Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BSY15_RS09060 Genome accession   NZ_CP016447
Coordinates   2024862..2025998 (-) Length   378 a.a.
NCBI ID   WP_069104522.1    Uniprot ID   A0A1B3PL95
Organism   Acidovorax sp. RAC01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2019862..2030998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSY15_RS09035 (BSY15_1825) rsmI 2020885..2021835 (-) 951 WP_069104517.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  BSY15_RS09040 (BSY15_1826) - 2021835..2022242 (+) 408 WP_069104518.1 YraN family protein -
  BSY15_RS09045 (BSY15_1827) - 2022387..2022989 (+) 603 WP_069104519.1 SIS domain-containing protein -
  BSY15_RS09050 (BSY15_1828) - 2023010..2023681 (+) 672 WP_069104520.1 BON domain-containing protein -
  BSY15_RS09055 (BSY15_1829) - 2023758..2024672 (-) 915 WP_069104521.1 NAD(P)-dependent oxidoreductase -
  BSY15_RS09060 (BSY15_1830) pilU 2024862..2025998 (-) 1137 WP_069104522.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BSY15_RS09065 (BSY15_1831) - 2026066..2026701 (-) 636 WP_069104523.1 cyclic nucleotide-binding domain-containing protein -
  BSY15_RS09070 (BSY15_1832) pilT 2026760..2027803 (-) 1044 WP_069104524.1 type IV pilus twitching motility protein PilT Machinery gene
  BSY15_RS09075 (BSY15_1833) - 2027842..2028567 (+) 726 WP_069104525.1 YggS family pyridoxal phosphate-dependent enzyme -
  BSY15_RS09080 (BSY15_1834) ltaE 2028627..2029691 (-) 1065 WP_069104526.1 low-specificity L-threonine aldolase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41945.29 Da        Isoelectric Point: 6.7683

>NTDB_id=188321 BSY15_RS09060 WP_069104522.1 2024862..2025998(-) (pilU) [Acidovorax sp. RAC01]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLTPNHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGHVGMVMRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSYG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLISDLIFKGDVTEIKEIMKKSRNLG
MQTFDQALFDAYEANVISYEDALRNADSLNDLRLQIKLNSQRARSPDLAQGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=188321 BSY15_RS09060 WP_069104522.1 2024862..2025998(-) (pilU) [Acidovorax sp. RAC01]
ATGGAACGCGATCAGGCCAGTAAATTCATCAATGACCTGCTCAAGCTGATGGTGAGCCGCAATGGCAGCGACCTCTTCAT
CACCGCCGAGTTTCCACCCGCCATCAAGGTGGATGGCAAAGTCACCAAGGTATCGCCCCAGCCGCTGACGCCCAACCACA
CGCTCACGCTGGCCCGCGCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAACGCACCAAGGAGTGCAACTTCGCGATC
TCGCCCGCCGGTATCGGGCGCTTTCGCGTCAATGCCTTCATCCAGCAAGGCCATGTAGGCATGGTCATGCGGACCATTCC
GCTGACGCTTCCCACCATCGACGGACTGGGCGTGCCGCAGGTGCTCAAGGAAGTGACCATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGTGCCACGGGCTCGGGCAAGTCAACCACGCTGGCCGCCATGGTGGATTGGCGCAACGAGAACTCCTACGGC
CACATCATCACGGTGGAAGACCCGATCGAATTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTGGGCCT
GGATACCGACAGCTGGGAAGCGGCCCTGAAAAACACGTTGCGCCAGGCACCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCGGTGGCGTTTGCCGAAACCGGCCACCTGTGCCTGGCTACGCTGCACGCCAACAGTGCC
AACCAGGCGCTGGACCGTATCATCAACTTCTTCCCGGAAGAGCGCCGCGCACAGCTGCTGATGGACCTGTCGCTCAACCT
GCGCGCCATGGTCTCGCAGCGGCTGATTCCCAAGCAGGACGGCAAGGGCCGCGCCGCAGCCGTGGAGGTGATGCTGAACA
CGCCGCTGATCTCCGACCTGATCTTCAAGGGTGACGTCACCGAGATCAAGGAGATCATGAAAAAGAGCCGCAACCTGGGC
ATGCAGACGTTTGACCAGGCGCTGTTCGATGCCTACGAGGCCAACGTCATCAGCTACGAAGACGCCCTGCGCAACGCCGA
TTCGCTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCCCGATCACCCGACCTGGCCCAGGGCACCGAGC
ACTTCGCGATCGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B3PL95

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

58.38

94.709

0.553
  pilU Vibrio cholerae strain A1552

52.342

96.032

0.503
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Legionella pneumophila strain Lp02

44.444

88.095

0.392
  pilT Legionella pneumophila strain ERS1305867

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

44.444

88.095

0.392
  pilT Acinetobacter baumannii strain A118

44.444

88.095

0.392
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

43.887

84.392

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.533

83.862

0.365
  pilT Vibrio cholerae strain A1552

43.533

83.862

0.365

Multiple sequence alignment