Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FORC55_RS02460 Genome accession   NZ_CP016987
Coordinates   492144..493019 (-) Length   291 a.a.
NCBI ID   WP_000418750.1    Uniprot ID   -
Organism   Vibrio cholerae strain FORC_055 isolate MFDS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 487144..498019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC55_RS02440 (FORC55_0445) parC 488078..490363 (+) 2286 WP_000102515.1 DNA topoisomerase IV subunit A -
  FORC55_RS02445 (FORC55_0446) yacG 490412..490609 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  FORC55_RS02450 (FORC55_0447) zapD 490767..491507 (-) 741 WP_000207194.1 cell division protein ZapD -
  FORC55_RS02455 (FORC55_0448) coaE 491539..492147 (-) 609 WP_000011549.1 dephospho-CoA kinase -
  FORC55_RS02460 (FORC55_0449) pilD 492144..493019 (-) 876 WP_000418750.1 prepilin peptidase Machinery gene
  FORC55_RS02465 (FORC55_0450) pilC 493077..494303 (-) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  FORC55_RS02470 (FORC55_0451) pilB 494346..496034 (-) 1689 WP_096070333.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC55_RS02475 (FORC55_0452) pilA 496046..496483 (-) 438 WP_096070334.1 pilin Machinery gene
  FORC55_RS02480 (FORC55_0453) nadC 496728..497618 (-) 891 WP_000665275.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32461.75 Da        Isoelectric Point: 7.1618

>NTDB_id=194308 FORC55_RS02460 WP_000418750.1 492144..493019(-) (pilD) [Vibrio cholerae strain FORC_055 isolate MFDS]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMVGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=194308 FORC55_RS02460 WP_000418750.1 492144..493019(-) (pilD) [Vibrio cholerae strain FORC_055 isolate MFDS]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGTAGCTTTCTCAA
TGTGGTGATCTACCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATCCCGCTACTGAGTTGGCTAGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGTTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCTACTCACTTTCCGTTCGGGGTGTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTATTGATTGCGGCAACCTTCATCGACTTCGATACCCTGTTGCTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCGGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGT
GGGTTATCTCTCGCTGTGGTCAATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCTCTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(135-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

99.656

100

0.997
  pilD Vibrio campbellii strain DS40M4

75

98.969

0.742
  pilD Acinetobacter nosocomialis M2

53.676

93.471

0.502
  pilD Acinetobacter baumannii D1279779

53.309

93.471

0.498
  pilD Neisseria gonorrhoeae MS11

44.444

98.969

0.44

Multiple sequence alignment