Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AL475_RS17745 Genome accession   NZ_CP014033
Coordinates   2040884..2041306 (-) Length   140 a.a.
NCBI ID   WP_020332201.1    Uniprot ID   S7HVZ5
Organism   Vibrio fluvialis strain FDAARGOS_100     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2035884..2046306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL475_RS17725 (AL475_09765) coaE 2036387..2036995 (-) 609 WP_104966494.1 dephospho-CoA kinase -
  AL475_RS17730 (AL475_09770) pilD 2036998..2037867 (-) 870 WP_044366750.1 A24 family peptidase Machinery gene
  AL475_RS17735 (AL475_09775) pilC 2037919..2039145 (-) 1227 WP_104966495.1 type II secretion system F family protein Machinery gene
  AL475_RS17740 (AL475_09780) pilB 2039181..2040873 (-) 1693 Protein_1826 type IV-A pilus assembly ATPase PilB -
  AL475_RS17745 (AL475_09785) pilA 2040884..2041306 (-) 423 WP_020332201.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AL475_RS17750 (AL475_09790) nadC 2041539..2042426 (-) 888 WP_104966496.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL475_RS17755 (AL475_09795) ampD 2042579..2043130 (+) 552 WP_032081227.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AL475_RS17760 (AL475_09800) fldB 2043135..2043656 (-) 522 WP_020430452.1 flavodoxin FldB -
  AL475_RS17765 (AL475_09805) xerD 2043851..2044705 (+) 855 WP_230857653.1 site-specific tyrosine recombinase XerD -
  AL475_RS17770 (AL475_09810) dsbC 2044721..2045485 (+) 765 WP_020430455.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14655.60 Da        Isoelectric Point: 8.4715

>NTDB_id=167665 AL475_RS17745 WP_020332201.1 2040884..2041306(-) (pilA) [Vibrio fluvialis strain FDAARGOS_100]
MNANQRKQQQGFTLIELMIVVAIIGVLSAIAVPAYKNYVSKSELASGFATLKSLVTPAELYIQENGSLSGATSSTFGVVS
GGTNSIGSVNVDPTNKKLTFSFSNSSIDGTTIDYNRSSGAWICTVTFASGITLDKPKSCS

Nucleotide


Download         Length: 423 bp        

>NTDB_id=167665 AL475_RS17745 WP_020332201.1 2040884..2041306(-) (pilA) [Vibrio fluvialis strain FDAARGOS_100]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCCATCATCGGAGTTCT
GTCAGCGATTGCCGTACCCGCTTATAAAAACTACGTATCCAAAAGTGAACTCGCTTCTGGCTTCGCAACTTTAAAATCAT
TAGTTACACCAGCAGAACTTTACATACAAGAAAACGGTTCTTTAAGTGGTGCTACATCTTCAACTTTCGGAGTTGTAAGC
GGTGGGACAAACTCAATTGGCTCTGTTAACGTTGATCCAACGAATAAGAAACTAACTTTTAGTTTCAGTAATAGCTCTAT
AGATGGAACGACTATCGACTACAATCGTTCATCTGGCGCATGGATATGTACCGTAACATTTGCATCAGGGATTACTTTGG
ATAAACCCAAAAGCTGCTCGTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7HVZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

49.275

98.571

0.486
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.355

100

0.436
  pilA Vibrio cholerae strain A1552

39.355

100

0.436
  pilA Vibrio cholerae C6706

39.355

100

0.436
  pilA Pseudomonas aeruginosa PAK

35.762

100

0.386
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.986

100

0.386
  pilA Acinetobacter baumannii strain A118

41.538

92.857

0.386
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.41

99.286

0.371
  pilA Vibrio campbellii strain DS40M4

36.691

99.286

0.364
  pilA/pilA1 Eikenella corrodens VA1

34

100

0.364
  comP Acinetobacter baylyi ADP1

38.931

93.571

0.364

Multiple sequence alignment