Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BWP24_RS02325 Genome accession   NZ_CP019293
Coordinates   472543..473649 (+) Length   368 a.a.
NCBI ID   WP_005425812.1    Uniprot ID   A7MTP8
Organism   Vibrio campbellii strain LMB29     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 467543..478649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWP24_RS02290 (BWP24_02330) - 467683..468285 (-) 603 WP_005436525.1 XTP/dITP diphosphatase -
  BWP24_RS02295 (BWP24_02335) - 468391..468822 (-) 432 WP_005425804.1 DUF4426 domain-containing protein -
  BWP24_RS02300 (BWP24_02340) yggU 468882..469172 (-) 291 WP_005425805.1 DUF167 family protein YggU -
  BWP24_RS02305 (BWP24_02345) - 469172..469729 (-) 558 WP_005425806.1 YggT family protein -
  BWP24_RS02310 (BWP24_02350) proC 469783..470601 (-) 819 WP_077200190.1 pyrroline-5-carboxylate reductase -
  BWP24_RS02315 (BWP24_02355) - 470732..471442 (-) 711 WP_077200191.1 YggS family pyridoxal phosphate-dependent enzyme -
  BWP24_RS02320 (BWP24_02360) pilT 471471..472511 (+) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  BWP24_RS02325 (BWP24_02365) pilU 472543..473649 (+) 1107 WP_005425812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BWP24_RS02330 (BWP24_02370) ruvX 473711..474136 (-) 426 WP_005425820.1 Holliday junction resolvase RuvX -
  BWP24_RS02335 (BWP24_02375) - 474184..474747 (-) 564 WP_010450344.1 YqgE/AlgH family protein -
  BWP24_RS02340 (BWP24_02380) gshB 474876..475826 (-) 951 WP_077200192.1 glutathione synthase -
  BWP24_RS02345 (BWP24_02385) rsmE 475841..476572 (-) 732 WP_045384524.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BWP24_RS02350 (BWP24_02390) endA 476719..477414 (-) 696 WP_077200193.1 deoxyribonuclease I -
  BWP24_RS02355 (BWP24_02395) - 477564..478061 (-) 498 WP_045395412.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41335.48 Da        Isoelectric Point: 6.7731

>NTDB_id=214632 BWP24_RS02325 WP_005425812.1 472543..473649(+) (pilU) [Vibrio campbellii strain LMB29]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVALLLDSAMEPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=214632 BWP24_RS02325 WP_005425812.1 472543..473649(+) (pilU) [Vibrio campbellii strain LMB29]
ATGGATCTGAATAAATTTCTTGAAGGCATGTTGGCGTTAAAAGCGTCGGATCTCTACATCACTGTCGGTGCTCCGATTTT
GTTTCGTGTGGATGGCGAATTGCGCCCGCAAGGTGATAAGCTGACTGAAAATGATGTCGCCTTGTTACTCGACAGTGCCA
TGGAACCAGAGCGACGCCAGGAGTTTCGTAAAAGCCGTGAGTCTAACTTTGCCATTGTCCGTGATTGCGGTCGTTTCCGT
GTCAGTGCCTTTTTCCAACGTGAATTACCGGGAGCCGTTATTCGTCGTATTGAAACCAATATTCCGACGTTTGAGCAACT
TAAGTTACCTTTGGTACTGCAAGATCTTGCTATTGCCAAGCGTGGTTTAGTGCTGGTGGTTGGTGCCACTGGTTCTGGTA
AGTCGACCACTATGGCGGCGATGACGGGTTACCGCAACAACAACAAAACGGGTCATATCCTGACGGTAGAAGACCCGATT
GAATTTGTGCACGAACATAAGCGTTGCATCGTGACACAGCGTGAAGTTGGCTTAGACACCGACAGCTATGAAGTGGCGTT
GAAAAACTCGTTACGTCAGGCGCCAGATATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTTGCTGAGACTGGTCACTTGTGTATGGCGACACTGCACGCCAACAACGCAAACCAAGCGCTTGAGCGTATTTTGCAC
CTAGTGCCGAAAGATCAGAAAGATCAGTTCTTATTTGATCTGTCGATGAACTTAAAAGGTGTGGTGGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAACAGTCCACGTGTTTCTGATCTGATCCGTC
GAGGTGATCTGCATGAATTGAAATCGACCATGGCTCGCTCTAACGAGTTCGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTGGTGATGCAAGGTAAGATCAGCGAAGAGGATGCATTGCACAGTGCAGATTCAGCCAACGATCTACGTTTAATGTT
GAAGACGCAACGTGGTGAAGCCTTCTCTACTGGCACGCTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7MTP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.25

100

0.813
  pilU Pseudomonas stutzeri DSM 10701

58.757

96.196

0.565
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.86

93.478

0.391
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Pseudomonas stutzeri DSM 10701

40.936

92.935

0.38
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375

Multiple sequence alignment