Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FORC55_RS02475 Genome accession   NZ_CP016987
Coordinates   496046..496483 (-) Length   145 a.a.
NCBI ID   WP_096070334.1    Uniprot ID   -
Organism   Vibrio cholerae strain FORC_055 isolate MFDS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 491046..501483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC55_RS02455 (FORC55_0448) coaE 491539..492147 (-) 609 WP_000011549.1 dephospho-CoA kinase -
  FORC55_RS02460 (FORC55_0449) pilD 492144..493019 (-) 876 WP_000418750.1 prepilin peptidase Machinery gene
  FORC55_RS02465 (FORC55_0450) pilC 493077..494303 (-) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  FORC55_RS02470 (FORC55_0451) pilB 494346..496034 (-) 1689 WP_096070333.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC55_RS02475 (FORC55_0452) pilA 496046..496483 (-) 438 WP_096070334.1 pilin Machinery gene
  FORC55_RS02480 (FORC55_0453) nadC 496728..497618 (-) 891 WP_000665275.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC55_RS02490 (FORC55_0454) ampD 497920..498465 (+) 546 WP_084998538.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC55_RS02495 (FORC55_0455) fldB 498596..499114 (-) 519 WP_084988580.1 flavodoxin FldB -
  FORC55_RS02500 (FORC55_0456) xerD 499253..500161 (+) 909 WP_084988579.1 site-specific tyrosine recombinase XerD -
  FORC55_RS02505 (FORC55_0457) dsbC 500178..500930 (+) 753 WP_084998540.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15395.59 Da        Isoelectric Point: 9.3769

>NTDB_id=194311 FORC55_RS02475 WP_096070334.1 496046..496483(-) (pilA) [Vibrio cholerae strain FORC_055 isolate MFDS]
MKAYKNKLQQGFTLIELMIVVAIIGILAAIAVPQYQKYVAKSEAASALASITAHRTNVEMYVVENGTFPATSTALPIPST
TSGNITYTQQASGAGNIIFTFAASGASPSVVNRFVTLERNGEGVWGCKSNIDDAYRPKGCSKQTN

Nucleotide


Download         Length: 438 bp        

>NTDB_id=194311 FORC55_RS02475 WP_096070334.1 496046..496483(-) (pilA) [Vibrio cholerae strain FORC_055 isolate MFDS]
ATGAAAGCGTATAAAAACAAACTACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCGATTATTGGCATTTT
GGCTGCCATTGCCGTACCGCAATATCAGAAATATGTGGCCAAAAGTGAAGCAGCATCAGCACTGGCTTCAATTACCGCAC
ACAGAACGAATGTGGAAATGTACGTGGTAGAAAATGGGACTTTTCCAGCGACATCAACCGCTTTACCAATTCCATCAACA
ACCTCTGGAAATATTACCTATACACAACAGGCTTCGGGGGCAGGTAATATCATCTTTACATTCGCTGCCAGCGGTGCAAG
TCCGAGTGTAGTGAATAGATTTGTCACATTAGAACGTAATGGAGAGGGTGTATGGGGATGTAAGTCAAATATTGATGACG
CTTATAGACCCAAAGGCTGTAGCAAGCAAACAAATTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

47.02

100

0.49
  pilA Vibrio cholerae C6706

47.02

100

0.49
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.02

100

0.49
  pilA Pseudomonas aeruginosa PAK

46.98

100

0.483
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

98.621

0.428
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.478

95.172

0.414
  pilA Acinetobacter baumannii strain A118

41.844

97.241

0.407
  pilA Vibrio parahaemolyticus RIMD 2210633

43.077

89.655

0.386
  pilA2 Legionella pneumophila str. Paris

39.259

93.103

0.366
  pilA2 Legionella pneumophila strain ERS1305867

39.259

93.103

0.366

Multiple sequence alignment