Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC55_RS02470 Genome accession   NZ_CP016987
Coordinates   494346..496034 (-) Length   562 a.a.
NCBI ID   WP_096070333.1    Uniprot ID   -
Organism   Vibrio cholerae strain FORC_055 isolate MFDS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 489346..501034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC55_RS02445 (FORC55_0446) yacG 490412..490609 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  FORC55_RS02450 (FORC55_0447) zapD 490767..491507 (-) 741 WP_000207194.1 cell division protein ZapD -
  FORC55_RS02455 (FORC55_0448) coaE 491539..492147 (-) 609 WP_000011549.1 dephospho-CoA kinase -
  FORC55_RS02460 (FORC55_0449) pilD 492144..493019 (-) 876 WP_000418750.1 prepilin peptidase Machinery gene
  FORC55_RS02465 (FORC55_0450) pilC 493077..494303 (-) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  FORC55_RS02470 (FORC55_0451) pilB 494346..496034 (-) 1689 WP_096070333.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC55_RS02475 (FORC55_0452) pilA 496046..496483 (-) 438 WP_096070334.1 pilin Machinery gene
  FORC55_RS02480 (FORC55_0453) nadC 496728..497618 (-) 891 WP_000665275.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC55_RS02490 (FORC55_0454) ampD 497920..498465 (+) 546 WP_084998538.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC55_RS02495 (FORC55_0455) fldB 498596..499114 (-) 519 WP_084988580.1 flavodoxin FldB -
  FORC55_RS02500 (FORC55_0456) xerD 499253..500161 (+) 909 WP_084988579.1 site-specific tyrosine recombinase XerD -
  FORC55_RS02505 (FORC55_0457) dsbC 500178..500930 (+) 753 WP_084998540.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62015.12 Da        Isoelectric Point: 6.0863

>NTDB_id=194310 FORC55_RS02470 WP_096070333.1 494346..496034(-) (pilB) [Vibrio cholerae strain FORC_055 isolate MFDS]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHAQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVGDEELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=194310 FORC55_RS02470 WP_096070333.1 494346..496034(-) (pilB) [Vibrio cholerae strain FORC_055 isolate MFDS]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAATTAATCAGCGCGACGCAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGAACGTCGGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACGCCCAAGAACTCACCGAGCAACTGA
GCCATATTTTCGGCTTGCCAGAAACCGACCTTAGCCGCTACGACTACGCGCCCCTGTGCCAACAGCTCGGGCTGCGCGAA
CTGATTACCCGCTACGATGCCTTGCCAATTGCCAAACAAGGCAATTTATTGCTGCTCGCGGTCTCAGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGCTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCGG
CGATTCGCCGTTTGTATGGCCGATCTATTCAGGGAGCCGCTAACCAAGGGAAAGAGATCAGCCAAGATGAATTGGCCAAT
CTGGTCAAAGTCGGTGATGAAGAACTTCAATCCATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGTAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATTTTGGTGGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGTTTGCCGCAAGACGGGCGAATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGCGTTTCAACACTCCCAACCTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTACCTCAACGCCCTCAAAAGACCACAAGGGATGATC
TTGATGACAGGCCCCACCGGCAGCGGCAAAACTGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCAAAAATCGGCTTTG
GCTTTGCCGAGGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGTGAAATACGCGATCTGGAAACC
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCACACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCGAATATGGGGGTGGAGCCGTTTAACCTCGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCCTTTCAGCCCAATGAA
ATCTTGTATGAAGCGAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAGCGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.11

100

0.991
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

51.619

93.416

0.482
  pilB Acinetobacter baumannii D1279779

50.772

92.171

0.468
  pilB Acinetobacter baylyi ADP1

50.484

91.993

0.464
  pilF Neisseria gonorrhoeae MS11

45.552

100

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

38.245

100

0.411
  pilF Thermus thermophilus HB27

40.85

96.263

0.393
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37

Multiple sequence alignment