Detailed information NTG0218654

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: XJ28_RS05510
• Genome accession: NZ_CP019871
• Coordinates: 1065219..1066091 (-)
• Length: 290 a.a.
• NCBI ID: WP_007246135.1
• Uniprot ID: A0AAW4E6R8
• Organism: Pseudomonas syringae pv. tomato strain B13-200
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1060219..1071091

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XJ28_RS05465 (XJ28_05385)	-	1060929..1062227 (-)	1299	WP_005614782.1	NAD(P)/FAD-dependent oxidoreductase	-
XJ28_RS05475 (XJ28_05395)	-	1062444..1062977 (-)	534	WP_005770014.1	MOSC domain-containing protein	-
XJ28_RS05480 (XJ28_05400)	-	1062974..1063603 (-)	630	WP_007246137.1	DUF1780 domain-containing protein	-
XJ28_RS05490 (XJ28_05405)	-	1063800..1064126 (+)	327	WP_007246136.1	hypothetical protein	-
XJ28_RS05500 (XJ28_05410)	yacG	1064393..1064602 (-)	210	WP_003381207.1	DNA gyrase inhibitor YacG	-
XJ28_RS05505 (XJ28_05415)	coaE	1064599..1065222 (-)	624	WP_005770017.1	dephospho-CoA kinase	-
XJ28_RS05510 (XJ28_05420)	pilD	1065219..1066091 (-)	873	WP_007246135.1	prepilin peptidase	Machinery gene
XJ28_RS05515 (XJ28_05425)	pilC	1066093..1067310 (-)	1218	WP_007246134.1	type II secretion system F family protein	Machinery gene
XJ28_RS05520 (XJ28_05430)	pilB	1067313..1069007 (-)	1695	WP_007246133.1	type IV-A pilus assembly ATPase PilB	Machinery gene
XJ28_RS05525 (XJ28_05435)	pilA2	1069236..1069667 (+)	432	WP_007246132.1	pilin	Machinery gene
XJ28_RS05530 (XJ28_05440)	-	1069948..1070304 (-)	357	WP_007246131.1	winged helix-turn-helix transcriptional regulator	-
XJ28_RS05535 (XJ28_05445)	gstA	1070463..1071083 (+)	621	WP_007246130.1	glutathione transferase GstA	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31995.21 Da
• Isoelectric Point: 8.1603

>NTDB_id=218654 XJ28_RS05510 WP_007246135.1 1065219..1066091(-) (pilD) [Pseudomonas syringae pv. tomato strain B13-200]
MPLLDLLASSPLAFVTTCCLLGLIVGSFLNVVVYRLPKMMERDWKVQSREMLGLPAEPDQPVFNLILPHSSCPHCAHKIR
PWENLPVISYLLLRGKCSHCKASISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLSDALWGAVAGYLTLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=218654 XJ28_RS05510 WP_007246135.1 1065219..1066091(-) (pilD) [Pseudomonas syringae pv. tomato strain B13-200]
ATGCCCCTCCTCGACCTCTTGGCCAGCTCTCCCCTGGCCTTCGTCACCACCTGCTGCCTACTTGGCCTGATCGTCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCCTCCCAAAAATGATGGAGCGCGACTGGAAAGTACAATCACGGGAAATGCTAGGCC
TCCCTGCCGAACCGGACCAACCCGTCTTCAACCTGATCCTGCCGCACTCAAGCTGCCCGCACTGCGCGCACAAGATCCGC
CCATGGGAAAACTTGCCGGTCATCAGCTACCTGTTGCTGCGGGGCAAATGCTCACACTGCAAAGCCTCCATCAGCAAGCG
CTACCCGTTGGTAGAACTGACCTGCGCAGTGTTGTCGGCCTACGTCGCCTGGCATTTCGGCTTCGGCTGGCAGGCGGCTG
CGATGCTGGTGTTGAGCTGGGGTTTGCTGGCGATGAGTCTGATCGATGCCGATCATCAGCTGTTACCTGACTCTTTGGTG
CTGCCACTGCTTTGGCTGGGCCTGATCGTTAACGCCTTTGGGCTGTTCACCTCGCTGAGCGACGCGCTGTGGGGTGCAGT
GGCCGGTTATCTGACGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACGGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCGTGGGGTGGCTGGCAGATTCTGCCGTTGACCATTCTGCTGTCGTCACTGGTGGGT
GCGGTACTTGGGGTGATCATGATGCGCGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTATATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.417	97.586	0.531
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	51.825	94.483	0.49
pilD	Neisseria gonorrhoeae MS11	52.239	92.414	0.483