Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BIZ42_RS11855 Genome accession   NZ_CP017483
Coordinates   2720946..2722073 (-) Length   375 a.a.
NCBI ID   WP_046273783.1    Uniprot ID   A0AAJ6FUW8
Organism   Stenotrophomonas sp. LM091     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2715946..2727073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIZ42_RS11840 (BIZ42_11840) - 2717355..2718743 (-) 1389 WP_070426556.1 DEAD/DEAH box helicase -
  BIZ42_RS11845 (BIZ42_11845) - 2719187..2720176 (+) 990 WP_046273781.1 fumarylacetoacetate hydrolase family protein -
  BIZ42_RS11850 (BIZ42_11850) maiA 2720197..2720850 (+) 654 WP_070426557.1 maleylacetoacetate isomerase -
  BIZ42_RS11855 (BIZ42_11855) pilU 2720946..2722073 (-) 1128 WP_046273783.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BIZ42_RS11860 (BIZ42_11860) - 2722171..2722554 (+) 384 WP_070426558.1 DUF4398 domain-containing protein -
  BIZ42_RS11865 (BIZ42_11865) - 2722617..2723450 (+) 834 WP_237393598.1 hypothetical protein -
  BIZ42_RS11870 (BIZ42_11870) - 2723815..2724033 (+) 219 WP_019182593.1 YdcH family protein -
  BIZ42_RS11875 (BIZ42_11875) - 2724226..2725596 (+) 1371 WP_070426559.1 pyridoxal-phosphate dependent enzyme -
  BIZ42_RS11880 (BIZ42_11880) - 2725716..2726909 (+) 1194 WP_070426560.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41419.69 Da        Isoelectric Point: 6.2377

>NTDB_id=198714 BIZ42_RS11855 WP_046273783.1 2720946..2722073(-) (pilU) [Stenotrophomonas sp. LM091]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQAPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHTFHDALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHRNVLMNLSLNLRAVVSQRLVKGKDGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRMAKNGIIEQEEALRAADSRDGLALKFRLSEGGSEHDPYADYSAASAPAITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=198714 BIZ42_RS11855 WP_046273783.1 2720946..2722073(-) (pilU) [Stenotrophomonas sp. LM091]
ATGGATATCGGCTATTTCCTGAAGCTGATGACCGAAAAGAACGCGTCGGACATGTTCTTGACGACCGGAGCACCGGTATA
CATCAAGATCGAGGGGAAACTCTATCCGCTGGGCAACACCGGGTTGCCGCCCGGGATGGTCAAGAAGATCGCCTACTCGC
TGATGGATGAGGGCCAGGCCCCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATTGCCCTGCCCGACGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCAAGATCCCCAGCATCGA
AGAACTCAACCTGCCCCAGGTCCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGCTCGACCGGCT
CGGGAAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAACACAAGATGTCGATCGTGAACCAGCGCGAGGTCGGGCTGGACACGCACACCTTCCACGA
CGCGTTGAAGAACGCGATGCGTGAAGCGCCCGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAAACCATGGAGGCGG
CCATCGCGTTCGCTGAAACCGGGCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCACACCGCAACGTGCTGATGAACCTCTCCCTGAACCTGCGCGCGGTCGTCTCCCAGCG
CCTGGTCAAGGGCAAGGACGGGCGCCGCCTGCCGGCCACCGAAGTGCTCATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAGGCCTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGATGGCCAAGAACGGCATCATCGAGCAGGAGGAGGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGTTGAA
ATTCCGGCTGTCCGAAGGCGGCAGCGAACACGATCCGTACGCGGATTACTCGGCTGCCTCCGCCCCGGCGATCACGCACG
GGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.733

0.499
  pilU Acinetobacter baylyi ADP1

49.162

95.467

0.469
  pilU Vibrio cholerae strain A1552

48.588

94.4

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.503

100

0.453
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.471

90.667

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

41.124

90.133

0.371
  pilT Legionella pneumophila strain ERS1305867

41.124

90.133

0.371
  pilT Neisseria gonorrhoeae MS11

38.905

92.533

0.36
  pilT Neisseria meningitidis 8013

38.905

92.533

0.36

Multiple sequence alignment