Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K01M1_RS14025 Genome accession   NZ_CP017889
Coordinates   2943175..2944281 (-) Length   368 a.a.
NCBI ID   WP_086047046.1    Uniprot ID   A0A1W6TUR3
Organism   Vibrio alginolyticus strain K01M1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2938175..2949281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K01M1_RS13995 (K01M1_27030) - 2938808..2939305 (+) 498 WP_005384539.1 SprT family zinc-dependent metalloprotease -
  K01M1_RS14000 (K01M1_27040) endA 2939455..2940150 (+) 696 WP_053306786.1 deoxyribonuclease I -
  K01M1_RS14005 (K01M1_27050) rsmE 2940264..2940995 (+) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K01M1_RS14010 (K01M1_27060) gshB 2941009..2941959 (+) 951 WP_086047045.1 glutathione synthase -
  K01M1_RS14015 (K01M1_27070) - 2942088..2942651 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  K01M1_RS14020 (K01M1_27080) ruvX 2942687..2943112 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  K01M1_RS14025 (K01M1_27090) pilU 2943175..2944281 (-) 1107 WP_086047046.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K01M1_RS14030 (K01M1_27100) pilT 2944312..2945352 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  K01M1_RS14035 (K01M1_27110) - 2945381..2946082 (+) 702 WP_005384521.1 YggS family pyridoxal phosphate-dependent enzyme -
  K01M1_RS14040 (K01M1_27120) proC 2946242..2947060 (+) 819 WP_086047047.1 pyrroline-5-carboxylate reductase -
  K01M1_RS14045 (K01M1_27130) - 2947113..2947670 (+) 558 WP_005381012.1 YggT family protein -
  K01M1_RS14050 (K01M1_27140) yggU 2947670..2947960 (+) 291 WP_086047048.1 DUF167 family protein YggU -
  K01M1_RS14055 (K01M1_27150) - 2948089..2948520 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  K01M1_RS14060 (K01M1_27160) - 2948639..2949241 (+) 603 WP_005381009.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41312.40 Da        Isoelectric Point: 6.4229

>NTDB_id=203669 K01M1_RS14025 WP_086047046.1 2943175..2944281(-) (pilU) [Vibrio alginolyticus strain K01M1]
MDLDKFLEGMLALKASDLYITVGAPILFRVDDELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=203669 K01M1_RS14025 WP_086047046.1 2943175..2944281(-) (pilU) [Vibrio alginolyticus strain K01M1]
ATGGATCTAGATAAATTTCTCGAAGGCATGCTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGAGCGCCAATTCT
ATTTCGTGTGGATGACGAATTGCGTCCGCAAGGGGAAAAACTCACCGAGAGTGATGTGGCTGCATTACTCGATAGCGCTA
TGGAACCAGATCGGCGTCAGGAGTTTCGTAAAAGTCGCGAGTCGAATTTTGCCATTGTGAGAGATTGTGGTCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACTAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCGATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGATCGGGTA
AATCGACCACCATGGCGGCGATGACAGGCTATCGAAACAGCAATAAAACCGGGCACATTTTGACGGTCGAAGATCCGATC
GAATTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGCGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAATAATGCCAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGATTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGTTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTCTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGTGCCGACTCGGCAAATGATCTGCGCTTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6TUR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.065

100

0.821
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.923

88.315

0.397
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Acinetobacter baylyi ADP1

41.39

89.946

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372

Multiple sequence alignment