Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   MS8345_RS11525 Genome accession   NZ_CP025401
Coordinates   2161823..2163250 (+) Length   475 a.a.
NCBI ID   WP_001513843.1    Uniprot ID   -
Organism   Escherichia coli strain MS8345     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2156823..2168250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS8345_RS11490 (MS8345_02123) yeeW 2157007..2157201 (+) 195 WP_000988600.1 DUF5983 family protein -
  MS8345_RS30050 - 2157247..2157327 (+) 81 Protein_2143 hypothetical protein -
  MS8345_RS11495 - 2157616..2157744 (-) 129 Protein_2144 transposase domain-containing protein -
  MS8345_RS11500 - 2157816..2157998 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  MS8345_RS11505 (MS8345_02124) tmaR 2158099..2158428 (-) 330 WP_000450409.1 DUF496 family protein -
  MS8345_RS11510 (MS8345_02125) yeeA 2158600..2159658 (-) 1059 WP_001200888.1 FUSC family protein -
  MS8345_RS11515 (MS8345_02126) sbmC 2159856..2160329 (-) 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
  MS8345_RS11520 (MS8345_02127) dacD 2160448..2161614 (-) 1167 WP_001513842.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  MS8345_RS11525 (MS8345_02128) sbcB 2161823..2163250 (+) 1428 WP_001513843.1 exodeoxyribonuclease I Machinery gene
  MS8345_RS11530 (MS8345_02129) tsuB 2163293..2163520 (-) 228 WP_083574903.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  MS8345_RS11535 (MS8345_02130) tsuA 2163534..2164592 (-) 1059 WP_000492340.1 thiosulfate utilization transporter TsuA/YeeE -
  MS8345_RS11540 (MS8345_02131) plaP 2164771..2166129 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  MS8345_RS11545 yoeI 2166119..2166181 (-) 63 WP_010723108.1 membrane protein YoeI -
  MS8345_RS11550 (MS8345_02132) yeeY 2166396..2167325 (-) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  MS8345_RS11555 (MS8345_02133) yeeZ 2167371..2168195 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54426.73 Da        Isoelectric Point: 5.4720

>NTDB_id=218656 MS8345_RS11525 WP_001513843.1 2161823..2163250(+) (sbcB) [Escherichia coli strain MS8345]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFAPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=218656 MS8345_RS11525 WP_001513843.1 2161823..2163250(+) (sbcB) [Escherichia coli strain MS8345]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTCTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAACTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGCGCAGCAATGAAAATTGTGCTTGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAGCTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCGCGCCAGAGTTTTTGCAGGGTTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGT
ATGCCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581

Multiple sequence alignment