Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   BMF81_RS11445 Genome accession   NZ_CP020114
Coordinates   2582169..2584184 (+) Length   671 a.a.
NCBI ID   WP_006197908.1    Uniprot ID   -
Organism   Nodularia spumigena UHCC 0039     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2577169..2589184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMF81_RS11430 (BMF81_02244) dnaK 2578393..2580357 (-) 1965 WP_006198631.1 molecular chaperone DnaK -
  BMF81_RS11435 (BMF81_02245) grpE 2580651..2581397 (-) 747 WP_044482724.1 nucleotide exchange factor GrpE -
  BMF81_RS24955 - 2581821..2581928 (-) 108 Protein_2200 RNA-guided endonuclease TnpB family protein -
  BMF81_RS24695 - 2581932..2582069 (+) 138 WP_173403273.1 DUF6364 family protein -
  BMF81_RS11445 pilB/pilB1 2582169..2584184 (+) 2016 WP_006197908.1 GspE/PulE family protein Machinery gene
  BMF81_RS11450 pilT 2584247..2585371 (+) 1125 WP_006197907.1 type IV pilus twitching motility protein PilT Machinery gene
  BMF81_RS11455 - 2585406..2586623 (+) 1218 WP_006197906.1 type II secretion system F family protein -
  BMF81_RS11460 - 2586750..2587301 (+) 552 WP_006197905.1 hypothetical protein -
  BMF81_RS11465 (BMF81_02251) - 2587413..2588417 (-) 1005 WP_006197904.1 hypothetical protein -
  BMF81_RS11470 - 2588640..2589059 (+) 420 WP_006197903.1 XisH family protein -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 74895.85 Da        Isoelectric Point: 5.0488

>NTDB_id=221306 BMF81_RS11445 WP_006197908.1 2582169..2584184(+) (pilB/pilB1) [Nodularia spumigena UHCC 0039]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGYVNSEQMRLALIESRKSGKPLTEVLESITGKQLAPEFLRQYKKQQLFELK
ILYGVEFLDPEINQIGNPKACNLIHTLIPADICRRHRLVPLSRHDDQTPPSVLVAMVDPDNLEASDDLNRILRPQGLALK
CMVITQEDYQQLINLYLDDLAVRQKQLEKEKFTDINQDLENLDDIDIDDSSEEMEVDLGVVMKGAEDAPVINLVNRVLAK
ALHEGVSDIHIEPQEENLRIRFRKDGVLSEAFDPLPKKIVMAVTARFKIISNLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLQIVQDMVSRPFGLILVTGPTGSGKTTSLYSALAEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFSTALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRIPYTPTTEELARYGLSASQSASVTFYKANTLTLGEITEAKAINQEVCPKCN
GSGYKGRCGVYEVMRITENLQTLINQEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAK
RKSGLTCVTCSAILQPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=221306 BMF81_RS11445 WP_006197908.1 2582169..2584184(+) (pilB/pilB1) [Nodularia spumigena UHCC 0039]
ATGACTTACTCCTCACCACAACGGCGCAGTACCGCTCTCACTACAAGAACAGAGTTTTCGCCCTTCGGCAACAAGCTAGT
ACAGTCTGGCTATGTCAATAGCGAACAGATGAGGCTAGCGCTGATTGAAAGCCGCAAGTCTGGCAAACCCTTGACAGAAG
TCTTGGAGTCTATTACTGGCAAACAACTAGCGCCTGAGTTTCTCAGACAATATAAAAAACAACAGCTCTTTGAGCTAAAA
ATACTCTATGGCGTTGAGTTCCTTGATCCAGAAATCAACCAAATTGGCAACCCGAAGGCCTGCAACCTGATTCACACCCT
GATCCCGGCGGATATTTGTCGTCGCCATCGCTTAGTGCCATTATCCAGACATGACGACCAAACCCCCCCCAGTGTCTTGG
TGGCAATGGTCGATCCGGATAATCTCGAAGCTTCCGATGACCTCAACCGCATCCTGCGCCCCCAAGGTTTGGCATTAAAA
TGCATGGTAATTACCCAAGAAGATTACCAACAGCTAATTAACCTATATTTGGATGATTTAGCTGTGCGGCAAAAGCAGCT
AGAAAAAGAAAAGTTTACAGATATTAATCAAGATTTGGAAAATCTTGATGATATCGACATAGACGATAGCTCTGAAGAGA
TGGAGGTTGATTTAGGGGTAGTCATGAAGGGTGCTGAAGATGCCCCAGTGATCAACCTTGTAAACAGAGTTCTAGCCAAA
GCCTTACATGAGGGTGTTTCTGATATTCACATTGAGCCACAGGAGGAAAATTTACGGATTCGGTTTCGTAAGGATGGGGT
ATTGAGTGAAGCTTTCGACCCCCTACCCAAAAAAATCGTGATGGCGGTAACAGCTCGCTTTAAAATCATCTCCAATCTAG
ACATTGCAGAGCGGCGCTTACCCCAAGATGGACGAATTAGGCGACTGTTCGAGGGACGGAAGGTGGACTTCCGGGTGAAT
ACCTTGCCTAGTCGCTATGGGGAAAAAGTAGTATTGCGGATTTTGGATAACTCTTCTACCCAGTTGGGATTAGATAAGTT
AATTACTGATCCGGAAACTTTGCAAATTGTTCAGGATATGGTCAGCCGTCCTTTTGGGTTGATTTTGGTAACTGGACCGA
CTGGTTCTGGTAAAACTACATCGCTATATTCGGCATTAGCAGAAAAGAACTCGCCGGGGATCAATATTAGTACAGTAGAA
GACCCCATTGAGTACAGTTTGCCAGGGATTACTCAAGTACAAGTAATTCGGGAAAAAGGGTTAGATTTTTCTACGGCTTT
GCGGGCGTTTTTGCGCCAAGACCCGGATGTATTGCTAGTGGGTGAAACACGGGACAAAGAAACTGCAAAAACCGCGATTG
AGGCTGCCTTAACTGGTCACTTAGTATTAACTACTTTACACACTAACGATGCCCCCGGTGCGATCGCTCGTCTGGGAGAA
ATGGGTATTGAGCCTTTTATGGTTTCTAGTTCCCTGATTGGTGTGTTAGCACAACGTTTGGTGCGGCGTGTCTGTTCTGA
ATGTCGTATTCCCTATACTCCCACAACTGAAGAACTCGCTCGCTATGGTCTATCAGCTTCCCAAAGCGCAAGTGTAACTT
TTTATAAAGCAAATACTTTAACATTAGGAGAAATTACGGAAGCGAAAGCCATAAATCAAGAGGTTTGTCCAAAGTGTAAT
GGTTCTGGCTACAAAGGACGTTGTGGTGTTTATGAAGTGATGCGAATCACGGAAAACCTGCAAACCTTGATTAACCAAGA
AGCACCCACAGAACGCATTAAGGAAGTGGCAGTAGAAGAAGGCATGAAAACCTTGCTGGCTTACAGTCTGGACTTAGTGC
GTCAGGGTGCTACGACTCTTGAAGAAGTCGAACGCGTCACCTTTACCGATACTGGGTTAGAAGCCGAGTTAAAAGCCAAA
CGCAAGAGTGGTCTTACTTGTGTAACTTGTAGTGCAATATTACAACCAGAATGGCTAGATTGTCCCTACTGCATGACACC
TCGTTTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.23

100

0.654

Multiple sequence alignment