Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XJ28_RS05520 Genome accession   NZ_CP019871
Coordinates   1067313..1069007 (-) Length   564 a.a.
NCBI ID   WP_007246133.1    Uniprot ID   A0AAW4E716
Organism   Pseudomonas syringae pv. tomato strain B13-200     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1062313..1074007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XJ28_RS05475 (XJ28_05395) - 1062444..1062977 (-) 534 WP_005770014.1 MOSC domain-containing protein -
  XJ28_RS05480 (XJ28_05400) - 1062974..1063603 (-) 630 WP_007246137.1 DUF1780 domain-containing protein -
  XJ28_RS05490 (XJ28_05405) - 1063800..1064126 (+) 327 WP_007246136.1 hypothetical protein -
  XJ28_RS05500 (XJ28_05410) yacG 1064393..1064602 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  XJ28_RS05505 (XJ28_05415) coaE 1064599..1065222 (-) 624 WP_005770017.1 dephospho-CoA kinase -
  XJ28_RS05510 (XJ28_05420) pilD 1065219..1066091 (-) 873 WP_007246135.1 prepilin peptidase Machinery gene
  XJ28_RS05515 (XJ28_05425) pilC 1066093..1067310 (-) 1218 WP_007246134.1 type II secretion system F family protein Machinery gene
  XJ28_RS05520 (XJ28_05430) pilB 1067313..1069007 (-) 1695 WP_007246133.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XJ28_RS05525 (XJ28_05435) pilA2 1069236..1069667 (+) 432 WP_007246132.1 pilin Machinery gene
  XJ28_RS05530 (XJ28_05440) - 1069948..1070304 (-) 357 WP_007246131.1 winged helix-turn-helix transcriptional regulator -
  XJ28_RS05535 (XJ28_05445) gstA 1070463..1071083 (+) 621 WP_007246130.1 glutathione transferase GstA -
  XJ28_RS05540 (XJ28_05450) - 1071429..1072016 (+) 588 WP_010208432.1 DUF433 domain-containing protein -
  XJ28_RS05545 (XJ28_05455) - 1072013..1072420 (+) 408 WP_007246127.1 hypothetical protein -
  XJ28_RS05550 (XJ28_05460) - 1072465..1073010 (-) 546 WP_003383227.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62413.67 Da        Isoelectric Point: 6.3854

>NTDB_id=218656 XJ28_RS05520 WP_007246133.1 1067313..1069007(-) (pilB) [Pseudomonas syringae pv. tomato strain B13-200]
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAELASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETSLAAQNDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIREGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=218656 XJ28_RS05520 WP_007246133.1 1067313..1069007(-) (pilB) [Pseudomonas syringae pv. tomato strain B13-200]
ATGACTGATGCTGTCCTCACTGGCCTGGCCAAACAATTGGTCCTCGCCGAGCTTCTTACTGAACCTGTTGCGCAGCAAGC
GTATCAACAGGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGAACAAACTGGTCAAAAGCCTGACACTGG
CGGAATTGGCTTCTGATCAGTTCGGCGTTCCGTTCATGGACCTCGCCAGTCTGGACAAGGAAAGCCAGCCCAAAGGCCTG
GTGAGTGAAAAACTGGTTCGGCAACATCATGCACTGCCGCTGTGGCGTCGCGGTAACAAGCTATTTATCGGGATTTCCGA
CCCGACCAATCATCAGGCGGTCACTGACATACAGTTCAGCACCGGCCTGAATACCGAAGCGATTCTGGTCGAAGACGACA
AGCTGACCACTGCAATAGATCGTTTTTTTGACAGTGATAGCGGTCTGGGTAATCTGGAAGATGTCGATCTGGGTCTGGAT
ATCGAACCAGCTGATGGCAAGGAAACCTCCCTCGCCGCGCAGAATGACGCCGACGACGCCCCAGTGGTCCGTTTCGTCAA
TAAAATGCTGCTGGATGCAATCCGGCTAGGATCATCCGATTTACATTTCGAACCCTACGAAAAGATCTTTCGCGTGCGCT
TACGCACCGACGGCATTTTGCATGAAGTTGCCAGGCCGCCGATTCATCTGGCAAACCGTATTGCTGCACGTTTGAAAGTT
ATGGCCAGCCTCGACATTTCGGAACGGCGCAAACCGCAGGACGGTCGGGTCAAGTTGCGGGTGTCCAAAACAAAGGCCAT
CGATTTTCGTATGAACACCTTGCCCACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCGACCAGTGCGCAGA
TGGGTATTGATGCTCTGGGCTACGAGCCGGAGCAAAAAGCACTGTATCTGGAGGCCCTGAAACAACCGCAGGGCATGATT
CTTGTGACTGGCCCCACGGGTTCCGGCAAGACCGTTTCTCTGTATACCGGTCTGAACATACTCAATACCGTGGACATCAA
CATTTCTACAGCCGAAGACCCGGTAGAGATCAACCTGGAAGGTATCAATCAGGTCAACGTCAACCCACGCCAAGGATTGG
ACTTTTCGCAGGCATTGCGCGCTTTCCTGCGTCAAGATCCTGACGTGATCATGGTCGGCGAGATTCGCGACTTGGAAACC
GCCGAAATCGCCATCAAGGCGTCACAGACCGGCCACATGGTGCTGTCCACACTGCACACCAACAGCGCGGCAGAAACCCT
GACTCGCCTGCATCATATGGGCGTAGCCGCATTCAACATTGCCACAGCAATCAATCTGATTATTGCGCAACGGCTCGCGC
GCAAGCTGTGCAGCCATTGCAAGAAAGAACTCGATATTCCTCGCGAGACACTGATTCGGGAAGGTTTTCCAGAAGCGAAG
ATTGGCACTTTCAAGATCTATGGTCCGGTGGGCTGCGAACATTGCAACGGTGGCTATAGAGGCAGGGTCGGTATCTACGA
AGTCGTCAAGAAAACAGCAGAGCTGGAGCGCATCATCATGGAAGAGGGTAATTCACTGGAGATTTCCAGGCAGATGCGCA
AGGACGGTTTCAACGACCTGCGAACCTCGGGCCTGTCAAAAGCAATGCAGGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.597

100

0.578
  pilB Acinetobacter baylyi ADP1

56.537

100

0.567
  pilB Legionella pneumophila strain ERS1305867

55.929

100

0.56
  pilB Vibrio cholerae strain A1552

50.89

99.645

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.626

99.113

0.502
  pilB Vibrio campbellii strain DS40M4

49.47

100

0.496
  pilF Neisseria gonorrhoeae MS11

48.221

99.645

0.481
  pilF Thermus thermophilus HB27

39.046

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.973

93.262

0.363

Multiple sequence alignment