022800181.1
• Uniprot ID: A0A221STZ8
• Organism: Deinococcus ficus strain CC-FR2-10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 572239..584905

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DFI_RS02870 (DFI_02870)	serS	573013..574290 (-)	1278	WP_027463378.1	serine--tRNA ligase	-
DFI_RS02875 (DFI_02875)	gatC	574290..574580 (-)	291	WP_022800186.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
DFI_RS02880 (DFI_02880)	-	574619..574930 (-)	312	WP_027463379.1	DUF6896 domain-containing protein	-
DFI_RS02885 (DFI_02885)	-	574935..575738 (-)	804	WP_027463380.1	enoyl-ACP reductase FabI	-
DFI_RS02890 (DFI_02890)	pgeF	575860..576621 (+)	762	WP_027463381.1	peptidoglycan editing factor PgeF	-
DFI_RS02895 (DFI_02895)	-	576618..577130 (+)	513	WP_027463382.1	YqeG family HAD IIIA-type phosphatase	-
DFI_RS02900 (DFI_02900)	pilB	577239..579905 (+)	2667	WP_022800181.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
DFI_RS02905 (DFI_02905)	pilT	580033..581307 (+)	1275	WP_022800180.1	type IV pilus twitching motility protein PilT	Machinery gene
DFI_RS02910 (DFI_02910)	lon	581371..583806 (-)	2436	WP_022800179.1	endopeptidase La	-

Sequence

Protein

• Length: 888 a.a.
• Molecular weight: 97000.76 Da
• Isoelectric Point: 4.8915

>NTDB_id=228157 DFI_RS02900 WP_022800181.1 577239..579905(+) (pilB) [Deinococcus ficus strain CC-FR2-10]
MALSIGDRRLGAILLEQGYINDTDLQKALSRHAEVGGRLADVLIDTGLVAEKRIARAVEEALGIPLVNLTIVNPDVEALK
RVRAETASTHHAFPFAVEAGKLRVALVDPLSTVSIEALEDDSNMDIEVYQALRSQIQWAIATHYPELNLPVPEADEALGG
SPVEERMGDRLVSRGLITKEQLQAALDVQAQTGDLLGVTLVSQKAITEEQLYESLAEQYGYPFVHNPRDFNPSQEVLGCM
LRADALRLNAVPVDDSVLSVTVVTSDPTKKDDIQAVVGRPVSLVLTRPRDLENMIERLYPQRGRLGEAMVNEGVLSREQL
REALQVQAREGKVKPLGEVIMELGFAEEGEISSALEKQNSGGGRLEDTLVQSGKLSPEMLARSLAAQLGYDFLDPIQNPP
DAGVIKEIPEQTARRYSVIPVRYQGNAIVIAMKDPRNVFALDDLKLLTGREVIPAVMAEKDITRLIERYYGSNDMAALNE
QLSQGGKKEAAEEVDLSAGLDDNAVVRVVDSLIREAALQDVSDIHIEPSETAVRVRYRIDGVLREQPPLPKASAASVLAR
IKILGHLDISERRVPQDGRVRFKKGSIDIDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSDYNMQRYSDVIQKPNG
IFLVTGPTGSGKSFTSFSTLKRIAVPEKNTTTIEDPVEYEIPGIIQSQVNNTAGLTFARALRAFLRQDPDIIFVGEIRDQ
ETAKIAVEAALTGHLVLATLHTNDAPGAIIRLDEMGVEPFNISASVVGVLAQRLVRRVCQECKQPTNADPDVLRRLGITE
KDIQGAHLMRGAGCPRCGGTGYKGRMGIHELMVIDEPLRHAIGSGKTASEIKEVALTQSGMKTLRQDGIEKALEGRTTLE
EVLAVTSN

Nucleotide

• Length: 2667 bp

>NTDB_id=228157 DFI_RS02900 WP_022800181.1 577239..579905(+) (pilB) [Deinococcus ficus strain CC-FR2-10]
ATGGCTCTTTCTATCGGTGACCGGCGCCTGGGCGCCATCCTGCTCGAACAGGGGTACATCAACGACACGGACCTGCAAAA
AGCCCTGTCCCGGCACGCCGAGGTGGGGGGCCGCCTCGCGGACGTCCTGATCGACACCGGCCTCGTCGCCGAGAAACGCA
TCGCGCGGGCGGTGGAGGAAGCGCTGGGGATTCCGCTGGTGAACCTCACCATCGTGAACCCGGACGTGGAGGCCCTCAAG
CGCGTGCGCGCCGAGACGGCCAGCACCCACCACGCCTTCCCGTTCGCGGTGGAGGCCGGGAAGCTGCGCGTGGCCCTGGT
GGATCCGCTCTCCACCGTGAGCATCGAGGCGCTGGAAGACGACAGCAACATGGACATCGAGGTGTACCAGGCGCTGCGCA
GCCAGATCCAGTGGGCGATCGCCACGCACTACCCCGAACTGAACCTGCCGGTCCCCGAGGCGGACGAGGCGCTGGGCGGC
TCCCCGGTGGAGGAACGCATGGGGGACCGGCTGGTCTCCCGCGGCCTGATCACGAAAGAGCAGTTGCAGGCGGCGCTGGA
CGTGCAGGCGCAGACCGGGGACCTGCTGGGCGTCACGCTGGTGTCGCAGAAGGCCATCACCGAGGAGCAGCTGTACGAGT
CCCTGGCCGAGCAGTACGGGTACCCGTTCGTGCACAACCCCCGGGACTTCAACCCGTCGCAGGAGGTGCTGGGCTGCATG
CTCCGCGCCGACGCGCTGCGCCTGAACGCCGTGCCGGTGGACGACTCGGTGCTGTCCGTGACGGTCGTGACCAGCGACCC
CACCAAGAAGGACGACATTCAGGCCGTGGTGGGCCGCCCGGTGAGCCTGGTGCTGACCCGCCCGCGCGACCTGGAGAACA
TGATCGAGCGCCTGTACCCGCAGCGCGGCCGGCTGGGCGAGGCGATGGTGAACGAGGGTGTGCTCTCCCGCGAGCAGCTG
CGCGAGGCGCTGCAGGTGCAGGCCCGCGAGGGCAAGGTCAAGCCGCTGGGCGAGGTGATCATGGAACTCGGCTTCGCCGA
GGAAGGCGAGATCAGCTCCGCGCTGGAAAAACAGAACTCCGGCGGGGGCCGCCTGGAGGACACGCTGGTGCAGTCCGGGA
AGCTCAGCCCGGAGATGCTGGCCCGCTCGCTGGCCGCGCAGCTCGGCTACGATTTCCTGGACCCCATCCAGAACCCGCCG
GACGCCGGGGTGATCAAGGAGATTCCCGAGCAGACCGCGCGGCGCTACTCGGTGATCCCGGTGCGCTACCAGGGCAACGC
GATCGTGATCGCCATGAAGGACCCGCGCAACGTGTTCGCGCTGGACGACCTGAAACTCCTGACCGGGCGCGAGGTGATCC
CGGCCGTGATGGCCGAGAAGGACATCACCCGCCTGATCGAGCGGTACTACGGCAGCAACGACATGGCCGCCCTGAACGAG
CAGCTCAGCCAGGGCGGGAAGAAGGAAGCGGCCGAGGAGGTGGACCTCTCCGCCGGCCTGGACGACAATGCGGTCGTGCG
CGTGGTGGACAGCCTGATCCGCGAGGCGGCGCTGCAGGACGTCAGCGACATTCACATCGAGCCGAGCGAGACGGCCGTGC
GCGTCCGCTACCGCATCGACGGCGTGCTGCGCGAGCAGCCGCCCCTCCCGAAGGCCAGTGCGGCCAGCGTGCTGGCCCGC
ATCAAGATCCTGGGGCACCTGGACATCAGTGAGCGCCGCGTCCCGCAGGACGGCCGCGTGCGCTTCAAGAAAGGCAGCAT
CGACATCGACCTGCGCCTGTCCACGCTGCCCACGGTGTACGGCGAGAAGGCCGTGATGCGCCTGCTGCAGAAGGCCAGCA
ACATTCCCGAGGTGGAACAGCTGGGATTCAGCGATTACAACATGCAGCGCTACAGCGACGTGATCCAGAAACCCAACGGG
ATCTTCCTGGTGACCGGCCCCACCGGGTCCGGGAAGTCCTTCACGTCCTTCTCGACCCTCAAGCGCATCGCGGTGCCGGA
GAAGAACACCACCACCATCGAGGACCCCGTCGAGTACGAGATTCCCGGGATCATCCAGTCGCAGGTGAACAACACCGCCG
GCCTCACCTTCGCCCGCGCGCTGCGCGCCTTCCTGCGTCAGGACCCGGACATCATCTTCGTGGGGGAAATCCGCGACCAG
GAAACCGCGAAGATCGCCGTGGAAGCGGCCCTGACCGGCCACCTGGTGCTCGCCACGCTGCACACCAACGACGCGCCCGG
CGCGATCATCCGCCTGGACGAGATGGGCGTGGAGCCCTTCAACATCAGCGCGTCCGTGGTGGGGGTGCTCGCTCAGCGCC
TGGTGCGCCGCGTGTGCCAGGAGTGCAAACAGCCCACCAACGCCGACCCGGACGTCCTGCGCCGCCTGGGCATCACGGAA
AAGGACATCCAGGGCGCGCACCTGATGCGCGGCGCCGGCTGCCCCCGCTGCGGCGGCACCGGGTACAAGGGCCGCATGGG
CATCCACGAACTGATGGTGATCGACGAGCCCCTGCGCCACGCCATCGGGTCTGGGAAGACCGCCAGCGAGATCAAGGAGG
TCGCCCTCACGCAGAGCGGCATGAAGACCCTCCGGCAGGACGGCATCGAGAAGGCCCTGGAGGGCCGCACCACCCTGGAA
GAGGTCCTGGCCGTCACCAGCAACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (512-777)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (200-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-473)

3D structure

• AlphaFold DB: A0A221STZ8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	73.649	100	0.736
pilF	Thermus thermophilus HB27	56.775	100	0.571