Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BMF81_RS11450 Genome accession   NZ_CP020114
Coordinates   2584247..2585371 (+) Length   374 a.a.
NCBI ID   WP_006197907.1    Uniprot ID   -
Organism   Nodularia spumigena UHCC 0039     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2579247..2590371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMF81_RS11435 (BMF81_02245) grpE 2580651..2581397 (-) 747 WP_044482724.1 nucleotide exchange factor GrpE -
  BMF81_RS24955 - 2581821..2581928 (-) 108 Protein_2200 RNA-guided endonuclease TnpB family protein -
  BMF81_RS24695 - 2581932..2582069 (+) 138 WP_173403273.1 DUF6364 family protein -
  BMF81_RS11445 pilB/pilB1 2582169..2584184 (+) 2016 WP_006197908.1 GspE/PulE family protein Machinery gene
  BMF81_RS11450 pilT 2584247..2585371 (+) 1125 WP_006197907.1 type IV pilus twitching motility protein PilT Machinery gene
  BMF81_RS11455 - 2585406..2586623 (+) 1218 WP_006197906.1 type II secretion system F family protein -
  BMF81_RS11460 - 2586750..2587301 (+) 552 WP_006197905.1 hypothetical protein -
  BMF81_RS11465 (BMF81_02251) - 2587413..2588417 (-) 1005 WP_006197904.1 hypothetical protein -
  BMF81_RS11470 - 2588640..2589059 (+) 420 WP_006197903.1 XisH family protein -
  BMF81_RS11475 - 2589047..2589385 (+) 339 WP_006197902.1 XisI protein -
  BMF81_RS11485 - 2589681..2590181 (+) 501 WP_006197901.1 hypothetical protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40919.22 Da        Isoelectric Point: 7.8316

>NTDB_id=221307 BMF81_RS11450 WP_006197907.1 2584247..2585371(+) (pilT) [Nodularia spumigena UHCC 0039]
MEMMIEDLMEQMIEMGGSDMHLSAGLPPYFRISGKLTPIGDQVLSADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDIVREMSDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLNEDTKSFSNALRAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHEKQTQVRVQLSNSLVAIFSQTLVSKKNPKPGEFGRVMAQEILVVTPAISNLIREGKTSQIYSTIQTGGKLGM
QTLEKVLADLYKAGTISFEAAMSKTSKPDEVQRLIGTSAPPAGARPGAGAAKGR

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=221307 BMF81_RS11450 WP_006197907.1 2584247..2585371(+) (pilT) [Nodularia spumigena UHCC 0039]
ATGGAAATGATGATTGAAGACTTGATGGAACAGATGATTGAAATGGGTGGCTCGGATATGCATTTATCCGCAGGTTTACC
TCCCTACTTTCGTATTAGTGGCAAATTGACTCCCATCGGAGACCAAGTATTGAGTGCAGACCAATGCCAAAGGCTAATTT
TTAGTATGCTCAATAATACCCAGCGTAAAACTTTAGAACAAACTTGGGAGTTGGATTGTTCCTATGGTGTCAAGGGTTTA
GCTCGTTTTCGGGTAAATGTCTATAAAGAACGTGGTGCTTATGCGGCTTGTTTACGGGCGCTGAGTTCCAAAATTCCTAA
CTTTGAAAAATTAGGTCTGCCAGATATAGTGCGGGAAATGTCCGATAAGCCCAGAGGATTAATTCTGGTGACAGGTCCCA
CAGGTTCGGGAAAAACAACCACCCTAGCGGCAATGATTGACTTAATTAACCGCACCAAAGCAGAACATATTCTCACGGTG
GAAGATCCGATTGAATTTGTTTATGAACCGATTAAAAGCCTAGTCCACCAACGACAACTCAATGAAGATACCAAGAGTTT
TTCTAATGCTTTGAGAGCTGCATTACGGGAAGATCCAGATATTATTCTGGTGGGGGAAATGCGGGATTTAGAAACGATTT
CTTTGGCTATTTCGGCGGCAGAAACAGGACACTTGGTATTTGGGACTTTGCACACGAGTTCGGCGGCACAGACTGTTGAC
CGGATTATTGACGTGTTTCCCCATGAGAAACAAACTCAAGTGCGGGTGCAGTTATCTAACTCTTTAGTAGCCATATTTAG
CCAAACTTTAGTCAGCAAGAAAAACCCTAAACCTGGTGAATTTGGACGGGTGATGGCTCAAGAAATTCTTGTTGTTACGC
CTGCTATTTCTAACTTAATTCGGGAAGGTAAAACATCGCAAATTTACTCGACTATCCAGACGGGCGGTAAATTGGGGATG
CAAACTTTAGAAAAGGTTTTAGCTGATTTGTACAAAGCTGGAACTATTTCTTTTGAAGCCGCAATGTCTAAAACTTCCAA
GCCTGATGAAGTTCAACGTCTCATCGGTACTTCTGCACCACCAGCAGGAGCAAGACCTGGTGCTGGCGCTGCGAAAGGGC
GTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.043

92.781

0.511
  pilT Pseudomonas stutzeri DSM 10701

54.467

92.781

0.505
  pilT Legionella pneumophila strain Lp02

53.314

92.781

0.495
  pilT Legionella pneumophila strain ERS1305867

53.314

92.781

0.495
  pilT Acinetobacter baylyi ADP1

53.026

92.781

0.492
  pilT Acinetobacter baumannii strain A118

51.873

92.781

0.481
  pilT Acinetobacter baumannii D1279779

51.873

92.781

0.481
  pilT Acinetobacter nosocomialis M2

51.873

92.781

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.429

93.583

0.463
  pilT Neisseria gonorrhoeae MS11

50.442

90.642

0.457
  pilT Neisseria meningitidis 8013

50.442

90.642

0.457
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.708

91.444

0.455
  pilT Vibrio cholerae strain A1552

49.708

91.444

0.455

Multiple sequence alignment