Detailed information NTG0218657

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: XJ28_RS05525
• Genome accession: NZ_CP019871
• Coordinates: 1069236..1069667 (+)
• Length: 143 a.a.
• NCBI ID: WP_007246132.1
• Uniprot ID: A0AAW4E7G1
• Organism: Pseudomonas syringae pv. tomato strain B13-200
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1064236..1074667

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XJ28_RS05500 (XJ28_05410)	yacG	1064393..1064602 (-)	210	WP_003381207.1	DNA gyrase inhibitor YacG	-
XJ28_RS05505 (XJ28_05415)	coaE	1064599..1065222 (-)	624	WP_005770017.1	dephospho-CoA kinase	-
XJ28_RS05510 (XJ28_05420)	pilD	1065219..1066091 (-)	873	WP_007246135.1	prepilin peptidase	Machinery gene
XJ28_RS05515 (XJ28_05425)	pilC	1066093..1067310 (-)	1218	WP_007246134.1	type II secretion system F family protein	Machinery gene
XJ28_RS05520 (XJ28_05430)	pilB	1067313..1069007 (-)	1695	WP_007246133.1	type IV-A pilus assembly ATPase PilB	Machinery gene
XJ28_RS05525 (XJ28_05435)	pilA2	1069236..1069667 (+)	432	WP_007246132.1	pilin	Machinery gene
XJ28_RS05530 (XJ28_05440)	-	1069948..1070304 (-)	357	WP_007246131.1	winged helix-turn-helix transcriptional regulator	-
XJ28_RS05535 (XJ28_05445)	gstA	1070463..1071083 (+)	621	WP_007246130.1	glutathione transferase GstA	-
XJ28_RS05540 (XJ28_05450)	-	1071429..1072016 (+)	588	WP_010208432.1	DUF433 domain-containing protein	-
XJ28_RS05545 (XJ28_05455)	-	1072013..1072420 (+)	408	WP_007246127.1	hypothetical protein	-
XJ28_RS05550 (XJ28_05460)	-	1072465..1073010 (-)	546	WP_003383227.1	DUF1523 family protein	-
XJ28_RS05555 (XJ28_05465)	-	1073131..1074129 (+)	999	WP_007246126.1	ATP-grasp domain-containing protein	-

Sequence

Protein

• Length: 143 a.a.
• Molecular weight: 14498.75 Da
• Isoelectric Point: 9.1226

>NTDB_id=218657 XJ28_RS05525 WP_007246132.1 1069236..1069667(+) (pilA2) [Pseudomonas syringae pv. tomato strain B13-200]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRSKVTEGLTQAASAKLAVAETAANVGGVANVTAANSGFVFNPVAG
TNSYVGAVAVGAGGVITVTTKETGAAVQPVFNLNPVQASPNDQITWTCVRTAGLAKHLPANCR

Nucleotide

• Length: 432 bp

>NTDB_id=218657 XJ28_RS05525 WP_007246132.1 1069236..1069667(+) (pilA2) [Pseudomonas syringae pv. tomato strain B13-200]
ATGAAAGCACAAAAAGGTTTTACGTTGATCGAACTGATGATCGTGGTCGCGATCATCGGTATTTTGGCTGCGGTGGCACT
GCCTGCGTATCAGGATTACACAATCAGAAGTAAGGTTACTGAAGGTCTGACACAGGCGGCGTCGGCGAAGCTGGCAGTTG
CCGAGACTGCTGCGAATGTCGGCGGTGTAGCTAACGTAACGGCGGCGAACTCCGGGTTTGTGTTCAACCCAGTGGCAGGC
ACAAATTCATACGTTGGTGCCGTGGCTGTTGGTGCGGGTGGCGTTATCACTGTAACCACTAAGGAAACCGGAGCGGCGGT
CCAGCCTGTGTTCAACCTAAACCCGGTGCAAGCTTCACCCAACGATCAGATAACCTGGACTTGCGTGAGGACTGCTGGCC
TTGCCAAGCACCTTCCTGCTAACTGCCGGTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (1-27)

• Pilin (bacterial filament) (Pilin) (36-143)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	57.447	98.601	0.566
pilA2	Legionella pneumophila strain ERS1305867	57.447	98.601	0.566
pilA	Ralstonia pseudosolanacearum GMI1000	41.765	100	0.497
comP	Acinetobacter baylyi ADP1	43.791	100	0.469
pilE	Neisseria gonorrhoeae MS11	40.506	100	0.448
pilE	Neisseria gonorrhoeae strain FA1090	39.49	100	0.434
pilA/pilA1	Eikenella corrodens VA1	39.103	100	0.427
pilA/pilAI	Pseudomonas stutzeri DSM 10701	40.69	100	0.413
pilA	Acinetobacter baumannii strain A118	39.583	100	0.399
pilA	Glaesserella parasuis strain SC1401	36.735	100	0.378
pilA/pilAII	Pseudomonas stutzeri DSM 10701	38.849	97.203	0.378