Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XPE_RS18405 Genome accession   NZ_CP019725
Coordinates   4107543..4109279 (+) Length   578 a.a.
NCBI ID   WP_008575094.1    Uniprot ID   -
Organism   Xanthomonas perforans 91-118     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4100766..4110229 4107543..4109279 within 0


Gene organization within MGE regions


Location: 4100766..4110229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XPE_RS18375 (XPE_18320) pilA/pilAI 4100766..4101158 (+) 393 WP_008576871.1 pilin Machinery gene
  XPE_RS18380 (XPE_18325) - 4101251..4101670 (+) 420 WP_033479197.1 pilin -
  XPE_RS18385 (XPE_18330) - 4101715..4103547 (+) 1833 WP_008576878.1 hypothetical protein -
  XPE_RS18390 (XPE_18335) - 4103669..4104367 (+) 699 WP_158070652.1 class I SAM-dependent methyltransferase -
  XPE_RS22190 (XPE_18340) - 4104385..4105278 (+) 894 WP_074053366.1 lysylphosphatidylglycerol synthase domain-containing protein -
  XPE_RS18395 - 4105270..4106436 (-) 1167 WP_077708931.1 glycosyltransferase -
  XPE_RS18400 (XPE_18350) - 4106429..4107286 (-) 858 WP_074053368.1 glycosyltransferase family 2 protein -
  XPE_RS18405 (XPE_18355) pilB 4107543..4109279 (+) 1737 WP_008575094.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62706.95 Da        Isoelectric Point: 5.5986

>NTDB_id=218105 XPE_RS18405 WP_008575094.1 4107543..4109279(+) (pilB) [Xanthomonas perforans 91-118]
MTATLTANLVGITGIARRLVQDGALDEVAARAAMEHASLAKIPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSALG
DDDEGMDNLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=218105 XPE_RS18405 WP_008575094.1 4107543..4109279(+) (pilB) [Xanthomonas perforans 91-118]
ATGACCGCAACACTCACCGCAAACTTGGTTGGTATCACTGGCATCGCCCGGCGACTGGTTCAGGACGGCGCGCTTGACGA
AGTCGCGGCGCGCGCTGCGATGGAGCACGCGAGTTTGGCGAAAATTCCTTTGCCACAGTGGTTCGCAGAAAAAAAACTCG
TGACGGCGTCTCAGCTGGCCGCTGCAAATGCAGTCGAGTTCGGCATGCCGTTGATGGACGTATCGGTGTTCGATGCTAAT
CAAAATGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGTAACCGGCT
GTTCGTGGGGGTGAGCAACCCGACCCAGACTCGCGCGCTAGACGACATCAAGTTCCATACGAATCTCGTGGTCGAGCCCA
TCCTGGTCGATGAGGACCAGATCCGTCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCTGCGCTGGGATCTGCGCTGGGT
GACGACGACGAAGGTATGGACAACCTAGACGTGTCTGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCCTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGACTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAAGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTTGGAATTCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTTGCCGCAGCGTTGCGCTCGTTCCTACGCCAGGACCCG
GACATCATCATGGTTGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATTGCACGCTTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACCTTGGTGATCGCGCAACGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGATTCACCCCCGCGCAGCTTGCTGCCGGGATCGAGCTGTATGAAGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAGGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCAGCCTTGATG
AAGGCTGCCCACGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.634

98.27

0.547
  pilB Acinetobacter baumannii D1279779

55.36

98.443

0.545
  pilB Legionella pneumophila strain ERS1305867

51.845

98.443

0.51
  pilB Vibrio cholerae strain A1552

48.966

100

0.491
  pilF Neisseria gonorrhoeae MS11

48.415

98.27

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

46.786

96.886

0.453
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448

Multiple sequence alignment