Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BC375_RS10885 Genome accession   NZ_CP016610
Coordinates   2248617..2249054 (+) Length   145 a.a.
NCBI ID   WP_046418469.1    Uniprot ID   -
Organism   Xylella fastidiosa strain Salento-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2243617..2254054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BC375_RS10860 (BC375_10175) coaE 2243719..2244342 (-) 624 WP_046418843.1 dephospho-CoA kinase -
  BC375_RS10865 (BC375_10180) pilD 2244354..2245217 (-) 864 WP_010894958.1 A24 family peptidase Machinery gene
  BC375_RS10870 (BC375_10185) pilC 2245224..2246426 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  BC375_RS10875 (BC375_10190) pilA/pilAI 2246875..2247321 (+) 447 WP_010894960.1 pilin Machinery gene
  BC375_RS10880 (BC375_10195) - 2247582..2247785 (-) 204 WP_230950314.1 hypothetical protein -
  BC375_RS10885 (BC375_10200) pilA 2248617..2249054 (+) 438 WP_046418469.1 pilin Machinery gene
  BC375_RS10895 (BC375_10210) pilB 2249892..2251625 (+) 1734 WP_046418464.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BC375_RS10900 (BC375_10215) - 2251719..2253131 (-) 1413 WP_046418462.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15064.52 Da        Isoelectric Point: 8.5119

>NTDB_id=160761 BC375_RS10885 WP_046418469.1 2248617..2249054(+) (pilA) [Xylella fastidiosa strain Salento-2]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVARSQVTAGLAEISPGRAQAEIRIAEARATVLPADIGLRTPTPRCAI
TVNIAANADSIITCTLIGNGQVNNRFVRLTRGVDTTAGAGGQWTCTTDVAEALRPVGCTGVATGG

Nucleotide


Download         Length: 438 bp        

>NTDB_id=160761 BC375_RS10885 WP_046418469.1 2248617..2249054(+) (pilA) [Xylella fastidiosa strain Salento-2]
ATGAAAAAGCAACAAGGCTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATCGCCATCCTGGCAGCGATCGCTCT
GCCCATGTACCAAAATTATGTCGCCAGGTCTCAAGTCACAGCGGGACTGGCCGAAATCTCACCAGGCAGAGCACAAGCCG
AAATCCGTATTGCCGAGGCACGGGCAACAGTTCTTCCGGCCGATATCGGTCTACGCACCCCAACCCCTCGTTGCGCCATT
ACTGTCAACATCGCCGCCAATGCTGACAGCATAATTACCTGTACGCTTATTGGTAATGGCCAAGTCAACAATCGATTCGT
CAGGTTGACTCGCGGTGTCGACACCACCGCAGGCGCAGGAGGTCAGTGGACCTGTACCACAGACGTTGCCGAAGCATTGA
GACCAGTAGGCTGCACTGGTGTTGCTACTGGTGGTTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.755

98.621

0.441
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.262

97.241

0.421
  pilA Pseudomonas aeruginosa PAK

37.58

100

0.407
  pilA Vibrio cholerae strain A1552

36.42

100

0.407
  pilA Vibrio cholerae C6706

36.42

100

0.407
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.42

100

0.407
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.845

97.931

0.4
  pilA Haemophilus influenzae Rd KW20

36.364

100

0.386
  pilA2 Legionella pneumophila str. Paris

40

96.552

0.386
  pilA2 Legionella pneumophila strain ERS1305867

39.286

96.552

0.379
  pilA Vibrio parahaemolyticus RIMD 2210633

38.732

97.931

0.379
  comP Acinetobacter baylyi ADP1

34.615

100

0.372