Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BUQ73_RS24710 Genome accession   NZ_CP018846
Coordinates   5463059..5464057 (-) Length   332 a.a.
NCBI ID   WP_079230055.1    Uniprot ID   -
Organism   Pseudomonas putida strain AA7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5458059..5469057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUQ73_RS24690 argS 5459234..5460970 (+) 1737 WP_079230052.1 arginine--tRNA ligase -
  BUQ73_RS24695 - 5460972..5461673 (+) 702 WP_079230053.1 SPOR domain-containing protein -
  BUQ73_RS24700 - 5461730..5462149 (-) 420 WP_079230054.1 NINE protein -
  BUQ73_RS24705 - 5462316..5462945 (+) 630 WP_027920985.1 C40 family peptidase -
  BUQ73_RS24710 pilT 5463059..5464057 (-) 999 WP_079230055.1 type IV pilus twitching motility protein PilT Machinery gene
  BUQ73_RS24715 - 5464124..5464810 (+) 687 WP_079230056.1 YggS family pyridoxal phosphate-dependent enzyme -
  BUQ73_RS24720 proC 5464849..5465667 (+) 819 WP_079230057.1 pyrroline-5-carboxylate reductase -
  BUQ73_RS24725 - 5465678..5466268 (+) 591 WP_079230058.1 YggT family protein -
  BUQ73_RS24730 metX 5466368..5467507 (+) 1140 WP_079230059.1 homoserine O-succinyltransferase MetX -
  BUQ73_RS24735 metW 5467515..5468135 (+) 621 WP_027920979.1 methionine biosynthesis protein MetW -
  BUQ73_RS24740 - 5468158..5468592 (+) 435 WP_027920978.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35715.37 Da        Isoelectric Point: 6.3939

>NTDB_id=210984 BUQ73_RS24710 WP_079230055.1 5463059..5464057(-) (pilT) [Pseudomonas putida strain AA7]
MDVTDLLARAVDAGASDLHLAAGEIPMLRVDGELRRMQLPGVTTAALSDALTPLLDEGQCRQWQQGDELDLALELASLGR
FRLNLFRQLNGLAASFRLIPKRIASVGELDLEEVFQAVARHADGLVLIGGPTGSGKSSTLAALIDRLNGERALHIITLED
PVEVIHSSQCSLVNQREIGRHCRDFGQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAAGSIDRL
IEVFAADEKPLVRAMLAESLRLVVAQVLVKRVGGGRVAAREVLVATPAVRNLVREGRMAQLCSVMQAGAAEGMRTMEGAM
LGLREKGLIGAF

Nucleotide


Download         Length: 999 bp        

>NTDB_id=210984 BUQ73_RS24710 WP_079230055.1 5463059..5464057(-) (pilT) [Pseudomonas putida strain AA7]
ATGGATGTGACCGACCTGTTGGCCCGAGCCGTGGACGCAGGGGCGAGCGACCTGCATCTGGCTGCGGGCGAGATACCGAT
GCTGCGTGTGGATGGCGAGCTGCGGCGCATGCAGCTACCGGGGGTGACGACGGCAGCGTTGAGCGACGCACTGACGCCGC
TGCTCGACGAGGGCCAGTGCCGGCAGTGGCAGCAAGGCGACGAGCTGGATCTGGCATTGGAACTGGCATCGTTGGGGCGT
TTTCGTCTCAATCTGTTTCGGCAACTGAACGGCCTGGCAGCCTCGTTTCGTCTGATTCCGAAACGCATTGCCAGTGTTGG
CGAACTTGATCTTGAAGAAGTGTTTCAAGCCGTTGCGCGGCATGCCGATGGCCTGGTGCTGATAGGCGGCCCGACCGGCA
GCGGCAAGTCGAGCACCCTGGCGGCACTGATTGACCGGCTCAATGGTGAGCGGGCGCTACATATCATCACCCTCGAAGAC
CCTGTTGAAGTTATCCACAGCAGCCAATGCAGCCTGGTCAACCAGCGCGAGATCGGCCGGCATTGTCGCGACTTCGGCCA
AGGGCTGCGCAGCGCTCTGCGTCAAGACCCGGATGTGATCATGATTGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTACGCGCAGCGGAGACGGGGCATCTGGTGCTGGCCACGGTGCATACGCGGTCTGCGGCTGGCAGCATTGATCGGCTG
ATCGAGGTATTTGCGGCGGACGAAAAGCCGCTGGTGCGGGCGATGCTGGCGGAGTCGTTGCGCCTGGTGGTGGCACAGGT
GCTGGTCAAGCGTGTTGGCGGCGGCCGGGTGGCGGCGCGAGAAGTGTTGGTGGCGACACCTGCGGTGCGCAACCTGGTGC
GGGAGGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGGGCGGCGGAGGGGATGCGGACGATGGAGGGGGCGATG
CTTGGGCTGAGGGAGAAAGGATTGATCGGAGCTTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.064

99.096

0.506
  pilT Vibrio cholerae strain A1552

50.755

99.699

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.755

99.699

0.506
  pilT Acinetobacter baylyi ADP1

50.606

99.398

0.503
  pilT Pseudomonas stutzeri DSM 10701

50.456

99.096

0.5
  pilT Acinetobacter baumannii strain A118

50.303

99.398

0.5
  pilT Acinetobacter baumannii D1279779

50.303

99.398

0.5
  pilT Acinetobacter nosocomialis M2

50.303

99.398

0.5
  pilT Legionella pneumophila strain Lp02

50

99.398

0.497
  pilT Legionella pneumophila strain ERS1305867

50

99.398

0.497
  pilT Neisseria meningitidis 8013

49.547

99.699

0.494
  pilT Neisseria gonorrhoeae MS11

49.245

99.699

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.545

99.398

0.443
  pilU Pseudomonas stutzeri DSM 10701

37.576

99.398

0.373

Multiple sequence alignment