Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   XJ28_RS05515 Genome accession   NZ_CP019871
Coordinates   1066093..1067310 (-) Length   405 a.a.
NCBI ID   WP_007246134.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. tomato strain B13-200     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1061093..1072310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XJ28_RS05475 (XJ28_05395) - 1062444..1062977 (-) 534 WP_005770014.1 MOSC domain-containing protein -
  XJ28_RS05480 (XJ28_05400) - 1062974..1063603 (-) 630 WP_007246137.1 DUF1780 domain-containing protein -
  XJ28_RS05490 (XJ28_05405) - 1063800..1064126 (+) 327 WP_007246136.1 hypothetical protein -
  XJ28_RS05500 (XJ28_05410) yacG 1064393..1064602 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  XJ28_RS05505 (XJ28_05415) coaE 1064599..1065222 (-) 624 WP_005770017.1 dephospho-CoA kinase -
  XJ28_RS05510 (XJ28_05420) pilD 1065219..1066091 (-) 873 WP_007246135.1 prepilin peptidase Machinery gene
  XJ28_RS05515 (XJ28_05425) pilC 1066093..1067310 (-) 1218 WP_007246134.1 type II secretion system F family protein Machinery gene
  XJ28_RS05520 (XJ28_05430) pilB 1067313..1069007 (-) 1695 WP_007246133.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XJ28_RS05525 (XJ28_05435) pilA2 1069236..1069667 (+) 432 WP_007246132.1 pilin Machinery gene
  XJ28_RS05530 (XJ28_05440) - 1069948..1070304 (-) 357 WP_007246131.1 winged helix-turn-helix transcriptional regulator -
  XJ28_RS05535 (XJ28_05445) gstA 1070463..1071083 (+) 621 WP_007246130.1 glutathione transferase GstA -
  XJ28_RS05540 (XJ28_05450) - 1071429..1072016 (+) 588 WP_010208432.1 DUF433 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44163.16 Da        Isoelectric Point: 10.2041

>NTDB_id=218655 XJ28_RS05515 WP_007246134.1 1066093..1067310(-) (pilC) [Pseudomonas syringae pv. tomato strain B13-200]
MASKVVKLSVYTWEGVDKKGGKLSGELSGHNPALIKAQLRKQGVNPTKVRKKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRSLVNSLKQEVSAGNSFATALRQKPEHFDDLFCNLVDAGEQAGALESLLDRVATYKEKT
EKLKAKIKKAMTYPAAVLIVAVIVSGILLIKVVPQFQSVFAGFGAELPMFTLMVISLSEVIQQWWIAIVVAFFASFFLFK
KAYKKSQKFRDRIDRLLLKLPIIGPLIFKSSVARYARTLATTFSAGVPLVEALESVAGATGNVVFKNAVHKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMAILGVVVGGLVIAMYLPIFK
LGEVA

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=218655 XJ28_RS05515 WP_007246134.1 1066093..1067310(-) (pilC) [Pseudomonas syringae pv. tomato strain B13-200]
ATGGCCAGCAAGGTAGTCAAACTCAGCGTCTACACTTGGGAAGGTGTAGACAAAAAAGGCGGCAAACTTAGCGGAGAATT
GAGCGGCCACAACCCTGCGCTTATCAAAGCACAATTACGTAAACAGGGTGTCAATCCAACCAAGGTGCGCAAGAAATCGG
TTTCAATCTTCGGCAAAGGCAAGAAGATCAAACCACTGGATATCGCATTTTTCTCTCGACAAATGGCCACTATGATGAAG
GCAGGTGTACCATTGCTGCAATCATTCGACATTATCAGCGAGGGTGCAGAAAACCCGAACATGCGTTCACTTGTAAACTC
ACTAAAGCAAGAAGTTTCGGCAGGTAACAGCTTTGCAACTGCCTTACGACAAAAGCCTGAGCATTTTGATGACCTGTTTT
GTAATCTAGTAGATGCGGGGGAACAGGCAGGTGCGCTCGAAAGCCTGCTGGACCGTGTAGCCACCTATAAAGAAAAAACG
GAAAAGCTCAAAGCAAAGATCAAGAAAGCAATGACTTATCCTGCTGCCGTTTTAATCGTCGCGGTCATCGTCTCAGGCAT
TTTGCTCATAAAAGTCGTACCACAATTCCAGTCGGTATTTGCGGGCTTTGGCGCTGAGCTACCAATGTTTACATTGATGG
TCATCAGCCTCTCTGAGGTCATACAGCAATGGTGGATAGCTATTGTCGTGGCATTTTTCGCTAGCTTTTTTTTATTCAAA
AAAGCCTATAAAAAATCACAGAAATTTCGTGACCGCATCGACCGACTATTGCTGAAACTACCAATCATTGGACCGCTGAT
TTTCAAATCTTCAGTAGCTCGCTATGCACGGACACTGGCAACCACCTTTTCCGCAGGCGTCCCACTTGTAGAAGCTCTGG
AATCGGTAGCGGGTGCGACCGGCAATGTGGTTTTCAAAAACGCAGTACATAAGGTAAAACAAGATGTCTCTACAGGCATG
CAATTAAATTTCTCGATGAGATCTACAGGAGTTTTTCCAAGCCTGGCGATTCAAATGACTGCTATCGGTGAAGAGTCAGG
TGCACTGGACAGCATGTTGGATAAAGTCGCGACTTACTACGAGGATGAAGTCGATAACATGGTGGATAGCCTGACTAGCC
TGATGGAACCAATGATAATGGCCATACTTGGCGTGGTAGTTGGCGGGCTAGTGATAGCGATGTATCTGCCTATCTTCAAG
CTTGGAGAAGTTGCCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.02

99.753

0.728
  pilC Acinetobacter baumannii D1279779

60.902

98.519

0.6
  pilC Acinetobacter baylyi ADP1

59.259

100

0.593
  pilC Legionella pneumophila strain ERS1305867

52.273

97.778

0.511
  pilC Vibrio cholerae strain A1552

42.327

99.753

0.422
  pilC Vibrio campbellii strain DS40M4

42.607

98.519

0.42
  pilG Neisseria gonorrhoeae MS11

42.025

97.531

0.41
  pilG Neisseria meningitidis 44/76-A

41.772

97.531

0.407
  pilC Thermus thermophilus HB27

38.958

99.506

0.388

Multiple sequence alignment