Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   XPE_RS18375 Genome accession   NZ_CP019725
Coordinates   4100766..4101158 (+) Length   130 a.a.
NCBI ID   WP_008576871.1    Uniprot ID   A0A6P0G6X8
Organism   Xanthomonas perforans 91-118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4100766..4110229 4100766..4101158 within 0


Gene organization within MGE regions


Location: 4100766..4110229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XPE_RS18375 (XPE_18320) pilA/pilAI 4100766..4101158 (+) 393 WP_008576871.1 pilin Machinery gene
  XPE_RS18380 (XPE_18325) - 4101251..4101670 (+) 420 WP_033479197.1 pilin -
  XPE_RS18385 (XPE_18330) - 4101715..4103547 (+) 1833 WP_008576878.1 hypothetical protein -
  XPE_RS18390 (XPE_18335) - 4103669..4104367 (+) 699 WP_158070652.1 class I SAM-dependent methyltransferase -
  XPE_RS22190 (XPE_18340) - 4104385..4105278 (+) 894 WP_074053366.1 lysylphosphatidylglycerol synthase domain-containing protein -
  XPE_RS18395 - 4105270..4106436 (-) 1167 WP_077708931.1 glycosyltransferase -
  XPE_RS18400 (XPE_18350) - 4106429..4107286 (-) 858 WP_074053368.1 glycosyltransferase family 2 protein -
  XPE_RS18405 (XPE_18355) pilB 4107543..4109279 (+) 1737 WP_008575094.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 130 a.a.        Molecular weight: 13565.77 Da        Isoelectric Point: 9.5775

>NTDB_id=218104 XPE_RS18375 WP_008576871.1 4100766..4101158(+) (pilA/pilAI) [Xanthomonas perforans 91-118]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQVTAGLAEITPGKTQFEVKTNEGSTMSTPAQIGLSSSTTRCGI
TVSATTIVCNLKGNAAKVKDKNVTLTRTASTGTWACSSSVDAKYKPAGCS

Nucleotide


Download         Length: 393 bp        

>NTDB_id=218104 XPE_RS18375 WP_008576871.1 4100766..4101158(+) (pilA/pilAI) [Xanthomonas perforans 91-118]
ATGAAGAAGCAGCAAGGCTTTACGCTGATCGAACTGATGATCGTGGTCGCGATCATTGCCATCCTGGCCGCCATCGCGCT
GCCGATGTATCAGGACTATGTCGCCAAGTCGCAGGTGACTGCTGGTCTGGCCGAAATCACCCCGGGCAAGACTCAGTTCG
AAGTTAAGACCAACGAAGGCAGCACGATGAGCACCCCCGCGCAGATTGGTCTAAGCTCGTCCACCACGCGTTGTGGCATC
ACGGTTTCCGCTACTACCATTGTTTGCAACCTTAAAGGAAACGCGGCAAAGGTCAAGGATAAGAACGTGACACTGACGCG
TACCGCTAGCACGGGAACTTGGGCTTGCAGCAGCAGTGTTGACGCGAAGTACAAGCCGGCTGGCTGCAGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P0G6X8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

55.556

100

0.577
  pilA Acinetobacter baumannii strain A118

46.763

100

0.5
  pilA/pilAII Pseudomonas stutzeri DSM 10701

47.368

100

0.485
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.056

100

0.477
  pilA Vibrio cholerae strain A1552

43.056

100

0.477
  pilA Vibrio cholerae C6706

43.056

100

0.477
  pilA Pseudomonas aeruginosa PAK

41.611

100

0.477
  comP Acinetobacter baylyi ADP1

40.541

100

0.462
  pilA2 Legionella pneumophila str. Paris

37.931

100

0.423
  pilA2 Legionella pneumophila strain ERS1305867

37.931

100

0.423
  pilA/pilA1 Eikenella corrodens VA1

36.242

100

0.415
  pilA Vibrio parahaemolyticus RIMD 2210633

42.742

95.385

0.408
  pilE Neisseria gonorrhoeae strain FA1090

30.719

100

0.362

Multiple sequence alignment