Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MCA_RS07575 Genome accession   NC_002977
Coordinates   1639147..1640265 (+) Length   372 a.a.
NCBI ID   WP_010960810.1    Uniprot ID   Q608F4
Organism   Methylococcus capsulatus str. Bath     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1634147..1645265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCA_RS07555 (MCA1534) - 1635875..1636453 (-) 579 WP_010960806.1 YggT family protein -
  MCA_RS07560 (MCA1535) proC 1636457..1637281 (-) 825 WP_010960807.1 pyrroline-5-carboxylate reductase -
  MCA_RS07565 (MCA1536) - 1637352..1638041 (-) 690 WP_010960808.1 YggS family pyridoxal phosphate-dependent enzyme -
  MCA_RS07570 (MCA1537) pilT 1638097..1639131 (+) 1035 WP_010960809.1 type IV pilus twitching motility protein PilT Machinery gene
  MCA_RS07575 (MCA1538) pilU 1639147..1640265 (+) 1119 WP_010960810.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MCA_RS07580 - 1640471..1640755 (+) 285 WP_041361062.1 hypothetical protein -
  MCA_RS07585 (MCA1539) - 1640804..1642597 (-) 1794 WP_010960811.1 VWA domain-containing protein -
  MCA_RS07590 (MCA1540) - 1642608..1643030 (-) 423 WP_010960812.1 protein YgfX -
  MCA_RS07595 (MCA1541) - 1643024..1643275 (-) 252 WP_010960813.1 succinate dehydrogenase assembly factor 2 -
  MCA_RS07600 (MCA1542) - 1643272..1644054 (-) 783 WP_010960814.1 succinate dehydrogenase iron-sulfur subunit -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41636.97 Da        Isoelectric Point: 6.7686

>NTDB_id=21448 MCA_RS07575 WP_010960810.1 1639147..1640265(+) (pilU) [Methylococcus capsulatus str. Bath]
MEFASLLRLMVLKKASDLFITAAKEPCMKLNGAIVPLSSTKLSTDQVRQLVLGIMNQRQRDEFENTNECNFALSAAGLGR
FRVSAFVQRNSPGMVLRRIETEIPTVEQLNLPSVLNDLVMTKRGLILFVGATGTGKSTSLAAMLKYRNEHSSGHIITIED
PLEFVHPHAGCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMQQAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHPQVFMDLSLNLRAIIAQQLVRRADGKGRYPAVEILINTPLVSDLIRQGEVHKLKDVMKQSREQGMQTFDQA
LFELFKAGRIGYEDALYSADSKNEVRLMIKLSEEGSIDKYAPKDDSIRIVDN

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=21448 MCA_RS07575 WP_010960810.1 1639147..1640265(+) (pilU) [Methylococcus capsulatus str. Bath]
ATGGAATTCGCATCGCTGCTCAGGCTGATGGTACTGAAGAAAGCGTCCGATCTCTTCATCACCGCGGCCAAGGAGCCCTG
CATGAAGCTGAACGGCGCCATCGTGCCGCTGTCCAGCACCAAACTCTCCACAGACCAGGTGCGCCAGCTCGTCCTCGGAA
TCATGAACCAGCGCCAGCGCGACGAGTTCGAGAACACCAACGAATGCAACTTCGCCCTCTCCGCGGCCGGACTGGGCCGG
TTCCGGGTCAGCGCCTTCGTGCAGCGGAACAGTCCCGGCATGGTGCTGCGCAGGATCGAGACCGAGATTCCCACCGTGGA
ACAGCTCAATCTGCCCTCCGTCCTCAACGACCTGGTCATGACCAAGCGCGGCCTGATCCTGTTCGTGGGCGCGACCGGCA
CCGGCAAGTCGACGTCGCTGGCGGCGATGCTCAAGTACCGCAACGAGCACTCCAGCGGCCACATCATCACCATCGAGGAT
CCCCTGGAATTCGTACACCCGCACGCGGGCTGCATCGTCACCCAGCGCGAGGTCGGCATCGATACCGAATCCTACGAAGT
CGCGCTGAAGAACACCCTGCGGCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGCACCCGCGAGACCATGCAGCAGG
CCATCACCTTCGCCGAAACCGGCCACCTCTGCCTGAGCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATTTCTTCCCGGAAGACATGCATCCGCAGGTGTTCATGGACCTGTCGCTGAACCTGCGTGCCATCATCGCCCAGCA
GCTCGTCAGGCGCGCCGACGGCAAGGGGCGCTATCCGGCGGTGGAAATCCTCATCAATACCCCTCTGGTCTCCGACCTGA
TCCGCCAGGGCGAGGTCCACAAGCTCAAGGACGTGATGAAACAGTCGCGCGAACAGGGCATGCAGACCTTCGACCAGGCG
CTGTTCGAATTGTTCAAGGCGGGCAGGATCGGCTACGAGGATGCCCTCTACTCCGCCGATTCCAAGAACGAAGTCCGCTT
GATGATCAAGCTCAGCGAAGAAGGCAGCATCGACAAATACGCGCCCAAGGACGACTCGATCCGGATCGTCGACAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q608F4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.774

95.161

0.664
  pilU Acinetobacter baylyi ADP1

64.706

95.968

0.621
  pilU Vibrio cholerae strain A1552

58.286

94.086

0.548
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.678

93.548

0.409
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

89.785

0.374
  pilT Vibrio cholerae strain A1552

41.617

89.785

0.374
  pilT Legionella pneumophila strain ERS1305867

40.525

92.204

0.374
  pilT Legionella pneumophila strain Lp02

40.525

92.204

0.374
  pilT Acinetobacter baylyi ADP1

39.883

91.667

0.366
  pilT Pseudomonas aeruginosa PAK

40.597

90.054

0.366
  pilT Pseudomonas stutzeri DSM 10701

40

90.054

0.36
  pilT Acinetobacter nosocomialis M2

39.528

91.129

0.36
  pilT Acinetobacter baumannii D1279779

39.528

91.129

0.36
  pilT Acinetobacter baumannii strain A118

39.528

91.129

0.36

Multiple sequence alignment