Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BSZ04_RS11005 Genome accession   NZ_CP018312
Coordinates   399421..399870 (-) Length   149 a.a.
NCBI ID   WP_088880392.1    Uniprot ID   A0A510I815
Organism   Vibrio rotiferianus strain B64D1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 394421..404870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSZ04_RS10985 (BSZ04_10880) coaE 394940..395554 (-) 615 WP_088880389.1 dephospho-CoA kinase -
  BSZ04_RS10990 (BSZ04_10885) pilD 395555..396424 (-) 870 WP_010450189.1 prepilin peptidase Machinery gene
  BSZ04_RS10995 (BSZ04_10890) pilC 396495..397718 (-) 1224 WP_088880390.1 type II secretion system F family protein Machinery gene
  BSZ04_RS11000 (BSZ04_10895) pilB 397732..399417 (-) 1686 WP_088880391.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BSZ04_RS11005 (BSZ04_10900) pilA 399421..399870 (-) 450 WP_088880392.1 pilin Machinery gene
  BSZ04_RS11010 (BSZ04_10905) nadC 400098..400985 (-) 888 WP_088880393.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BSZ04_RS11015 (BSZ04_10910) ampD 401078..401629 (+) 552 WP_038885735.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BSZ04_RS11020 (BSZ04_10915) pdhR 402034..402801 (+) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15596.10 Da        Isoelectric Point: 10.0439

>NTDB_id=207993 BSZ04_RS11005 WP_088880392.1 399421..399870(-) (pilA) [Vibrio rotiferianus strain B64D1]
MNVTKRKIQKGFTLIELMIVVAVIGVLAAIAIPQYQNYVAKSELGSGLATITSIRTNVEDYVVTNGAFPDGTTAGQKPTD
LGVIQPGNGTIRFVKAKGQLLLTFASTGNSPDVNSAKIALVRASGGTWTCKSTLDAPLLPKSCTKDATL

Nucleotide


Download         Length: 450 bp        

>NTDB_id=207993 BSZ04_RS11005 WP_088880392.1 399421..399870(-) (pilA) [Vibrio rotiferianus strain B64D1]
ATGAACGTAACCAAACGAAAAATACAGAAAGGCTTTACACTGATCGAATTAATGATTGTTGTGGCTGTCATTGGAGTACT
CGCCGCAATCGCAATTCCTCAATATCAAAATTATGTCGCCAAGTCCGAGCTAGGTTCTGGCTTAGCTACCATTACTTCGA
TCAGAACTAACGTTGAAGATTATGTTGTCACCAATGGGGCATTCCCAGATGGCACCACTGCAGGTCAAAAGCCGACAGAC
CTGGGTGTTATTCAACCAGGTAACGGTACAATTCGTTTCGTGAAAGCAAAAGGTCAACTATTGCTTACCTTTGCATCGAC
AGGAAACAGCCCTGACGTAAATAGCGCAAAAATTGCATTGGTTAGAGCTTCAGGCGGTACATGGACTTGTAAATCAACCT
TAGATGCTCCTCTACTGCCTAAGTCCTGCACAAAAGACGCTACGCTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A510I815

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

54.248

100

0.557
  pilA Vibrio cholerae strain A1552

54.248

100

0.557
  pilA Vibrio cholerae C6706

54.248

100

0.557
  pilA Pseudomonas aeruginosa PAK

45.638

100

0.456
  pilA Acinetobacter baumannii strain A118

44.366

95.302

0.423
  pilA Acinetobacter nosocomialis M2

40.127

100

0.423
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.69

97.315

0.396
  pilA Vibrio parahaemolyticus RIMD 2210633

43.704

90.604

0.396
  pilA2 Legionella pneumophila str. Paris

41.135

94.631

0.389
  pilA2 Legionella pneumophila strain ERS1305867

40.426

94.631

0.383
  pilA Haemophilus influenzae 86-028NP

37.255

100

0.383
  comP Acinetobacter baylyi ADP1

39.041

97.987

0.383

Multiple sequence alignment